##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AscI AseI AsnI Asp718I
BalI BclI BfrI BsaBI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NcoI NdeI
NheI NotI NsiI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 3031 TCCGGA T^CCGGA
AgeI 272 ACCGGT A^CCGGT
AhdI 1201 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 150 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1201 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 78 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1916 TGCGCA TGC^GCA
AvrII 1105 CCTAGG C^CTAGG
BamHI 1502 GGATCC G^GATCC
BbeI 2498 GGCGCC GGCGC^C
BciVI 1410 GTATCC GTATCC(6/5)
BglII 2301 AGATCT A^GATCT
BlnI 1105 CCTAGG C^CTAGG
BseAI 3031 TCCGGA T^CCGGA
BsiEI 837 CGACCG CG[GA][CT]^CG
BspEI 3031 TCCGGA T^CCGGA
BspHI 1514 TCATGA T^CATGA
BsrDI 2269 GCAATG GCAATG(2/0)
DraIII 3546 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1201 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 30 GGCCGGCC GGCCGG^CC
FspI 1916 TGCGCA TGC^GCA
HindIII 3405 AAGCTT A^AGCTT
HpaI 3318 GTTAAC GTT^AAC
KasI 2498 GGCGCC G^GCGCC
MroI 3031 TCCGGA T^CCGGA
NaeI 31 GCCGGC GCC^GGC
NarI 2498 GGCGCC GG^CGCC
NgoMIV 31 GCCGGC G^CCGGC
NruI 2930 TCGCGA TCG^CGA
NspI 1390 GCATGT [GA]CATG^[CT]
PaeR7I 2116 CTCGAG C^TCGAG
PflFI 78 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 272 ACCGGT A^CCGGT
RcaI 1514 TCATGA T^CATGA
SbfI 2178 CCTGCAGG CCTGCA^GG
ScaI 2558 AGTACT AGT^ACT
SfoI 2498 GGCGCC GGC^GCC
Sse8387I 2178 CCTGCAGG CCTGCA^GG
Tth111I 78 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2116 CTCGAG C^TCGAG
XmnI 150 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 112 AAATTC [GA]^AATT[CT]
1242 GAATTT
AocI 361 CCTCAGG CC^T[ACGT]AGG
1801 CCTGAGG
Aor51HI 1862 AGCGCT AGC^GCT
3255 AGCGCT
ApaI 1051 GGGCCC GGGCC^C
3569 GGGCCC
ApoI 112 AAATTC [GA]^AATT[CT]
1242 GAATTT
AvaI 1605 CCCGAG C^[CT]CG[GA]G
2116 CTCGAG
BbvCI 1348 GCTGAGG CCTCAGC(-5/-2)
2190 GCTGAGG
BcgI 270 GCACCGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1859 GCAAGCGCTTCG
BlpI 1 GCTGAGC GC^T[ACGT]AGC
2922 GCTCAGC
Bpu1102I 1 GCTGAGC GC^T[ACGT]AGC
2922 GCTCAGC
BsgI 105 GTGCAG GTGCAG(16/14)
2527 GTGCAG
BsmI 2580 GAATGC GAATGC(1/-1)
3334 GAATGC
BsoBI 1605 CCCGAG C^[CT]CG[GA]G
2116 CTCGAG
BssSI 1786 CACGAG CACGAG(-5/-1)
2098 CACGAG
BstEII 2080 GGTGACC G^GT[ACGT]ACC
2750 GGTCACC
BstPI 2080 GGTGACC G^GT[ACGT]ACC
2750 GGTCACC
Bsu36I 361 CCTCAGG CC^T[ACGT]AGG
1801 CCTGAGG
CelII 1 GCTGAGC GC^T[ACGT]AGC
2922 GCTCAGC
Eco47III 1862 AGCGCT AGC^GCT
3255 AGCGCT
Eco81I 361 CCTCAGG CC^T[ACGT]AGG
1801 CCTGAGG
EcoO65I 2080 GGTGACC G^GT[ACGT]ACC
2750 GGTCACC
SacI 2650 GAGCTC GAGCT^C
2920 GAGCTC
SapI 1184 GAAGAGC GCTCTTC(1/4)
2445 GAAGAGC
SfiI 30 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
412 GGCCGCACGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII