##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII BbrPI BcgI
BfrI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
HindIII HpaI KpnI MamI MfeI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SexAI
SfiI SfuI SmaI SnaBI SpeI
SspBI SspI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 839 GACGTC GACGT^C
ApaI 653 GGGCCC GGGCC^C
AspI 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2073 TGGCCA TGG^CCA
BciVI 2874 GTATCC GTATCC(6/5)
BclI 3593 TGATCA T^GATCA
BglI 215 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2532 AGATCT A^GATCT
BsaAI 2111 TACGTG [CT]AC^GT[GA]
BsaI 2667 GAGACC GGTCTC(1/5)
BssSI 1700 CTCGTG CACGAG(-5/-1)
DraI 3646 TTTAAA TTT^AAA
DrdI 3099 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3478 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 854 GAATTC G^AATTC
EcoRV 2559 GATATC GAT^ATC
FbaI 3593 TGATCA T^GATCA
KspI 107 CCGCGG CCGC^GG
MluNI 2073 TGGCCA TGG^CCA
MscI 2073 TGGCCA TGG^CCA
PaeR7I 302 CTCGAG C^TCGAG
PflFI 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 107 CCGCGG CCGC^GG
SapI 152 GCTCTTC GCTCTTC(1/4)
SbfI 3337 CCTGCAGG CCTGCA^GG
ScaI 145 AGTACT AGT^ACT
Sse8387I 3337 CCTGCAGG CCTGCA^GG
Tth111I 2669 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2166 CCATCGACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 302 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 889 ACGCGT A^C[GA][CT]GT
1689 ACGCGT
AgeI 1401 ACCGGT A^CCGGT
1889 ACCGGT
AocI 437 CCTCAGG CC^T[ACGT]AGG
862 CCTCAGG
AvrII 565 CCTAGG C^CTAGG
2768 CCTAGG
BlnI 565 CCTAGG C^CTAGG
2768 CCTAGG
BlpI 1360 GCTCAGC GC^T[ACGT]AGC
2098 GCTCAGC
Bpu1102I 1360 GCTCAGC GC^T[ACGT]AGC
2098 GCTCAGC
BsaWI 1401 ACCGGT [AT]^CCGG[AT]
1889 ACCGGT
BsmBI 2674 CGTCTC CGTCTC(1/5)
3434 GAGACG
BstXI 342 CCAAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1188 CCAACCTTCTGG
Bsu36I 437 CCTCAGG CC^T[ACGT]AGG
862 CCTCAGG
CelII 1360 GCTCAGC GC^T[ACGT]AGC
2098 GCTCAGC
EaeI 99 TGGCCG [CT]^GGCC[GA]
2073 TGGCCA
Eco81I 437 CCTCAGG CC^T[ACGT]AGG
862 CCTCAGG
HgaI 1630 GACGC GACGC(5/10)
2171 GACGC
HincII 495 GTCAAC GT[CT]^[GA]AC
2518 GTTGAC
HindII 495 GTCAAC GT[CT]^[GA]AC
2518 GTTGAC
MluI 889 ACGCGT A^CGCGT
1689 ACGCGT
MslI 1886 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3260 CATCAGGATG
NaeI 26 GCCGGC GCC^GGC
1029 GCCGGC
NgoMIV 26 GCCGGC G^CCGGC
1029 GCCGGC
PflMI 407 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCATGGTCTGG
PinAI 1401 ACCGGT A^CCGGT
1889 ACCGGT
PpuMI 568 AGGACCT [GA]G^G[AT]CC[CT]
904 GGGACCC
SacI 1099 GAGCTC GAGCT^C
2023 GAGCTC
SmlI 302 CTCGAG C^T[CT][GA]AG
2627 CTTGAG
Van91I 407 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCATGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SgrAI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI