##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI AspI AvrII BbrPI BbvCI
BcgI BclI BglII BlnI BsaAI
BsaBI BseAI BsiWI BspDI BspEI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI Eco47III EcoO65I
EcoRI EcoRV FbaI FseI KspI
MamI MfeI MluI MroI MunI
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SfiI SfuI
SnaBI SpeI SphI SwaI Tth111I
Van91I
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 884 GGTACC G^GTACC
AccI 105 GTCTAC GT^[AC][GT]AC
AflII 1874 CTTAAG C^TTAAG
AhdI 3580 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 884 GGTACC G^GTACC
AspEI 3580 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1617 TGCGCA TGC^GCA
BalI 3248 TGGCCA TGG^CCA
BamHI 2962 GGATCC G^GATCC
BfrI 1874 CTTAAG C^TTAAG
BglI 1370 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 380 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 380 GCTAAGC GC^T[ACGT]AGC
BsgI 2738 GTGCAG GTGCAG(16/14)
BsiEI 1319 CGGCCG CG[GA][CT]^CG
BsmBI 1109 GAGACG CGTCTC(1/5)
BssHII 1229 GCGCGC G^CGCGC
BssSI 3868 CTCGTG CACGAG(-5/-1)
BstAPI 576 GCAGGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 380 GCTAAGC GC^T[ACGT]AGC
DraIII 2451 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1319 CGGCCG C^GGCCG
Eam1105I 3580 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1319 CGGCCG C^GGCCG
Eco52I 1319 CGGCCG C^GGCCG
EcoNI 1478 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1617 TGCGCA TGC^GCA
HindIII 2335 AAGCTT A^AGCTT
HpaI 3422 GTTAAC GTT^AAC
KpnI 884 GGTACC GGTAC^C
MluNI 3248 TGGCCA TGG^CCA
MscI 3248 TGGCCA TGG^CCA
MspA1I 1665 CAGCTG C[AC]G^C[GT]G
NaeI 1391 GCCGGC GCC^GGC
NcoI 1381 CCATGG C^CATGG
NgoMIV 1391 GCCGGC G^CCGGC
PaeR7I 2299 CTCGAG C^TCGAG
PshAI 1280 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1665 CAGCTG CAG^CTG
SbfI 1504 CCTGCAGG CCTGCA^GG
SexAI 3414 ACCTGGT A^CC[AT]GGT
SgrAI 1390 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1311 CCCGGG CCC^GGG
Sse8387I 1504 CCTGCAGG CCTGCA^GG
XbaI 3525 TCTAGA T^CTAGA
XhoI 2299 CTCGAG C^TCGAG
XmaCI 1311 CCCGGG C^CCGGG
XmaI 1311 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
Alw44I 3826 GTGCAC G^TGCAC
3898 GTGCAC
AlwNI 252 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1575 CAGGAACTG
AocI 30 CCTTAGG CC^T[ACGT]AGG
656 CCTTAGG
ApaI 1377 GGGCCC GGGCC^C
2017 GGGCCC
ApaLI 3826 GTGCAC G^TGCAC
3898 GTGCAC
AseI 325 ATTAAT AT^TAAT
3466 ATTAAT
AsnI 325 ATTAAT AT^TAAT
3466 ATTAAT
Asp700I 2165 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3005 GAAAGCCTTC
AvaI 1311 CCCGGG C^[CT]CG[GA]G
2299 CTCGAG
BbeI 1241 GGCGCC GGCGC^C
1388 GGCGCC
BbsI 1416 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BciVI 705 GGATAC GTATCC(6/5)
87 GTATCC
BpuAI 1416 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BsaWI 2214 ACCGGA [AT]^CCGG[AT]
2634 ACCGGA
BseRI 2351 CTCCTC GAGGAG(10/8)
1121 GAGGAG
BsoBI 1311 CCCGGG C^[CT]CG[GA]G
2299 CTCGAG
Bsp1407I 3833 TGTACA T^GTACA
3841 TGTACA
BspHI 204 TCATGA T^CATGA
3806 TCATGA
BsrBI 2264 CCGCTC CCGCTC(-3/-3)
2432 CCGCTC
BsrFI 1335 ACCGGC [GA]^CCGG[CT]
1391 GCCGGC
BsrGI 3833 TGTACA T^GTACA
3841 TGTACA
BstXI 406 CCAAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1367 CCAGCCCCCTGG
BstYI 1713 AGATCC [GA]^GATC[CT]
2962 GGATCC
Bsu36I 30 CCTTAGG CC^T[ACGT]AGG
656 CCTTAGG
Cfr10I 1335 ACCGGC [GA]^CCGG[CT]
1391 GCCGGC
DsaI 783 CCACGG C^C[GA][CT]GG
1381 CCATGG
EaeI 1319 CGGCCG [CT]^GGCC[GA]
3248 TGGCCA
Eco81I 30 CCTTAGG CC^T[ACGT]AGG
656 CCTTAGG
EcoT22I 153 ATGCAT ATGCA^T
3428 ATGCAT
HaeII 1241 GGCGCC [GA]GCGC^[CT]
1388 GGCGCC
HgaI 1011 GCGTC GACGC(5/10)
1178 GCGTC
KasI 1241 GGCGCC G^GCGCC
1388 GGCGCC
MflI 1713 AGATCC [GA]^GATC[CT]
2962 GGATCC
NarI 1241 GGCGCC GG^CGCC
1388 GGCGCC
NsiI 153 ATGCAT ATGCA^T
3428 ATGCAT
PshBI 325 ATTAAT AT^TAAT
3466 ATTAAT
PstI 38 CTGCAG CTGCA^G
1505 CTGCAG
RcaI 204 TCATGA T^CATGA
3806 TCATGA
SfoI 1241 GGCGCC GGC^GCC
1388 GGCGCC
SspBI 3833 TGTACA T^GTACA
3841 TGTACA
XcmI 403 CCACCAAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1055 CCAACGCTACACTGG
XhoII 1713 AGATCC [GA]^GATC[CT]
2962 GGATCC
XmnI 2165 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3005 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I