##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AgeI Alw44I ApaI ApaLI AscI
AspI AvrII BlnI BsiWI BspDI
BssHII ClaI CpoI DrdI EagI
EclXI Eco52I EcoRI EcoT22I FseI
KspI MluI NotI NruI NsiI
PacI PflFI PinAI PmeI PvuI
RsrII SacII SalI SfiI SgrAI
SnaBI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 1306 TCCGGA T^CCGGA
AclI 6676 AACGTT AA^CGTT
AhdI 992 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 6880 ATTAAT AT^TAAT
AsnI 6880 ATTAAT AT^TAAT
AspEI 992 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 7386 TGCGCA TGC^GCA
BbeI 4197 GGCGCC GGCGC^C
BbrPI 3472 CACGTG CAC^GTG
BsaWI 1306 TCCGGA [AT]^CCGG[AT]
BseAI 1306 TCCGGA T^CCGGA
BsmBI 376 CGTCTC CGTCTC(1/5)
BspEI 1306 TCCGGA T^CCGGA
BstBI 1119 TTCGAA TT^CGAA
Eam1105I 992 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 7386 TGCGCA TGC^GCA
HgaI 6171 GCGTC GACGC(5/10)
HpaI 410 GTTAAC GTT^AAC
KasI 4197 GGCGCC G^GCGCC
MroI 1306 TCCGGA T^CCGGA
NaeI 1698 GCCGGC GCC^GGC
NarI 4197 GGCGCC GG^CGCC
NdeI 2789 CATATG CA^TATG
NgoMIV 1698 GCCGGC G^CCGGC
NspV 1119 TTCGAA TT^CGAA
PmaCI 3472 CACGTG CAC^GTG
PmlI 3472 CACGTG CAC^GTG
PshAI 9695 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6880 ATTAAT AT^TAAT
Psp1406I 6676 AACGTT AA^CGTT
SacI 6658 GAGCTC GAGCT^C
SapI 1019 GCTCTTC GCTCTTC(1/4)
SbfI 6324 CCTGCAGG CCTGCA^GG
ScaI 6336 AGTACT AGT^ACT
SfoI 4197 GGCGCC GGC^GCC
SfuI 1119 TTCGAA TT^CGAA
SmaI 5227 CCCGGG CCC^GGG
SpeI 8354 ACTAGT A^CTAGT
Sse8387I 6324 CCTGCAGG CCTGCA^GG
XmaCI 5227 CCCGGG C^CCGGG
XmaI 5227 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 4553 GACGTC GACGT^C
9911 GACGTC
Acc65I 9265 GGTACC G^GTACC
11439 GGTACC
AccII 4359 CGCG CG^CG
7568 CGCG
AflII 55 CTTAAG C^TTAAG
3061 CTTAAG
Aor51HI 3156 AGCGCT AGC^GCT
6787 AGCGCT
Asp718I 9265 GGTACC G^GTACC
11439 GGTACC
BamHI 6081 GGATCC G^GATCC
8261 GGATCC
BcgI 6878 CGATTAATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7939 GCATTGCTATCG
BfrI 55 CTTAAG C^TTAAG
3061 CTTAAG
BsaAI 3472 CACGTG [CT]AC^GT[GA]
11084 TACGTG
BsiEI 7405 CGACCG CG[GA][CT]^CG
10052 CGACCG
BsrFI 1698 GCCGGC [GA]^CCGG[CT]
6525 ACCGGC
BssSI 6957 CACGAG CACGAG(-5/-1)
7382 CTCGTG
Bst1107I 1109 GTATAC GTA^TAC
9991 GTATAC
BstUI 4359 CGCG CG^CG
7568 CGCG
BstZ17I 1109 GTATAC GTA^TAC
9991 GTATAC
Cfr10I 1698 GCCGGC [GA]^CCGG[CT]
6525 ACCGGC
DraI 2036 TTTAAA TTT^AAA
6517 TTTAAA
Eco47III 3156 AGCGCT AGC^GCT
6787 AGCGCT
EcoNI 1501 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
11062 CCTGGAGGAGG
HindIII 1999 AAGCTT A^AGCTT
7184 AAGCTT
KpnI 9265 GGTACC GGTAC^C
11439 GGTACC
MvnI 4359 CGCG CG^CG
7568 CGCG
PaeR7I 1446 CTCGAG C^TCGAG
11752 CTCGAG
SexAI 1075 ACCTGGT A^CC[AT]GGT
10312 ACCAGGT
XhoI 1446 CTCGAG C^TCGAG
11752 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI