##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AclI AgeI Aor51HI AscI AviII
BbeI BsiWI BssHII BstBI CpoI
DrdI EagI EclXI Eco47III Eco52I
FseI FspI KasI MluI NarI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SfoI SfuI SgrAI SnaBI
SpeI XhoI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1595 GGTACC G^GTACC
AhdI 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1595 GGTACC G^GTACC
AspEI 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 3982 GGATAC GTATCC(6/5)
Eam1105I 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5115 GATATC GAT^ATC
HpaI 1012 GTTAAC GTT^AAC
KpnI 1595 GGTACC GGTAC^C
KspI 10673 CCGCGG CCGC^GG
NdeI 1156 CATATG CA^TATG
NheI 7560 GCTAGC G^CTAGC
SacII 10673 CCGCGG CCGC^GG
SalI 7185 GTCGAC G^TCGAC
ScaI 5254 AGTACT AGT^ACT
SfiI 8631 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 7793 CCCGGG CCC^GGG
SphI 11003 GCATGC GCATG^C
SwaI 500 ATTTAAAT ATTT^AAAT
XmaCI 7793 CCCGGG C^CCGGG
XmaI 7793 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 3082 GACGTC GACGT^C
5627 GACGTC
AflII 181 CTTAAG C^TTAAG
7572 CTTAAG
AvrII 3978 CCTAGG C^CTAGG
7578 CCTAGG
BfrI 181 CTTAAG C^TTAAG
7572 CTTAAG
BglII 202 AGATCT A^GATCT
6938 AGATCT
BlnI 3978 CCTAGG C^CTAGG
7578 CCTAGG
BsiEI 10271 CGGTCG CG[GA][CT]^CG
10449 CGACCG
BspDI 2368 ATCGAT AT^CGAT
2650 ATCGAT
Bst1107I 474 GTATAC GTA^TAC
6322 GTATAC
BstAPI 3391 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
9144 GCAGTTTTTGC
BstZ17I 474 GTATAC GTA^TAC
6322 GTATAC
ClaI 2368 ATCGAT AT^CGAT
2650 ATCGAT
EcoRI 4646 GAATTC G^AATTC
5123 GAATTC
EcoT22I 723 ATGCAT ATGCA^T
2995 ATGCAT
MfeI 1841 CAATTG C^AATTG
4039 CAATTG
MunI 1841 CAATTG C^AATTG
4039 CAATTG
NaeI 8280 GCCGGC GCC^GGC
8683 GCCGGC
NgoMIV 8280 GCCGGC G^CCGGC
8683 GCCGGC
NsiI 723 ATGCAT ATGCA^T
2995 ATGCAT
SacI 5191 GAGCTC GAGCT^C
10444 GAGCTC
SapI 3276 GAAGAGC GCTCTTC(1/4)
3519 GCTCTTC
SbfI 5730 CCTGCAGG CCTGCA^GG
10907 CCTGCAGG
Sse8387I 5730 CCTGCAGG CCTGCA^GG
10907 CCTGCAGG
SspI 1293 AATATT AAT^ATT
8827 AATATT
XbaI 3727 TCTAGA T^CTAGA
10077 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 175 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AseI AsnI Asp700I AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI