##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AccIII AflII AgeI AscI
Asp718I AviII AvrII BamHI BciVI
BfrI BlnI BseAI BsiWI BspDI
BspEI BssHII BstEII BstPI ClaI
CpoI EcoO65I FseI FspI KpnI
KspI MluI MroI NaeI NgoMIV
NheI NotI NruI PinAI PshAI
PvuI RsrII SacII SapI SexAI
SfiI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 943 GACGTC GACGT^C
AclI 4899 AACGTT AA^CGTT
Alw44I 7447 GTGCAC G^TGCAC
ApaLI 7447 GTGCAC G^TGCAC
BbeI 3154 GGCGCC GGCGC^C
BcgI 2882 GCACATTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 8492 TGATCA T^GATCA
BsaWI 7559 TCCGGT [AT]^CCGG[AT]
BsrFI 2437 ACCGGC [GA]^CCGG[CT]
BstBI 7578 TTCGAA TT^CGAA
Cfr10I 2437 ACCGGC [GA]^CCGG[CT]
FbaI 8492 TGATCA T^GATCA
KasI 3154 GGCGCC G^GCGCC
NarI 3154 GGCGCC GG^CGCC
NspV 7578 TTCGAA TT^CGAA
PacI 4064 TTAATTAA TTAAT^TAA
PmeI 8850 GTTTAAAC GTTT^AAAC
Psp1406I 4899 AACGTT AA^CGTT
SalI 9685 GTCGAC G^TCGAC
SbfI 9320 CCTGCAGG CCTGCA^GG
ScaI 4326 AGTACT AGT^ACT
SfoI 3154 GGCGCC GGC^GCC
SfuI 7578 TTCGAA TT^CGAA
Sse8387I 9320 CCTGCAGG CCTGCA^GG
XcmI 905 CCACCACGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 7171 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9291 GACAAGAAGTC
AspEI 7171 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9291 GACAAGAAGTC
BbsI 2902 GTCTTC GAAGAC(2/6)
7093 GTCTTC
BglII 1820 AGATCT A^GATCT
8895 AGATCT
BpuAI 2902 GTCTTC GAAGAC(2/6)
7093 GTCTTC
BsmBI 2084 GAGACG CGTCTC(1/5)
7265 GAGACG
BsrBI 1340 CCGCTC CCGCTC(-3/-3)
6370 GAGCGG
EagI 533 CGGCCG C^GGCCG
2924 CGGCCG
Eam1105I 7171 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
9291 GACAAGAAGTC
EclXI 533 CGGCCG C^GGCCG
2924 CGGCCG
Eco52I 533 CGGCCG C^GGCCG
2924 CGGCCG
EcoRV 5675 GATATC GAT^ATC
8386 GATATC
EcoT22I 2339 ATGCAT ATGCA^T
8933 ATGCAT
HindIII 4535 AAGCTT A^AGCTT
7967 AAGCTT
HpaI 3654 GTTAAC GTT^AAC
8141 GTTAAC
MfeI 2738 CAATTG C^AATTG
6127 CAATTG
MunI 2738 CAATTG C^AATTG
6127 CAATTG
NdeI 842 CATATG CA^TATG
7992 CATATG
NsiI 2339 ATGCAT ATGCA^T
8933 ATGCAT
PaeR7I 160 CTCGAG C^TCGAG
1936 CTCGAG
SmaI 3468 CCCGGG CCC^GGG
7628 CCCGGG
XbaI 3695 TCTAGA T^CTAGA
8892 TCTAGA
XhoI 160 CTCGAG C^TCGAG
1936 CTCGAG
XmaCI 3468 CCCGGG C^CCGGG
7628 CCCGGG
XmaI 3468 CCCGGG C^CCGGG
7628 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 163 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI AseI
AsnI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI Bst1107I
BstAPI BstF5I BstNI BstUI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmnI