################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII AccI AcsI AflII AgeI ApoI AscI AseI AsnI AvrII BbrPI BbsI BciVI BclI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiWI BspDI BspLU11I BsrBI BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DrdI EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SalI SbfI ScaI SfiI SfuI SmlI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3768 GGTACC G^GTACC AccIII 3240 TCCGGA T^CCGGA AclI 509 AACGTT AA^CGTT AflIII 2469 ACGTGT A^C[GA][CT]GT AhdI 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 955 GTGCAC G^TGCAC ApaI 2642 GGGCCC GGGCC^C ApaLI 955 GTGCAC G^TGCAC Asp700I 3498 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3768 GGTACC G^GTACC AspEI 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 2816 GCAACGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 949 AGATCT A^GATCT BsaWI 3240 TCCGGA [AT]^CCGG[AT] BseAI 3240 TCCGGA T^CCGGA BsmI 2940 GAATGC GAATGC(1/-1) Bsp1407I 3809 TGTACA T^GTACA BspEI 3240 TCCGGA T^CCGGA BsrGI 3809 TGTACA T^GTACA BssHII 2633 GCGCGC G^CGCGC DraI 2086 TTTAAA TTT^AAA DraIII 3544 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2393 GACCTGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 1397 GTCAAC GT[CT]^[GA]AC HindII 1397 GTCAAC GT[CT]^[GA]AC KpnI 3768 GGTACC GGTAC^C KspI 2548 CCGCGG CCGC^GG MroI 3240 TCCGGA T^CCGGA MseI 2087 TTAA T^TAA NcoI 3699 CCATGG C^CATGG PshAI 925 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 509 AACGTT AA^CGTT SacII 2548 CCGCGG CCGC^GG SapI 3624 GCTCTTC GCTCTTC(1/4) SexAI 1743 ACCAGGT A^CC[AT]GGT SspBI 3809 TGTACA T^GTACA SspI 860 AATATT AAT^ATT TfiI 3345 GATTC G^A[AT]TC Tru9I 2087 TTAA T^TAA XmnI 3498 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 711 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 2591 CAGATCCTG Aor51HI 2970 AGCGCT AGC^GCT 3359 AGCGCT AspI 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3370 GACATCGTC AviII 2685 TGCGCA TGC^GCA 3152 TGCGCA BalI 102 TGGCCA TGG^CCA 1605 TGGCCA BamHI 498 GGATCC G^GATCC 2409 GGATCC BbvCI 189 GCTGAGG CCTCAGC(-5/-2) 1781 GCTGAGG BsmBI 300 CGTCTC CGTCTC(1/5) 2674 CGTCTC BspHI 3227 TCATGA T^CATGA 3348 TCATGA BsrDI 250 CATTGC GCAATG(2/0) 3820 GCAATG DsaI 2548 CCGCGG C^C[GA][CT]GG 3699 CCATGG EagI 2523 CGGCCG C^GGCCG 2562 CGGCCG EclXI 2523 CGGCCG C^GGCCG 2562 CGGCCG Eco47III 2970 AGCGCT AGC^GCT 3359 AGCGCT Eco52I 2523 CGGCCG C^GGCCG 2562 CGGCCG FspI 2685 TGCGCA TGC^GCA 3152 TGCGCA MluNI 102 TGGCCA TGG^CCA 1605 TGGCCA MscI 102 TGGCCA TGG^CCA 1605 TGGCCA NdeI 2001 CATATG CA^TATG 3394 CATATG PflFI 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3370 GACATCGTC PvuII 2483 CAGCTG CAG^CTG 3016 CAGCTG RcaI 3227 TCATGA T^CATGA 3348 TCATGA SgrAI 1637 CACCGGCG C[GA]^CCGG[CT]G 2496 CGCCGGTG SphI 1980 GCATGC GCATG^C 3592 GCATGC Tth111I 451 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3370 GACATCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AocI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI