##################################
# NO CUT ENZYMES ( 35 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I BglII BsiWI BspDI BssHII
ClaI CpoI EagI EclXI Eco52I
FseI HpaI KpnI KspI MluI
NdeI NotI NruI PacI PinAI
PmeI PshAI Psp1406I RsrII SacII
SalI ScaI SgrAI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
AviII 191 TGCGCA TGC^GCA
BbrPI 1849 CACGTG CAC^GTG
BsiEI 1031 CGATCG CG[GA][CT]^CG
Bst1107I 9872 GTATAC GTA^TAC
BstZ17I 9872 GTATAC GTA^TAC
EcoRV 97 GATATC GAT^ATC
FspI 191 TGCGCA TGC^GCA
NheI 8939 GCTAGC G^CTAGC
PaeR7I 68 CTCGAG C^TCGAG
PmaCI 1849 CACGTG CAC^GTG
PmlI 1849 CACGTG CAC^GTG
PvuI 1031 CGATCG CGAT^CG
SbfI 2430 CCTGCAGG CCTGCA^GG
SfiI 4579 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5686 CCCGGG CCC^GGG
Sse8387I 2430 CCTGCAGG CCTGCA^GG
XbaI 9116 TCTAGA T^CTAGA
XhoI 68 CTCGAG C^TCGAG
XmaCI 5686 CCCGGG C^CCGGG
XmaI 5686 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 2053 GTGCAC G^TGCAC
7900 GTGCAC
ApaLI 2053 GTGCAC G^TGCAC
7900 GTGCAC
AseI 10227 ATTAAT AT^TAAT
11205 ATTAAT
AsnI 10227 ATTAAT AT^TAAT
11205 ATTAAT
BamHI 794 GGATCC G^GATCC
7380 GGATCC
BbeI 1106 GGCGCC GGCGC^C
1200 GGCGCC
BcgI 3134 GCATCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
9237 GCATGAGGGTCG
BciVI 4597 GGATAC GTATCC(6/5)
11002 GGATAC
BclI 4415 TGATCA T^GATCA
10209 TGATCA
BssSI 5888 CACGAG CACGAG(-5/-1)
4761 CTCGTG
BstBI 26 TTCGAA TT^CGAA
5422 TTCGAA
FbaI 4415 TGATCA T^GATCA
10209 TGATCA
HincII 5566 GTCAAC GT[CT]^[GA]AC
8899 GTCAAC
HindII 5566 GTCAAC GT[CT]^[GA]AC
8899 GTCAAC
HindIII 6853 AAGCTT A^AGCTT
9336 AAGCTT
KasI 1106 GGCGCC G^GCGCC
1200 GGCGCC
NaeI 1109 GCCGGC GCC^GGC
1559 GCCGGC
NarI 1106 GGCGCC GG^CGCC
1200 GGCGCC
NgoMIV 1109 GCCGGC G^CCGGC
1559 GCCGGC
NspV 26 TTCGAA TT^CGAA
5422 TTCGAA
PshBI 10227 ATTAAT AT^TAAT
11205 ATTAAT
SexAI 480 ACCTGGT A^CC[AT]GGT
9421 ACCTGGT
SfoI 1106 GGCGCC GGC^GCC
1200 GGCGCC
SfuI 26 TTCGAA TT^CGAA
5422 TTCGAA
SnaBI 6626 TACGTA TAC^GTA
7806 TACGTA
#########################################
# 3 OR MORE CUT ENZYMES ( 178 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflII AflIII AhdI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI Asp700I AspEI AspHI
AspI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DrdI DsaI
EaeI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI