##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccII AflII AgeI AhdI ApaI
AscI AspEI AspI AviII BbrPI
BfrI BsiWI BsrBI BsrFI BssHII
BstBI BstEII BstPI BstUI Cfr10I
CpoI DrdI Eam1105I EcoO65I FseI
FspI KspI MluI MvnI NaeI
NgoMIV NheI NruI NspV PflFI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmaI Sse8387I Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1470 GGTACC G^GTACC
AclI 7259 AACGTT AA^CGTT
Aor51HI 164 AGCGCT AGC^GCT
Asp718I 1470 GGTACC G^GTACC
AvaI 779 CTCGAG C^[CT]CG[GA]G
AvrII 826 CCTAGG C^CTAGG
BbeI 79 GGCGCC GGCGC^C
BbvCI 10716 CCTCAGC CCTCAGC(-5/-2)
BglI 10752 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 826 CCTAGG C^CTAGG
BlpI 7396 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7396 GCTAAGC GC^T[ACGT]AGC
BsiEI 10597 CGGCCG CG[GA][CT]^CG
BsoBI 779 CTCGAG C^[CT]CG[GA]G
BspDI 5341 ATCGAT AT^CGAT
BssSI 8186 CTCGTG CACGAG(-5/-1)
Bst1107I 9752 GTATAC GTA^TAC
BstZ17I 9752 GTATAC GTA^TAC
CelII 7396 GCTAAGC GC^T[ACGT]AGC
ClaI 5341 ATCGAT AT^CGAT
DraIII 97 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 10597 CGGCCG C^GGCCG
EclXI 10597 CGGCCG C^GGCCG
Eco47III 164 AGCGCT AGC^GCT
Eco52I 10597 CGGCCG C^GGCCG
FauI 4931 CCCGC CCCGC(4/6)
HgaI 307 GCGTC GACGC(5/10)
HpaI 343 GTTAAC GTT^AAC
KasI 79 GGCGCC G^GCGCC
KpnI 1470 GGTACC GGTAC^C
NarI 79 GGCGCC GG^CGCC
NotI 10596 GCGGCCGC GC^GGCCGC
PacI 9602 TTAATTAA TTAAT^TAA
PaeR7I 779 CTCGAG C^TCGAG
PshAI 8827 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 7259 AACGTT AA^CGTT
SapI 6292 GCTCTTC GCTCTTC(1/4)
SfoI 79 GGCGCC GGC^GCC
SnaBI 5321 TACGTA TAC^GTA
SwaI 530 ATTTAAAT ATTT^AAAT
XbaI 3523 TCTAGA T^CTAGA
XhoI 779 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 5561 GACGTC GACGT^C
9671 GACGTC
AccIII 4922 TCCGGA T^CCGGA
9729 TCCGGA
Alw44I 3239 GTGCAC G^TGCAC
7175 GTGCAC
AocI 5 CCTGAGG CC^T[ACGT]AGG
4197 CCTTAGG
ApaLI 3239 GTGCAC G^TGCAC
7175 GTGCAC
BciVI 5781 GGATAC GTATCC(6/5)
8691 GGATAC
BsaAI 5321 TACGTA [CT]AC^GT[GA]
9755 TACGTG
BseAI 4922 TCCGGA T^CCGGA
9729 TCCGGA
BsmBI 5559 GAGACG CGTCTC(1/5)
9669 GAGACG
BspEI 4922 TCCGGA T^CCGGA
9729 TCCGGA
Bsu36I 5 CCTGAGG CC^T[ACGT]AGG
4197 CCTTAGG
CfoI 80 GCGC GCG^C
165 GCGC
Eco81I 5 CCTGAGG CC^T[ACGT]AGG
4197 CCTTAGG
EcoNI 93 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
11055 CCTAAGATAGG
HaeII 79 GGCGCC [GA]GCGC^[CT]
164 AGCGCT
HhaI 80 GCGC GCG^C
165 GCGC
HinP1I 80 GCGC G^CGC
165 GCGC
HindIII 6924 AAGCTT A^AGCTT
7799 AAGCTT
MroI 4922 TCCGGA T^CCGGA
9729 TCCGGA
PpuMI 3359 GGGTCCT [GA]G^G[AT]CC[CT]
7776 AGGTCCT
SexAI 98 ACCAGGT A^CC[AT]GGT
3213 ACCTGGT
SpeI 5785 ACTAGT A^CTAGT
7230 ACTAGT
StuI 3220 AGGCCT AGG^CCT
4788 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcgI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HapII Hin1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI