##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I ApaLI AscI Asp700I Asp718I
AspEI BamHI BbrPI BcgI BclI
BglI BsaAI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI KpnI MamI
MroI NcoI NheI NotI NruI
NsiI NspV PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI StuI SwaI
XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2902 GACGTC GACGT^C
AflII 2583 CTTAAG C^TTAAG
AlwNI 544 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 311 AGCGCT AGC^GCT
ApaI 1075 GGGCCC GGGCC^C
AseI 4147 ATTAAT AT^TAAT
AsnI 4147 ATTAAT AT^TAAT
AspI 2902 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfrI 2583 CTTAAG C^TTAAG
BglII 1838 AGATCT A^GATCT
BsmBI 2755 GAGACG CGTCTC(1/5)
Bsp1407I 2360 TGTACA T^GTACA
BspHI 3231 TCATGA T^CATGA
BspLU11I 3007 ACATGT A^CATGT
BspMI 2892 GCAGGT ACCTGC(4/8)
BsrGI 2360 TGTACA T^GTACA
BstXI 3951 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1035 CGGCCG C^GGCCG
EclXI 1035 CGGCCG C^GGCCG
Eco47III 311 AGCGCT AGC^GCT
Eco52I 1035 CGGCCG C^GGCCG
EcoNI 1015 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 3206 AAGCTT A^AGCTT
HpaI 3568 GTTAAC GTT^AAC
MfeI 3020 CAATTG C^AATTG
MluI 282 ACGCGT A^CGCGT
MunI 3020 CAATTG C^AATTG
NaeI 290 GCCGGC GCC^GGC
NdeI 2045 CATATG CA^TATG
NgoMIV 290 GCCGGC G^CCGGC
NspI 3007 ACATGT [GA]CATG^[CT]
PacI 3176 TTAATTAA TTAAT^TAA
PflFI 2902 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3834 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4147 ATTAAT AT^TAAT
PvuII 2377 CAGCTG CAG^CTG
RcaI 3231 TCATGA T^CATGA
SbfI 2889 CCTGCAGG CCTGCA^GG
SmaI 352 CCCGGG CCC^GGG
Sse8387I 2889 CCTGCAGG CCTGCA^GG
SspBI 2360 TGTACA T^GTACA
Tth111I 2902 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 352 CCCGGG C^CCGGG
XmaI 352 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2071 GTCTAC GT^[AC][GT]AC
2503 GTCTAC
AocI 2251 CCTAAGG CC^T[ACGT]AGG
3301 CCTGAGG
AviII 122 TGCGCA TGC^GCA
1295 TGCGCA
BalI 734 TGGCCA TGG^CCA
4032 TGGCCA
BbvCI 532 CCTCAGC CCTCAGC(-5/-2)
3941 CCTCAGC
BciVI 2229 GGATAC GTATCC(6/5)
3246 GGATAC
BlpI 2866 GCTAAGC GC^T[ACGT]AGC
3319 GCTGAGC
BpmI 2636 CTCCAG CTGGAG(16/14)
3061 CTGGAG
Bpu1102I 2866 GCTAAGC GC^T[ACGT]AGC
3319 GCTGAGC
BsgI 927 CTGCAC GTGCAG(16/14)
2058 CTGCAC
BsrDI 394 CATTGC GCAATG(2/0)
2321 GCAATG
BsrFI 290 GCCGGC [GA]^CCGG[CT]
885 GCCGGT
BstAPI 3103 GCAAGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3905 GCAACCTCTGC
BstEII 858 GGTGACC G^GT[ACGT]ACC
2205 GGTGACC
BstPI 858 GGTGACC G^GT[ACGT]ACC
2205 GGTGACC
Bsu36I 2251 CCTAAGG CC^T[ACGT]AGG
3301 CCTGAGG
CelII 2866 GCTAAGC GC^T[ACGT]AGC
3319 GCTGAGC
Cfr10I 290 GCCGGC [GA]^CCGG[CT]
885 GCCGGT
DrdI 89 GACCCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1322 GACCCCTGGGTC
Eco81I 2251 CCTAAGG CC^T[ACGT]AGG
3301 CCTGAGG
EcoO65I 858 GGTGACC G^GT[ACGT]ACC
2205 GGTGACC
FspI 122 TGCGCA TGC^GCA
1295 TGCGCA
HincII 954 GTTGAC GT[CT]^[GA]AC
3568 GTTAAC
HindII 954 GTTGAC GT[CT]^[GA]AC
3568 GTTAAC
KspI 297 CCGCGG CCGC^GG
901 CCGCGG
MluNI 734 TGGCCA TGG^CCA
4032 TGGCCA
MscI 734 TGGCCA TGG^CCA
4032 TGGCCA
MslI 2719 CACGCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3806 CATATATGTG
PaeR7I 273 CTCGAG C^TCGAG
1144 CTCGAG
PstI 2816 CTGCAG CTGCA^G
2890 CTGCAG
SacI 1138 GAGCTC GAGCT^C
2676 GAGCTC
SacII 297 CCGCGG CCGC^GG
901 CCGCGG
SapI 2620 GAAGAGC GCTCTTC(1/4)
3237 GAAGAGC
SspI 1918 AATATT AAT^ATT
3517 AATATT
XhoI 273 CTCGAG C^TCGAG
1144 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII AvrII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BlnI BmyI BpuAI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII