################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BbrPI BcgI BfrI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SexAI SfiI SfuI SmaI SnaBI SpeI SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 839 GACGTC GACGT^C ApaI 653 GGGCCC GGGCC^C AspI 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2073 TGGCCA TGG^CCA BciVI 2874 GTATCC GTATCC(6/5) BclI 3593 TGATCA T^GATCA BglI 215 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2532 AGATCT A^GATCT BsaAI 2111 TACGTG [CT]AC^GT[GA] BsaI 2667 GAGACC GGTCTC(1/5) BssSI 1700 CTCGTG CACGAG(-5/-1) DraI 3646 TTTAAA TTT^AAA DrdI 3099 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3478 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 854 GAATTC G^AATTC EcoRV 2559 GATATC GAT^ATC FbaI 3593 TGATCA T^GATCA KspI 107 CCGCGG CCGC^GG MluNI 2073 TGGCCA TGG^CCA MscI 2073 TGGCCA TGG^CCA PaeR7I 302 CTCGAG C^TCGAG PflFI 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC SacII 107 CCGCGG CCGC^GG SapI 152 GCTCTTC GCTCTTC(1/4) SbfI 3337 CCTGCAGG CCTGCA^GG ScaI 145 AGTACT AGT^ACT Sse8387I 3337 CCTGCAGG CCTGCA^GG Tth111I 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2166 CCATCGACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 302 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 889 ACGCGT A^C[GA][CT]GT 1689 ACGCGT AgeI 1401 ACCGGT A^CCGGT 1889 ACCGGT AocI 437 CCTCAGG CC^T[ACGT]AGG 862 CCTCAGG AvrII 565 CCTAGG C^CTAGG 2768 CCTAGG BlnI 565 CCTAGG C^CTAGG 2768 CCTAGG BlpI 1360 GCTCAGC GC^T[ACGT]AGC 2098 GCTCAGC Bpu1102I 1360 GCTCAGC GC^T[ACGT]AGC 2098 GCTCAGC BsaWI 1401 ACCGGT [AT]^CCGG[AT] 1889 ACCGGT BsmBI 2674 CGTCTC CGTCTC(1/5) 3434 GAGACG BstXI 342 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1188 CCAACCTTCTGG Bsu36I 437 CCTCAGG CC^T[ACGT]AGG 862 CCTCAGG CelII 1360 GCTCAGC GC^T[ACGT]AGC 2098 GCTCAGC EaeI 99 TGGCCG [CT]^GGCC[GA] 2073 TGGCCA Eco81I 437 CCTCAGG CC^T[ACGT]AGG 862 CCTCAGG HgaI 1630 GACGC GACGC(5/10) 2171 GACGC HincII 495 GTCAAC GT[CT]^[GA]AC 2518 GTTGAC HindII 495 GTCAAC GT[CT]^[GA]AC 2518 GTTGAC MluI 889 ACGCGT A^CGCGT 1689 ACGCGT MslI 1886 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3260 CATCAGGATG NaeI 26 GCCGGC GCC^GGC 1029 GCCGGC NgoMIV 26 GCCGGC G^CCGGC 1029 GCCGGC PflMI 407 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 828 CCATGGTCTGG PinAI 1401 ACCGGT A^CCGGT 1889 ACCGGT PpuMI 568 AGGACCT [GA]G^G[AT]CC[CT] 904 GGGACCC SacI 1099 GAGCTC GAGCT^C 2023 GAGCTC SmlI 302 CTCGAG C^T[CT][GA]AG 2627 CTTGAG Van91I 407 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 828 CCATGGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SgrAI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI