################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AccIII AgeI AscI AvaI AviII AvrII BbrPI BcgI BglI BlnI BseAI BsiWI BsoBI BspEI BsrBI BsrFI BssHII Cfr10I CpoI DrdI EagI EclXI Eco52I FseI FspI HpaI KspI MluI MroI NaeI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SacII SbfI SfiI SgrAI SmaI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 161 GGTACC G^GTACC AflII 9743 CTTAAG C^TTAAG AhdI 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 551 GTGCAC G^TGCAC Aor51HI 2079 AGCGCT AGC^GCT ApaLI 551 GTGCAC G^TGCAC Asp718I 161 GGTACC G^GTACC AspEI 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 2002 GGCGCC GGCGC^C BbvCI 5255 GCTGAGG CCTCAGC(-5/-2) BciVI 1191 GGATAC GTATCC(6/5) BcnI 7021 CCCGG CC^[GC]GG BfrI 9743 CTTAAG C^TTAAG BlpI 3800 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3800 GCTGAGC GC^T[ACGT]AGC BsaWI 9151 ACCGGA [AT]^CCGG[AT] BsiEI 6218 CGACCG CG[GA][CT]^CG BsmI 5238 GAATGC GAATGC(1/-1) BssSI 1235 CACGAG CACGAG(-5/-1) CelII 3800 GCTGAGC GC^T[ACGT]AGC DraIII 1321 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 9772 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2079 AGCGCT AGC^GCT KasI 2002 GGCGCC G^GCGCC KpnI 161 GGTACC GGTAC^C NarI 2002 GGCGCC GG^CGCC NciI 7021 CCCGG CC^[GC]GG PflFI 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 8262 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 5143 GTCGAC G^TCGAC SfoI 2002 GGCGCC GGC^GCC SnaBI 11209 TACGTA TAC^GTA Tth111I 1704 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2215 CCATGGATTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 2952 CGCG CG^CG 8918 CGCG AclI 410 AACGTT AA^CGTT 5282 AACGTT AcyI 2002 GGCGCC G[GA]^CG[CT]C 9497 GACGCC AocI 3612 CCTGAGG CC^T[ACGT]AGG 3624 CCTGAGG ApaI 859 GGGCCC GGGCC^C 2117 GGGCCC BsaBI 4178 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 8216 GATCCTCATC BsaHI 2002 GGCGCC G[GA]^CG[CT]C 9497 GACGCC BseRI 9082 CTCCTC GAGGAG(10/8) 9315 GAGGAG BsgI 4002 CTGCAC GTGCAG(16/14) 8959 GTGCAG BstAPI 5291 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 8710 GCATGATCTGC BstBI 3771 TTCGAA TT^CGAA 9226 TTCGAA BstEII 8237 GGTCACC G^GT[ACGT]ACC 9147 GGTGACC BstPI 8237 GGTCACC G^GT[ACGT]ACC 9147 GGTGACC BstUI 2952 CGCG CG^CG 8918 CGCG Bsu36I 3612 CCTGAGG CC^T[ACGT]AGG 3624 CCTGAGG Eco81I 3612 CCTGAGG CC^T[ACGT]AGG 3624 CCTGAGG EcoNI 2150 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3226 CCTTTGTTAGG EcoO65I 8237 GGTCACC G^GT[ACGT]ACC 9147 GGTGACC FauI 3594 CCCGC CCCGC(4/6) 5305 GCGGG HaeII 2002 GGCGCC [GA]GCGC^[CT] 2079 AGCGCT Hin1I 2002 GGCGCC G[GA]^CG[CT]C 9497 GACGCC MamI 4178 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 8216 GATCCTCATC MvnI 2952 CGCG CG^CG 8918 CGCG NheI 5242 GCTAGC G^CTAGC 8759 GCTAGC NspV 3771 TTCGAA TT^CGAA 9226 TTCGAA PpuMI 170 AGGACCT [GA]G^G[AT]CC[CT] 5788 AGGTCCT Psp1406I 410 AACGTT AA^CGTT 5282 AACGTT SfuI 3771 TTCGAA TT^CGAA 9226 TTCGAA XbaI 7702 TCTAGA T^CTAGA 7873 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BalI BamHI BanI BanII BbsI BbvI BclI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstXI BstYI BstZ17I Cac8I CfoI Cfr13I ClaI DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SpeI SphI SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI