##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BciVI BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI Eco81I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HgaI KpnI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SexAI SfuI SgrAI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2227 GTAGAC GT^[AC][GT]AC
AcyI 84 GGCGCC G[GA]^CG[CT]C
AflII 2086 CTTAAG C^TTAAG
AhdI 3469 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2001 AGCGCT AGC^GCT
Asp700I 4358 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3469 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 84 GGCGCC GGCGC^C
BbvCI 722 CCTCAGC CCTCAGC(-5/-2)
BclI 2310 TGATCA T^GATCA
BfrI 2086 CTTAAG C^TTAAG
BglI 353 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2317 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2317 GCTCAGC GC^T[ACGT]AGC
BsaHI 84 GGCGCC G[GA]^CG[CT]C
BsiEI 2629 CGGCCG CG[GA][CT]^CG
BsmI 2389 GAATGC GAATGC(1/-1)
BspHI 462 TCATGA T^CATGA
BsrFI 3062 ACCGGC [GA]^CCGG[CT]
BssSI 247 CACGAG CACGAG(-5/-1)
BstAPI 1285 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2317 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3062 ACCGGC [GA]^CCGG[CT]
DraIII 1277 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2629 CGGCCG C^GGCCG
Eam1105I 3469 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2629 CGGCCG C^GGCCG
Eco47III 2001 AGCGCT AGC^GCT
Eco52I 2629 CGGCCG C^GGCCG
FbaI 2310 TGATCA T^GATCA
Hin1I 84 GGCGCC G[GA]^CG[CT]C
HpaI 3123 GTTAAC GTT^AAC
KasI 84 GGCGCC G^GCGCC
KspI 423 CCGCGG CCGC^GG
MaeII 4360 ACGT A^CGT
NarI 84 GGCGCC GG^CGCC
NdeI 1028 CATATG CA^TATG
RcaI 462 TCATGA T^CATGA
SacII 423 CCGCGG CCGC^GG
SapI 110 GCTCTTC GCTCTTC(1/4)
ScaI 3522 AGTACT AGT^ACT
SfiI 352 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 84 GGCGCC GGC^GCC
SmaI 91 CCCGGG CCC^GGG
XmaCI 91 CCCGGG C^CCGGG
XmaI 91 CCCGGG C^CCGGG
XmnI 4358 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 39 CGCG CG^CG
424 CGCG
AlwNI 1933 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2358 CAGTATCTG
AvaI 91 CCCGGG C^[CT]CG[GA]G
1511 CTCGGG
BalI 2469 TGGCCA TGG^CCA
2516 TGGCCA
BbsI 3115 GAAGAC GAAGAC(2/6)
298 GTCTTC
BglII 916 AGATCT A^GATCT
4147 AGATCT
BpuAI 3115 GAAGAC GAAGAC(2/6)
298 GTCTTC
BsoBI 91 CCCGGG C^[CT]CG[GA]G
1511 CTCGGG
BspMI 2789 ACCTGC ACCTGC(4/8)
2717 GCAGGT
BsrBI 250 GAGCGG CCGCTC(-3/-3)
1442 GAGCGG
BstUI 39 CGCG CG^CG
424 CGCG
BstXI 192 CCACTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1307 CCAGCCGACTGG
DrdI 148 GACTCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3672 GACTGGATGGTC
EcoT22I 2391 ATGCAT ATGCA^T
4076 ATGCAT
HaeII 84 GGCGCC [GA]GCGC^[CT]
2001 AGCGCT
MluNI 2469 TGGCCA TGG^CCA
2516 TGGCCA
MscI 2469 TGGCCA TGG^CCA
2516 TGGCCA
MvnI 39 CGCG CG^CG
424 CGCG
NsiI 2391 ATGCAT ATGCA^T
4076 ATGCAT
PacI 754 TTAATTAA TTAAT^TAA
1251 TTAATTAA
SpeI 1225 ACTAGT A^CTAGT
3119 ACTAGT
TaqI 219 TCGA T^CGA
1865 TCGA
TthHB8I 219 TCGA T^CGA
1865 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XbaI XcmI
XhoII