##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AocI AscI AvrII BbrPI
BclI BfrI BglII BlnI BsaAI
BsaWI BseAI BsiEI BspDI BspEI
BspLU11I BsrDI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI EagI EclXI Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3881 GGTACC G^GTACC
AccI 3809 GTAGAC GT^[AC][GT]AC
AccII 2391 CGCG CG^CG
Alw44I 985 GTGCAC G^TGCAC
Aor51HI 1076 AGCGCT AGC^GCT
ApaLI 985 GTGCAC G^TGCAC
AseI 3155 ATTAAT AT^TAAT
AsnI 3155 ATTAAT AT^TAAT
Asp700I 4469 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3881 GGTACC G^GTACC
AspI 3413 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2357 TGCGCA TGC^GCA
BbvCI 3469 CCTCAGC CCTCAGC(-5/-2)
BsaBI 4249 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 666 CGTACG C^GTACG
BsmI 124 GAATGC GAATGC(1/-1)
Bsp1407I 4395 TGTACA T^GTACA
BspHI 1844 TCATGA T^CATGA
BsrGI 4395 TGTACA T^GTACA
BstBI 3680 TTCGAA TT^CGAA
BstUI 2391 CGCG CG^CG
Eco47III 1076 AGCGCT AGC^GCT
EcoRI 3121 GAATTC G^AATTC
FspI 2357 TGCGCA TGC^GCA
KpnI 3881 GGTACC GGTAC^C
MamI 4249 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2391 CGCG CG^CG
NspI 2336 GCATGC [GA]CATG^[CT]
NspV 3680 TTCGAA TT^CGAA
PflFI 3413 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3155 ATTAAT AT^TAAT
RcaI 1844 TCATGA T^CATGA
SexAI 1386 ACCAGGT A^CC[AT]GGT
SfuI 3680 TTCGAA TT^CGAA
SphI 2336 GCATGC GCATG^C
SspBI 4395 TGTACA T^GTACA
Tth111I 3413 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4469 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AhdI 1835 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4225 GACTTCAGGTC
AspEI 1835 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4225 GACTTCAGGTC
BamHI 913 GGATCC G^GATCC
4254 GGATCC
BbeI 860 GGCGCC GGCGC^C
1432 GGCGCC
BbsI 1364 GTCTTC GAAGAC(2/6)
2719 GTCTTC
BcgI 811 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
225 GCAGCAACCTCG
BciVI 3627 GGATAC GTATCC(6/5)
3874 GTATCC
BpuAI 1364 GTCTTC GAAGAC(2/6)
2719 GTCTTC
BsrFI 2041 GCCGGT [GA]^CCGG[CT]
2265 GCCGGT
BssSI 754 CACGAG CACGAG(-5/-1)
2554 CACGAG
Cfr10I 2041 GCCGGT [GA]^CCGG[CT]
2265 GCCGGT
DrdI 1638 GACTTTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1731 GACACTTACGTC
Eam1105I 1835 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4225 GACTTCAGGTC
HincII 137 GTCAAC GT[CT]^[GA]AC
4124 GTTGAC
HindII 137 GTCAAC GT[CT]^[GA]AC
4124 GTTGAC
KasI 860 GGCGCC G^GCGCC
1432 GGCGCC
NarI 860 GGCGCC GG^CGCC
1432 GGCGCC
PaeR7I 1349 CTCGAG C^TCGAG
1569 CTCGAG
SfoI 860 GGCGCC GGC^GCC
1432 GGCGCC
TfiI 2196 GATTC G^A[AT]TC
3225 GAATC
XhoI 1349 CTCGAG C^TCGAG
1569 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI