##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AccIII Alw44I ApaLI AscI AseI
AsnI AvrII BclI BglII BlnI
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstZ17I DraI EcoRV EcoT22I
FbaI HpaI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshBI PvuI
SbfI ScaI SfiI SfuI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 8497 GACGTC GACGT^C
Acc65I 5684 GGTACC G^GTACC
AclI 10562 AACGTT AA^CGTT
AflII 5368 CTTAAG C^TTAAG
AhdI 1409 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 5684 GGTACC G^GTACC
AspEI 1409 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1831 GGATCC G^GATCC
BfrI 5368 CTTAAG C^TTAAG
BsmI 7888 GCATTC GAATGC(1/-1)
BspDI 764 ATCGAT AT^CGAT
BssSI 1448 CTCGTG CACGAG(-5/-1)
ClaI 764 ATCGAT AT^CGAT
CpoI 2556 CGGACCG CG^G[AT]CCG
Eam1105I 1409 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5928 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1463 GGCCGGCC GGCCGG^CC
HindIII 8404 AAGCTT A^AGCTT
KpnI 5684 GGTACC GGTAC^C
KspI 5253 CCGCGG CCGC^GG
NdeI 379 CATATG CA^TATG
NheI 6220 GCTAGC G^CTAGC
Psp1406I 10562 AACGTT AA^CGTT
RsrII 2556 CGGACCG CG^G[AT]CCG
SacII 5253 CCGCGG CCGC^GG
SalI 950 GTCGAC G^TCGAC
SapI 4179 GCTCTTC GCTCTTC(1/4)
SgrAI 8032 CACCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AgeI 2580 ACCGGT A^CCGGT
5318 ACCGGT
AocI 9814 CCTGAGG CC^T[ACGT]AGG
10231 CCTGAGG
BciVI 398 GGATAC GTATCC(6/5)
2846 GTATCC
BsaBI 718 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATGCCTATC
BsmBI 2036 GAGACG CGTCTC(1/5)
8290 GAGACG
Bsp1407I 4652 TGTACA T^GTACA
7820 TGTACA
BspHI 1539 TCATGA T^CATGA
7769 TCATGA
BspLU11I 803 ACATGT A^CATGT
7496 ACATGT
BsrGI 4652 TGTACA T^GTACA
7820 TGTACA
BstEII 5707 GGTGACC G^GT[ACGT]ACC
6564 GGTGACC
BstPI 5707 GGTGACC G^GT[ACGT]ACC
6564 GGTGACC
Bsu36I 9814 CCTGAGG CC^T[ACGT]AGG
10231 CCTGAGG
Eco81I 9814 CCTGAGG CC^T[ACGT]AGG
10231 CCTGAGG
EcoO65I 5707 GGTGACC G^GT[ACGT]ACC
6564 GGTGACC
EcoRI 3774 GAATTC G^AATTC
10583 GAATTC
MamI 718 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1019 GATGCCTATC
PinAI 2580 ACCGGT A^CCGGT
5318 ACCGGT
PshAI 3685 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
9336 GACCTGCGTC
RcaI 1539 TCATGA T^CATGA
7769 TCATGA
SacI 194 GAGCTC GAGCT^C
4827 GAGCTC
SphI 645 GCATGC GCATG^C
8966 GCATGC
SspBI 4652 TGTACA T^GTACA
7820 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI