##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccI AclI AcsI AflII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BciVI
BfrI BsaBI BsiWI BspDI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MseI MunI
NcoI NdeI NotI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 136 GACGTC GACGT^C
Acc65I 1811 GGTACC G^GTACC
Aor51HI 706 AGCGCT AGC^GCT
Asp718I 1811 GGTACC G^GTACC
AvrII 2650 CCTAGG C^CTAGG
BamHI 182 GGATCC G^GATCC
BbvCI 2565 GCTGAGG CCTCAGC(-5/-2)
BclI 2531 TGATCA T^GATCA
BlnI 2650 CCTAGG C^CTAGG
BlpI 2088 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2088 GCTGAGC GC^T[ACGT]AGC
BsiEI 1170 CGGCCG CG[GA][CT]^CG
BsmBI 1362 CGTCTC CGTCTC(1/5)
BsmI 4338 GAATGC GAATGC(1/-1)
Bsp1407I 1493 TGTACA T^GTACA
BspHI 156 TCATGA T^CATGA
BspLU11I 2840 ACATGT A^CATGT
BsrGI 1493 TGTACA T^GTACA
BssHII 451 GCGCGC G^CGCGC
CelII 2088 GCTGAGC GC^T[ACGT]AGC
EagI 1170 CGGCCG C^GGCCG
EclXI 1170 CGGCCG C^GGCCG
Eco47III 706 AGCGCT AGC^GCT
Eco52I 1170 CGGCCG C^GGCCG
FbaI 2531 TGATCA T^GATCA
FseI 2751 GGCCGGCC GGCCGG^CC
KpnI 1811 GGTACC GGTAC^C
NheI 3594 GCTAGC G^CTAGC
NruI 4203 TCGCGA TCG^CGA
PaeR7I 2682 CTCGAG C^TCGAG
RcaI 156 TCATGA T^CATGA
SapI 1125 GAAGAGC GCTCTTC(1/4)
SfiI 1624 GGCCTCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 299 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1493 TGTACA T^GTACA
XhoI 2682 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1103 ACGTGT A^C[GA][CT]GT
2840 ACATGT
BbeI 694 GGCGCC GGCGC^C
1792 GGCGCC
BbsI 471 GAAGAC GAAGAC(2/6)
1681 GTCTTC
BcgI 1816 CGACTGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2069 GCATTGGCATCG
BglII 770 AGATCT A^GATCT
1067 AGATCT
BpuAI 471 GAAGAC GAAGAC(2/6)
1681 GTCTTC
BstAPI 362 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3319 GCAGGCAGTGC
DraIII 2743 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3053 CACTTTGTG
KasI 694 GGCGCC G^GCGCC
1792 GGCGCC
NarI 694 GGCGCC GG^CGCC
1792 GGCGCC
SexAI 2332 ACCAGGT A^CC[AT]GGT
3338 ACCTGGT
SfoI 694 GGCGCC GGC^GCC
1792 GGCGCC
Tsp509I 145 AATT ^AATT
4350 AATT
XcmI 1844 CCATCCTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2406 CCAGGTCCAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI