################################## # NO CUT ENZYMES ( 37 enzymes) # ################################## AclI AgeI Aor51HI AscI AviII BbeI BsiWI BssHII BstBI CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI KasI MluI NarI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SfoI SfuI SgrAI SnaBI SpeI XhoI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 1595 GGTACC G^GTACC AhdI 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1595 GGTACC G^GTACC AspEI 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 3982 GGATAC GTATCC(6/5) Eam1105I 9967 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 5115 GATATC GAT^ATC HpaI 1012 GTTAAC GTT^AAC KpnI 1595 GGTACC GGTAC^C KspI 10673 CCGCGG CCGC^GG NdeI 1156 CATATG CA^TATG NheI 7560 GCTAGC G^CTAGC SacII 10673 CCGCGG CCGC^GG SalI 7185 GTCGAC G^TCGAC ScaI 5254 AGTACT AGT^ACT SfiI 8631 GGCCGTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 7793 CCCGGG CCC^GGG SphI 11003 GCATGC GCATG^C SwaI 500 ATTTAAAT ATTT^AAAT XmaCI 7793 CCCGGG C^CCGGG XmaI 7793 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 3082 GACGTC GACGT^C 5627 GACGTC AflII 181 CTTAAG C^TTAAG 7572 CTTAAG AvrII 3978 CCTAGG C^CTAGG 7578 CCTAGG BfrI 181 CTTAAG C^TTAAG 7572 CTTAAG BglII 202 AGATCT A^GATCT 6938 AGATCT BlnI 3978 CCTAGG C^CTAGG 7578 CCTAGG BsiEI 10271 CGGTCG CG[GA][CT]^CG 10449 CGACCG BspDI 2368 ATCGAT AT^CGAT 2650 ATCGAT Bst1107I 474 GTATAC GTA^TAC 6322 GTATAC BstAPI 3391 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 9144 GCAGTTTTTGC BstZ17I 474 GTATAC GTA^TAC 6322 GTATAC ClaI 2368 ATCGAT AT^CGAT 2650 ATCGAT EcoRI 4646 GAATTC G^AATTC 5123 GAATTC EcoT22I 723 ATGCAT ATGCA^T 2995 ATGCAT MfeI 1841 CAATTG C^AATTG 4039 CAATTG MunI 1841 CAATTG C^AATTG 4039 CAATTG NaeI 8280 GCCGGC GCC^GGC 8683 GCCGGC NgoMIV 8280 GCCGGC G^CCGGC 8683 GCCGGC NsiI 723 ATGCAT ATGCA^T 2995 ATGCAT SacI 5191 GAGCTC GAGCT^C 10444 GAGCTC SapI 3276 GAAGAGC GCTCTTC(1/4) 3519 GCTCTTC SbfI 5730 CCTGCAGG CCTGCA^GG 10907 CCTGCAGG Sse8387I 5730 CCTGCAGG CCTGCA^GG 10907 CCTGCAGG SspI 1293 AATATT AAT^ATT 8827 AATATT XbaI 3727 TCTAGA T^CTAGA 10077 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 175 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AseI AsnI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI