################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AccI AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII BciVI BfrI BsaBI BsiWI BspDI BsrDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI MamI MfeI MluI MseI MunI NcoI NdeI NotI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 136 GACGTC GACGT^C Acc65I 1811 GGTACC G^GTACC Aor51HI 706 AGCGCT AGC^GCT Asp718I 1811 GGTACC G^GTACC AvrII 2650 CCTAGG C^CTAGG BamHI 182 GGATCC G^GATCC BbvCI 2565 GCTGAGG CCTCAGC(-5/-2) BclI 2531 TGATCA T^GATCA BlnI 2650 CCTAGG C^CTAGG BlpI 2088 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2088 GCTGAGC GC^T[ACGT]AGC BsiEI 1170 CGGCCG CG[GA][CT]^CG BsmBI 1362 CGTCTC CGTCTC(1/5) BsmI 4338 GAATGC GAATGC(1/-1) Bsp1407I 1493 TGTACA T^GTACA BspHI 156 TCATGA T^CATGA BspLU11I 2840 ACATGT A^CATGT BsrGI 1493 TGTACA T^GTACA BssHII 451 GCGCGC G^CGCGC CelII 2088 GCTGAGC GC^T[ACGT]AGC EagI 1170 CGGCCG C^GGCCG EclXI 1170 CGGCCG C^GGCCG Eco47III 706 AGCGCT AGC^GCT Eco52I 1170 CGGCCG C^GGCCG FbaI 2531 TGATCA T^GATCA FseI 2751 GGCCGGCC GGCCGG^CC KpnI 1811 GGTACC GGTAC^C NheI 3594 GCTAGC G^CTAGC NruI 4203 TCGCGA TCG^CGA PaeR7I 2682 CTCGAG C^TCGAG RcaI 156 TCATGA T^CATGA SapI 1125 GAAGAGC GCTCTTC(1/4) SfiI 1624 GGCCTCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 299 CACCGGCG C[GA]^CCGG[CT]G SspBI 1493 TGTACA T^GTACA XhoI 2682 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1103 ACGTGT A^C[GA][CT]GT 2840 ACATGT BbeI 694 GGCGCC GGCGC^C 1792 GGCGCC BbsI 471 GAAGAC GAAGAC(2/6) 1681 GTCTTC BcgI 1816 CGACTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2069 GCATTGGCATCG BglII 770 AGATCT A^GATCT 1067 AGATCT BpuAI 471 GAAGAC GAAGAC(2/6) 1681 GTCTTC BstAPI 362 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3319 GCAGGCAGTGC DraIII 2743 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3053 CACTTTGTG KasI 694 GGCGCC G^GCGCC 1792 GGCGCC NarI 694 GGCGCC GG^CGCC 1792 GGCGCC SexAI 2332 ACCAGGT A^CC[AT]GGT 3338 ACCTGGT SfoI 694 GGCGCC GGC^GCC 1792 GGCGCC Tsp509I 145 AATT ^AATT 4350 AATT XcmI 1844 CCATCCTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2406 CCAGGTCCAGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI