##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AcyI AgeI AscI AspI
BbeI BciVI BsaHI BsiWI BsrBI
BssHII BstBI DrdI FseI Hin1I
HpaI KasI KspI MluI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 9887 AACGTT AA^CGTT
AhdI 9481 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1604 AGCGCT AGC^GCT
ApaI 425 GGGCCC GGGCC^C
AspEI 9481 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5555 CTCGGG C^[CT]CG[GA]G
AvrII 8114 CCTAGG C^CTAGG
BglI 5475 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 8114 CCTAGG C^CTAGG
BlpI 8711 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 8711 GCTCAGC GC^T[ACGT]AGC
BsaBI 53 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 938 CGGCCG CG[GA][CT]^CG
BsmBI 3435 CGTCTC CGTCTC(1/5)
BsmFI 9479 GGGAC GGGAC(10/14)
BsoBI 5555 CTCGGG C^[CT]CG[GA]G
CelII 8711 GCTCAGC GC^T[ACGT]AGC
CpoI 3050 CGGTCCG CG^G[AT]CCG
EagI 938 CGGCCG C^GGCCG
Eam1105I 9481 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 938 CGGCCG C^GGCCG
Eco47III 1604 AGCGCT AGC^GCT
Eco52I 938 CGGCCG C^GGCCG
MamI 53 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6582 CAATTG C^AATTG
MunI 6582 CAATTG C^AATTG
PflMI 10097 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 8766 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 9887 AACGTT AA^CGTT
RsrII 3050 CGGTCCG CG^G[AT]CCG
SexAI 8930 ACCAGGT A^CC[AT]GGT
SpeI 8301 ACTAGT A^CTAGT
StuI 8955 AGGCCT AGG^CCT
SwaI 10214 ATTTAAAT ATTT^AAAT
Van91I 10097 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2298 GGTACC G^GTACC
4573 GGTACC
AccII 5390 CGCG CG^CG
9136 CGCG
Asp718I 2298 GGTACC G^GTACC
4573 GGTACC
BcgI 5569 GCACGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5604 GCAATGAGATCG
BclI 3351 TGATCA T^GATCA
3827 TGATCA
BspDI 1898 ATCGAT AT^CGAT
5577 ATCGAT
BspHI 6906 TCATGA T^CATGA
8774 TCATGA
Bst1107I 4131 GTATAC GTA^TAC
7186 GTATAC
BstEII 2565 GGTGACC G^GT[ACGT]ACC
8108 GGTAACC
BstPI 2565 GGTGACC G^GT[ACGT]ACC
8108 GGTAACC
BstUI 5390 CGCG CG^CG
9136 CGCG
BstXI 5413 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
9008 CCAAGTCACTGG
BstZ17I 4131 GTATAC GTA^TAC
7186 GTATAC
ClaI 1898 ATCGAT AT^CGAT
5577 ATCGAT
EcoNI 2895 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3891 CCTGCGGAAGG
EcoO65I 2565 GGTGACC G^GT[ACGT]ACC
8108 GGTAACC
EcoRI 7569 GAATTC G^AATTC
10381 GAATTC
FbaI 3351 TGATCA T^GATCA
3827 TGATCA
HincII 3166 GTCAAC GT[CT]^[GA]AC
3947 GTCAAC
HindII 3166 GTCAAC GT[CT]^[GA]AC
3947 GTCAAC
HindIII 87 AAGCTT A^AGCTT
494 AAGCTT
KpnI 2298 GGTACC GGTAC^C
4573 GGTACC
MvnI 5390 CGCG CG^CG
9136 CGCG
NcoI 304 CCATGG C^CATGG
7950 CCATGG
NdeI 8515 CATATG CA^TATG
9878 CATATG
RcaI 6906 TCATGA T^CATGA
8774 TCATGA
SacI 6979 GAGCTC GAGCT^C
7077 GAGCTC
ScaI 1325 AGTACT AGT^ACT
4441 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AflII AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AseI
AsnI Asp700I AspHI AvaII AviII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoRV EcoT14I
EcoT22I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PmaCI
PmlI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI