##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AlwNI AocI Aor51HI AscI
AseI AsnI Asp718I AviII BamHI
BbrPI BcgI BfrI BglI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraII
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HpaI KpnI KspI MamI MluI
MroI NcoI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SpeI SphI Sse8387I SspBI
StuI XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 428 CGCG CG^CG
AclI 2907 AACGTT AA^CGTT
AhdI 3404 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3527 GTGCAC G^TGCAC
ApaI 3035 GGGCCC GGGCC^C
ApaLI 3527 GTGCAC G^TGCAC
AspEI 3404 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2783 CCTAGG C^CTAGG
BbsI 3706 GTCTTC GAAGAC(2/6)
BbvI 1293 GCAGC GCAGC(8/12)
BciVI 2220 GGATAC GTATCC(6/5)
BclI 1233 TGATCA T^GATCA
BglII 94 AGATCT A^GATCT
BlnI 2783 CCTAGG C^CTAGG
BpuAI 3706 GTCTTC GAAGAC(2/6)
BsaAI 3288 TACGTA [CT]AC^GT[GA]
BseRI 2850 CTCCTC GAGGAG(10/8)
BsmFI 2500 GTCCC GGGAC(10/14)
BsrFI 507 GCCGGC [GA]^CCGG[CT]
Bst1107I 1978 GTATAC GTA^TAC
BstUI 428 CGCG CG^CG
BstZ17I 1978 GTATAC GTA^TAC
Cfr10I 507 GCCGGC [GA]^CCGG[CT]
DraIII 1554 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 604 GACCTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3404 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1233 TGATCA T^GATCA
Fnu4HI 1293 GCAGC GC^[ACGT]GC
HindIII 3945 AAGCTT A^AGCTT
ItaI 1293 GCAGC GC^[ACGT]GC
MfeI 3346 CAATTG C^AATTG
MspA1I 100 CAGCTG C[AC]G^C[GT]G
MunI 3346 CAATTG C^AATTG
MvnI 428 CGCG CG^CG
NaeI 507 GCCGGC GCC^GGC
NdeI 1068 CATATG CA^TATG
NgoMIV 507 GCCGGC G^CCGGC
PaeR7I 490 CTCGAG C^TCGAG
PflMI 2724 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2907 AACGTT AA^CGTT
PstI 2225 CTGCAG CTGCA^G
PvuII 100 CAGCTG CAG^CTG
SapI 3082 GAAGAGC GCTCTTC(1/4)
SmlI 490 CTCGAG C^T[CT][GA]AG
SnaBI 3288 TACGTA TAC^GTA
SwaI 197 ATTTAAAT ATTT^AAAT
TaqI 491 TCGA T^CGA
TseI 1293 GCAGC G^C[AT]GC
Tsp45I 3011 GTCAC ^GT[GC]AC
TthHB8I 491 TCGA T^CGA
Van91I 2724 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2206 CCATTCCGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 490 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1978 GTATAC GT^[AC][GT]AC
3471 GTAGAC
AcyI 510 GGCGCC G[GA]^CG[CT]C
2490 GGCGCC
Asp700I 1111 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1436 GAAATTTTTC
AspHI 3151 GTGCTC G[AT]GC[AT]^C
3527 GTGCAC
AspI 1610 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACAGAGTC
AvaII 612 GGTCC G^G[AT]CC
2499 GGTCC
BalI 580 TGGCCA TGG^CCA
1783 TGGCCA
BanII 3035 GGGCCC G[GA]GC[CT]^C
3162 GAGCCC
BbeI 510 GGCGCC GGCGC^C
2490 GGCGCC
BsaHI 510 GGCGCC G[GA]^CG[CT]C
2490 GGCGCC
BsaI 590 GGTCTC GGTCTC(1/5)
1793 GGTCTC
BsgI 2581 CTGCAC GTGCAG(16/14)
1629 GTGCAG
BsiHKAI 3151 GTGCTC G[AT]GC[AT]^C
3527 GTGCAC
BspMI 2983 ACCTGC ACCTGC(4/8)
2881 GCAGGT
BssSI 2400 CACGAG CACGAG(-5/-1)
2973 CTCGTG
BstYI 94 AGATCT [GA]^GATC[CT]
723 AGATCC
HaeII 510 GGCGCC [GA]GCGC^[CT]
2490 GGCGCC
Hin1I 510 GGCGCC G[GA]^CG[CT]C
2490 GGCGCC
KasI 510 GGCGCC G^GCGCC
2490 GGCGCC
MflI 94 AGATCT [GA]^GATC[CT]
723 AGATCC
MluNI 580 TGGCCA TGG^CCA
1783 TGGCCA
MscI 580 TGGCCA TGG^CCA
1783 TGGCCA
NarI 510 GGCGCC GG^CGCC
2490 GGCGCC
NheI 1736 GCTAGC G^CTAGC
2429 GCTAGC
NspI 310 ACATGC [GA]CATG^[CT]
2753 GCATGT
PflFI 1610 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACAGAGTC
SfaNI 3737 GATGC GCATC(5/9)
1049 GCATC
SfoI 510 GGCGCC GGC^GCC
2490 GGCGCC
SmaI 458 CCCGGG CCC^GGG
2542 CCCGGG
Tth111I 1610 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2763 GACAGAGTC
XhoII 94 AGATCT [GA]^GATC[CT]
723 AGATCC
XmaCI 458 CCCGGG C^CCGGG
2542 CCCGGG
XmaI 458 CCCGGG C^CCGGG
2542 CCCGGG
XmnI 1111 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1436 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BanI BbvCI BcnI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmBI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
FauI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfcI SspI StyI
TfiI Tru9I Tsp509I TspRI