##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII BamHI BbeI BbrPI BbvCI
BglI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspHI BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HaeII HpaI
KasI KpnI KspI MamI MroI
NarI NcoI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmlI PshAI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 5 CGCG CG^CG
AclI 4610 AACGTT AA^CGTT
AcyI 2182 GACGCC G[GA]^CG[CT]C
Asp700I 4781 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1015 CCTAGG C^CTAGG
BalI 604 TGGCCA TGG^CCA
BanI 5499 GGTGCC G^G[CT][GA]CC
BanII 450 GAGCCC G[GA]GC[CT]^C
BcgI 13 CGAGTTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3063 GTATCC GTATCC(6/5)
BglII 3223 AGATCT A^GATCT
BlnI 1015 CCTAGG C^CTAGG
BsaHI 2182 GACGCC G[GA]^CG[CT]C
BsmBI 2332 CGTCTC CGTCTC(1/5)
BsmI 3391 GAATGC GAATGC(1/-1)
BspLU11I 1038 ACATGT A^CATGT
BsrFI 3528 GCCGGC [GA]^CCGG[CT]
BssSI 867 CACGAG CACGAG(-5/-1)
BstAPI 4494 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 5 CGCG CG^CG
CfoI 48 GCGC GCG^C
Cfr10I 3528 GCCGGC [GA]^CCGG[CT]
EaeI 604 TGGCCA [CT]^GGCC[GA]
EcoT22I 2319 ATGCAT ATGCA^T
FauI 1271 CCCGC CCCGC(4/6)
HhaI 48 GCGC GCG^C
Hin1I 2182 GACGCC G[GA]^CG[CT]C
HinP1I 48 GCGC G^CGC
MluI 4 ACGCGT A^CGCGT
MluNI 604 TGGCCA TGG^CCA
MscI 604 TGGCCA TGG^CCA
MvnI 5 CGCG CG^CG
NaeI 3528 GCCGGC GCC^GGC
NdeI 3029 CATATG CA^TATG
NgoMIV 3528 GCCGGC G^CCGGC
NheI 907 GCTAGC G^CTAGC
NsiI 2319 ATGCAT ATGCA^T
PmeI 5549 GTTTAAAC GTTT^AAAC
PpuMI 5469 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 4610 AACGTT AA^CGTT
SphI 414 GCATGC GCATG^C
SspI 947 AATATT AAT^ATT
StuI 1398 AGGCCT AGG^CCT
XcmI 1164 CCAGTCAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4781 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 747 GTAGAC GT^[AC][GT]AC
4367 GTAGAC
AflII 5106 CTTAAG C^TTAAG
5474 CTTAAG
AspHI 705 GTGCTC G[AT]GC[AT]^C
4213 GAGCAC
BfrI 5106 CTTAAG C^TTAAG
5474 CTTAAG
BsiHKAI 705 GTGCTC G[AT]GC[AT]^C
4213 GAGCAC
Bsp1407I 242 TGTACA T^GTACA
1280 TGTACA
BsrDI 2617 CATTGC GCAATG(2/0)
4386 CATTGC
BsrGI 242 TGTACA T^GTACA
1280 TGTACA
DraII 3984 AGGCCCC [GA]G^G[ACGT]CC[CT]
5469 AGGTCCT
DraIII 1515 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2640 CACCATGTG
EcoO109I 3984 AGGCCCC [GA]G^G[ACGT]CC[CT]
5469 AGGTCCT
MfeI 1002 CAATTG C^AATTG
5594 CAATTG
MunI 1002 CAATTG C^AATTG
5594 CAATTG
PflMI 2947 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCATAAGATGG
PstI 2586 CTGCAG CTGCA^G
4066 CTGCAG
ScaI 4877 AGTACT AGT^ACT
5628 AGTACT
SmlI 5106 CTTAAG C^T[CT][GA]AG
5474 CTTAAG
SpeI 935 ACTAGT A^CTAGT
2476 ACTAGT
SspBI 242 TGTACA T^GTACA
1280 TGTACA
Van91I 2947 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCATAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AvaII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoNI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
SwaI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII