##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AgeI AscI AvaI
AviII AvrII BbrPI BcgI BglI
BlnI BseAI BsiWI BsoBI BspEI
BsrBI BsrFI BssHII Cfr10I CpoI
DrdI EagI EclXI Eco52I FseI
FspI HpaI KspI MluI MroI
NaeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SbfI SfiI
SgrAI SmaI Sse8387I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 161 GGTACC G^GTACC
AflII 9743 CTTAAG C^TTAAG
AhdI 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 551 GTGCAC G^TGCAC
Aor51HI 2079 AGCGCT AGC^GCT
ApaLI 551 GTGCAC G^TGCAC
Asp718I 161 GGTACC G^GTACC
AspEI 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2002 GGCGCC GGCGC^C
BbvCI 5255 GCTGAGG CCTCAGC(-5/-2)
BciVI 1191 GGATAC GTATCC(6/5)
BcnI 7021 CCCGG CC^[GC]GG
BfrI 9743 CTTAAG C^TTAAG
BlpI 3800 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3800 GCTGAGC GC^T[ACGT]AGC
BsaWI 9151 ACCGGA [AT]^CCGG[AT]
BsiEI 6218 CGACCG CG[GA][CT]^CG
BsmI 5238 GAATGC GAATGC(1/-1)
BssSI 1235 CACGAG CACGAG(-5/-1)
CelII 3800 GCTGAGC GC^T[ACGT]AGC
DraIII 1321 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2079 AGCGCT AGC^GCT
KasI 2002 GGCGCC G^GCGCC
KpnI 161 GGTACC GGTAC^C
NarI 2002 GGCGCC GG^CGCC
NciI 7021 CCCGG CC^[GC]GG
PflFI 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 8262 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 5143 GTCGAC G^TCGAC
SfoI 2002 GGCGCC GGC^GCC
SnaBI 11209 TACGTA TAC^GTA
Tth111I 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2215 CCATGGATTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 2952 CGCG CG^CG
8918 CGCG
AclI 410 AACGTT AA^CGTT
5282 AACGTT
AcyI 2002 GGCGCC G[GA]^CG[CT]C
9497 GACGCC
AocI 3612 CCTGAGG CC^T[ACGT]AGG
3624 CCTGAGG
ApaI 859 GGGCCC GGGCC^C
2117 GGGCCC
BsaBI 4178 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8216 GATCCTCATC
BsaHI 2002 GGCGCC G[GA]^CG[CT]C
9497 GACGCC
BseRI 9082 CTCCTC GAGGAG(10/8)
9315 GAGGAG
BsgI 4002 CTGCAC GTGCAG(16/14)
8959 GTGCAG
BstAPI 5291 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
8710 GCATGATCTGC
BstBI 3771 TTCGAA TT^CGAA
9226 TTCGAA
BstEII 8237 GGTCACC G^GT[ACGT]ACC
9147 GGTGACC
BstPI 8237 GGTCACC G^GT[ACGT]ACC
9147 GGTGACC
BstUI 2952 CGCG CG^CG
8918 CGCG
Bsu36I 3612 CCTGAGG CC^T[ACGT]AGG
3624 CCTGAGG
Eco81I 3612 CCTGAGG CC^T[ACGT]AGG
3624 CCTGAGG
EcoNI 2150 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3226 CCTTTGTTAGG
EcoO65I 8237 GGTCACC G^GT[ACGT]ACC
9147 GGTGACC
FauI 3594 CCCGC CCCGC(4/6)
5305 GCGGG
HaeII 2002 GGCGCC [GA]GCGC^[CT]
2079 AGCGCT
Hin1I 2002 GGCGCC G[GA]^CG[CT]C
9497 GACGCC
MamI 4178 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
8216 GATCCTCATC
MvnI 2952 CGCG CG^CG
8918 CGCG
NheI 5242 GCTAGC G^CTAGC
8759 GCTAGC
NspV 3771 TTCGAA TT^CGAA
9226 TTCGAA
PpuMI 170 AGGACCT [GA]G^G[AT]CC[CT]
5788 AGGTCCT
Psp1406I 410 AACGTT AA^CGTT
5282 AACGTT
SfuI 3771 TTCGAA TT^CGAA
9226 TTCGAA
XbaI 7702 TCTAGA T^CTAGA
7873 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BamHI BanI BanII BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
Bst1107I BstF5I BstNI BstXI BstYI
BstZ17I Cac8I CfoI Cfr13I ClaI
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
SpeI SphI SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI