##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AcsI AflII AgeI
ApoI AscI AseI AsnI AvrII
BbrPI BbsI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiWI BspDI BspLU11I BsrBI
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DrdI EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SmlI SnaBI SpeI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3768 GGTACC G^GTACC
AccIII 3240 TCCGGA T^CCGGA
AclI 509 AACGTT AA^CGTT
AflIII 2469 ACGTGT A^C[GA][CT]GT
AhdI 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 955 GTGCAC G^TGCAC
ApaI 2642 GGGCCC GGGCC^C
ApaLI 955 GTGCAC G^TGCAC
Asp700I 3498 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3768 GGTACC G^GTACC
AspEI 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2816 GCAACGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 949 AGATCT A^GATCT
BsaWI 3240 TCCGGA [AT]^CCGG[AT]
BseAI 3240 TCCGGA T^CCGGA
BsmI 2940 GAATGC GAATGC(1/-1)
Bsp1407I 3809 TGTACA T^GTACA
BspEI 3240 TCCGGA T^CCGGA
BsrGI 3809 TGTACA T^GTACA
BssHII 2633 GCGCGC G^CGCGC
DraI 2086 TTTAAA TTT^AAA
DraIII 3544 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1397 GTCAAC GT[CT]^[GA]AC
HindII 1397 GTCAAC GT[CT]^[GA]AC
KpnI 3768 GGTACC GGTAC^C
KspI 2548 CCGCGG CCGC^GG
MroI 3240 TCCGGA T^CCGGA
MseI 2087 TTAA T^TAA
NcoI 3699 CCATGG C^CATGG
PshAI 925 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 509 AACGTT AA^CGTT
SacII 2548 CCGCGG CCGC^GG
SapI 3624 GCTCTTC GCTCTTC(1/4)
SexAI 1743 ACCAGGT A^CC[AT]GGT
SspBI 3809 TGTACA T^GTACA
SspI 860 AATATT AAT^ATT
TfiI 3345 GATTC G^A[AT]TC
Tru9I 2087 TTAA T^TAA
XmnI 3498 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 711 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2591 CAGATCCTG
Aor51HI 2970 AGCGCT AGC^GCT
3359 AGCGCT
AspI 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3370 GACATCGTC
AviII 2685 TGCGCA TGC^GCA
3152 TGCGCA
BalI 102 TGGCCA TGG^CCA
1605 TGGCCA
BamHI 498 GGATCC G^GATCC
2409 GGATCC
BbvCI 189 GCTGAGG CCTCAGC(-5/-2)
1781 GCTGAGG
BsmBI 300 CGTCTC CGTCTC(1/5)
2674 CGTCTC
BspHI 3227 TCATGA T^CATGA
3348 TCATGA
BsrDI 250 CATTGC GCAATG(2/0)
3820 GCAATG
DsaI 2548 CCGCGG C^C[GA][CT]GG
3699 CCATGG
EagI 2523 CGGCCG C^GGCCG
2562 CGGCCG
EclXI 2523 CGGCCG C^GGCCG
2562 CGGCCG
Eco47III 2970 AGCGCT AGC^GCT
3359 AGCGCT
Eco52I 2523 CGGCCG C^GGCCG
2562 CGGCCG
FspI 2685 TGCGCA TGC^GCA
3152 TGCGCA
MluNI 102 TGGCCA TGG^CCA
1605 TGGCCA
MscI 102 TGGCCA TGG^CCA
1605 TGGCCA
NdeI 2001 CATATG CA^TATG
3394 CATATG
PflFI 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3370 GACATCGTC
PvuII 2483 CAGCTG CAG^CTG
3016 CAGCTG
RcaI 3227 TCATGA T^CATGA
3348 TCATGA
SgrAI 1637 CACCGGCG C[GA]^CCGG[CT]G
2496 CGCCGGTG
SphI 1980 GCATGC GCATG^C
3592 GCATGC
Tth111I 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3370 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AocI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI