##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbrPI BcgI
BfrI BlnI BsaAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI DraIII Eam1105I Eco47III
EcoRI EcoT22I FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1098 GACGTC GACGT^C
AccIII 1102 TCCGGA T^CCGGA
Asp700I 2093 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 229 GGATCC G^GATCC
BbeI 3104 GGCGCC GGCGC^C
BciVI 1664 GTATCC GTATCC(6/5)
BclI 3947 TGATCA T^GATCA
BglII 502 AGATCT A^GATCT
BlpI 627 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 627 GCTTAGC GC^T[ACGT]AGC
BseAI 1102 TCCGGA T^CCGGA
BspEI 1102 TCCGGA T^CCGGA
BsrBI 2186 CCGCTC CCGCTC(-3/-3)
BsrDI 2831 CATTGC GCAATG(2/0)
BssSI 3693 CACGAG CACGAG(-5/-1)
BstBI 849 TTCGAA TT^CGAA
BstEII 36 GGTGACC G^GT[ACGT]ACC
BstPI 36 GGTGACC G^GT[ACGT]ACC
CelII 627 GCTTAGC GC^T[ACGT]AGC
DrdI 4015 GACCGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
EcoRV 3564 GATATC GAT^ATC
FbaI 3947 TGATCA T^GATCA
KasI 3104 GGCGCC G^GCGCC
KspI 1702 CCGCGG CCGC^GG
MroI 1102 TCCGGA T^CCGGA
NaeI 74 GCCGGC GCC^GGC
NarI 3104 GGCGCC GG^CGCC
NgoMIV 74 GCCGGC G^CCGGC
NspV 849 TTCGAA TT^CGAA
SacI 21 GAGCTC GAGCT^C
SacII 1702 CCGCGG CCGC^GG
SapI 611 GAAGAGC GCTCTTC(1/4)
SfiI 1211 GGCCCAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3104 GGCGCC GGC^GCC
SfuI 849 TTCGAA TT^CGAA
SmlI 2918 CTTGAG C^T[CT][GA]AG
SphI 1318 GCATGC GCATG^C
XmnI 2093 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 896 GTAC GT^AC
3399 GTAC
Alw44I 1139 GTGCAC G^TGCAC
2714 GTGCAC
ApaLI 1139 GTGCAC G^TGCAC
2714 GTGCAC
BbsI 983 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BpuAI 983 GAAGAC GAAGAC(2/6)
1047 GAAGAC
BsaWI 1051 ACCGGA [AT]^CCGG[AT]
1102 TCCGGA
BsiEI 8 CGGCCG CG[GA][CT]^CG
3041 CGGCCG
BstAPI 1171 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3181 GCAAACGCTGC
DraI 4077 TTTAAA TTT^AAA
4117 TTTAAA
EagI 8 CGGCCG C^GGCCG
3041 CGGCCG
EclXI 8 CGGCCG C^GGCCG
3041 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
3041 CGGCCG
HincII 1605 GTCAAC GT[CT]^[GA]AC
2007 GTTGAC
HindII 1605 GTCAAC GT[CT]^[GA]AC
2007 GTTGAC
MaeII 1099 ACGT A^CGT
1984 ACGT
MslI 2497 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3253 CATGGCTATG
NspI 1318 GCATGC [GA]CATG^[CT]
1345 GCATGT
PflMI 460 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2446 CCACTCCCTGG
PstI 1722 CTGCAG CTGCA^G
2222 CTGCAG
PvuII 667 CAGCTG CAG^CTG
2340 CAGCTG
RsaI 896 GTAC GT^AC
3399 GTAC
Van91I 460 CCAGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2446 CCACTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI