##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BglI BlnI BlpI
Bpu1102I BseAI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BstBI CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
KpnI KspI MluI MroI NaeI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1070 CGCG CG^CG
AcyI 2968 GGCGCC G[GA]^CG[CT]C
AlwNI 10959 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
BbeI 2968 GGCGCC GGCGC^C
BbrPI 9581 CACGTG CAC^GTG
BcgI 7270 CGATCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 10852 TGATCA T^GATCA
BsaHI 2968 GGCGCC G[GA]^CG[CT]C
BsaWI 9480 ACCGGA [AT]^CCGG[AT]
BsiEI 5325 CGGTCG CG[GA][CT]^CG
BsrBI 8260 GAGCGG CCGCTC(-3/-3)
BssSI 3065 CTCGTG CACGAG(-5/-1)
BstUI 1070 CGCG CG^CG
FbaI 10852 TGATCA T^GATCA
HaeII 2968 GGCGCC [GA]GCGC^[CT]
HgaI 10657 GCGTC GACGC(5/10)
Hin1I 2968 GGCGCC G[GA]^CG[CT]C
KasI 2968 GGCGCC G^GCGCC
MfeI 6162 CAATTG C^AATTG
MunI 6162 CAATTG C^AATTG
MvnI 1070 CGCG CG^CG
NarI 2968 GGCGCC GG^CGCC
NcoI 6523 CCATGG C^CATGG
NheI 4203 GCTAGC G^CTAGC
PaeR7I 2118 CTCGAG C^TCGAG
PflMI 3251 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 9581 CACGTG CAC^GTG
PmlI 9581 CACGTG CAC^GTG
PvuII 6276 CAGCTG CAG^CTG
SfoI 2968 GGCGCC GGC^GCC
Van91I 3251 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2118 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AflII 6139 CTTAAG C^TTAAG
7897 CTTAAG
AocI 1244 CCTCAGG CC^T[ACGT]AGG
2144 CCTGAGG
AseI 962 ATTAAT AT^TAAT
4048 ATTAAT
AsnI 962 ATTAAT AT^TAAT
4048 ATTAAT
AvaI 723 CCCGAG C^[CT]CG[GA]G
2118 CTCGAG
BalI 2439 TGGCCA TGG^CCA
3038 TGGCCA
BfrI 6139 CTTAAG C^TTAAG
7897 CTTAAG
BglII 1960 AGATCT A^GATCT
6250 AGATCT
BsaAI 2380 TACGTG [CT]AC^GT[GA]
9581 CACGTG
BsaBI 1955 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4515 GATTTTAATC
BseRI 11178 CTCCTC GAGGAG(10/8)
8257 GAGGAG
BsoBI 723 CCCGAG C^[CT]CG[GA]G
2118 CTCGAG
BsrDI 3634 CATTGC GCAATG(2/0)
1063 GCAATG
Bst1107I 1945 GTATAC GTA^TAC
6595 GTATAC
BstEII 6518 GGTTACC G^GT[ACGT]ACC
10844 GGTCACC
BstPI 6518 GGTTACC G^GT[ACGT]ACC
10844 GGTCACC
BstYI 1960 AGATCT [GA]^GATC[CT]
6250 AGATCT
BstZ17I 1945 GTATAC GTA^TAC
6595 GTATAC
Bsu36I 1244 CCTCAGG CC^T[ACGT]AGG
2144 CCTGAGG
CfoI 1069 GCGC GCG^C
2969 GCGC
DraIII 3743 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
8109 CACTCCGTG
DrdI 260 GACTATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1674 GACTACACAGTC
DsaI 759 CCGTGG C^C[GA][CT]GG
6523 CCATGG
EaeI 2439 TGGCCA [CT]^GGCC[GA]
3038 TGGCCA
Eco81I 1244 CCTCAGG CC^T[ACGT]AGG
2144 CCTGAGG
EcoNI 4440 CCTTGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
10872 CCTCCCACAGG
EcoO65I 6518 GGTTACC G^GT[ACGT]ACC
10844 GGTCACC
EcoRV 3211 GATATC GAT^ATC
6642 GATATC
HhaI 1069 GCGC GCG^C
2969 GCGC
HinP1I 1069 GCGC G^CGC
2969 GCGC
HpaI 5091 GTTAAC GTT^AAC
10989 GTTAAC
MamI 1955 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4515 GATTTTAATC
MflI 1960 AGATCT [GA]^GATC[CT]
6250 AGATCT
MluNI 2439 TGGCCA TGG^CCA
3038 TGGCCA
MscI 2439 TGGCCA TGG^CCA
3038 TGGCCA
MspA1I 6276 CAGCTG C[AC]G^C[GT]G
8036 CCGCTG
NdeI 4731 CATATG CA^TATG
6316 CATATG
PpuMI 727 AGGTCCT [GA]G^G[AT]CC[CT]
2148 AGGACCT
PshBI 962 ATTAAT AT^TAAT
4048 ATTAAT
PstI 8022 CTGCAG CTGCA^G
8269 CTGCAG
SacI 5742 GAGCTC GAGCT^C
6094 GAGCTC
StuI 7333 AGGCCT AGG^CCT
7891 AGGCCT
XhoII 1960 AGATCT [GA]^GATC[CT]
6250 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XmnI