##################################
# NO CUT ENZYMES (   37 enzymes) #
##################################

AclI        AgeI        Aor51HI     AscI        AviII       
BbeI        BsiWI       BssHII      BstBI       CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
FseI        FspI        KasI        MluI        NarI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SfoI        SfuI        SgrAI       SnaBI       
SpeI        XhoI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

Acc65I       1595  GGTACC               G^GTACC
AhdI         9967  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      1595  GGTACC               G^GTACC
AspEI        9967  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI        3982  GGATAC               GTATCC(6/5)
Eam1105I     9967  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        5115  GATATC               GAT^ATC
HpaI         1012  GTTAAC               GTT^AAC
KpnI         1595  GGTACC               GGTAC^C
KspI        10673  CCGCGG               CCGC^GG
NdeI         1156  CATATG               CA^TATG
NheI         7560  GCTAGC               G^CTAGC
SacII       10673  CCGCGG               CCGC^GG
SalI         7185  GTCGAC               G^TCGAC
ScaI         5254  AGTACT               AGT^ACT
SfiI         8631  GGCCGTGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         7793  CCCGGG               CCC^GGG
SphI        11003  GCATGC               GCATG^C
SwaI          500  ATTTAAAT             ATTT^AAAT
XmaCI        7793  CCCGGG               C^CCGGG
XmaI         7793  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AatII        3082  GACGTC               GACGT^C
             5627  GACGTC              
AflII         181  CTTAAG               C^TTAAG
             7572  CTTAAG              
AvrII        3978  CCTAGG               C^CTAGG
             7578  CCTAGG              
BfrI          181  CTTAAG               C^TTAAG
             7572  CTTAAG              
BglII         202  AGATCT               A^GATCT
             6938  AGATCT              
BlnI         3978  CCTAGG               C^CTAGG
             7578  CCTAGG              
BsiEI       10271  CGGTCG               CG[GA][CT]^CG
            10449  CGACCG              
BspDI        2368  ATCGAT               AT^CGAT
             2650  ATCGAT              
Bst1107I      474  GTATAC               GTA^TAC
             6322  GTATAC              
BstAPI       3391  GCAGCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             9144  GCAGTTTTTGC         
BstZ17I       474  GTATAC               GTA^TAC
             6322  GTATAC              
ClaI         2368  ATCGAT               AT^CGAT
             2650  ATCGAT              
EcoRI        4646  GAATTC               G^AATTC
             5123  GAATTC              
EcoT22I       723  ATGCAT               ATGCA^T
             2995  ATGCAT              
MfeI         1841  CAATTG               C^AATTG
             4039  CAATTG              
MunI         1841  CAATTG               C^AATTG
             4039  CAATTG              
NaeI         8280  GCCGGC               GCC^GGC
             8683  GCCGGC              
NgoMIV       8280  GCCGGC               G^CCGGC
             8683  GCCGGC              
NsiI          723  ATGCAT               ATGCA^T
             2995  ATGCAT              
SacI         5191  GAGCTC               GAGCT^C
            10444  GAGCTC              
SapI         3276  GAAGAGC              GCTCTTC(1/4)
             3519  GCTCTTC             
SbfI         5730  CCTGCAGG             CCTGCA^GG
            10907  CCTGCAGG            
Sse8387I     5730  CCTGCAGG             CCTGCA^GG
            10907  CCTGCAGG            
SspI         1293  AATATT               AAT^ATT
             8827  AATATT              
XbaI         3727  TCTAGA               T^CTAGA
            10077  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  175 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcsI        
AcyI        AfaI        AflIII      AluI        Alw44I      
AlwI        AlwNI       AocI        ApaI        ApaLI       
ApoI        AseI        AsnI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbrPI       BbsI        BbvCI       
BbvI        BcgI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PshBI       PstI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XcmI        XhoII       XmnI