##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI Aor51HI ApaI AscI AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BglI BlnI BsaAI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII Bst1107I BstUI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
HaeII KasI KspI MluI MvnI
NarI NotI NruI NsiI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 512 GGTACC G^GTACC
AccI 4990 GTCTAC GT^[AC][GT]AC
AccIII 2896 TCCGGA T^CCGGA
AcyI 676 GGCGTC G[GA]^CG[CT]C
Alw44I 6073 GTGCAC G^TGCAC
AocI 1785 CCTGAGG CC^T[ACGT]AGG
ApaLI 6073 GTGCAC G^TGCAC
Asp718I 512 GGTACC G^GTACC
AspI 2205 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 985 TGGCCA TGG^CCA
BsaHI 676 GGCGTC G[GA]^CG[CT]C
BsaI 1899 GAGACC GGTCTC(1/5)
BseAI 2896 TCCGGA T^CCGGA
BspEI 2896 TCCGGA T^CCGGA
BsrFI 1594 GCCGGC [GA]^CCGG[CT]
BssSI 2334 CACGAG CACGAG(-5/-1)
BstBI 3247 TTCGAA TT^CGAA
BstEII 609 GGTGACC G^GT[ACGT]ACC
BstPI 609 GGTGACC G^GT[ACGT]ACC
BstXI 976 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1785 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1594 GCCGGC [GA]^CCGG[CT]
Eco81I 1785 CCTGAGG CC^T[ACGT]AGG
EcoO65I 609 GGTGACC G^GT[ACGT]ACC
EcoRI 775 GAATTC G^AATTC
FauI 4202 CCCGC CCCGC(4/6)
Hin1I 676 GGCGTC G[GA]^CG[CT]C
HpaI 4339 GTTAAC GTT^AAC
KpnI 512 GGTACC GGTAC^C
MfeI 4369 CAATTG C^AATTG
MluNI 985 TGGCCA TGG^CCA
MroI 2896 TCCGGA T^CCGGA
MscI 985 TGGCCA TGG^CCA
MunI 4369 CAATTG C^AATTG
NaeI 1594 GCCGGC GCC^GGC
NdeI 4638 CATATG CA^TATG
NgoMIV 1594 GCCGGC G^CCGGC
NspV 3247 TTCGAA TT^CGAA
PaeR7I 2031 CTCGAG C^TCGAG
PflFI 2205 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 108 GAAGAGC GCTCTTC(1/4)
SfuI 3247 TTCGAA TT^CGAA
SmaI 3727 CCCGGG CCC^GGG
StuI 3671 AGGCCT AGG^CCT
Tth111I 2205 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4929 TCTAGA T^CTAGA
XhoI 2031 CTCGAG C^TCGAG
XmaCI 3727 CCCGGG C^CCGGG
XmaI 3727 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaI 2031 CTCGAG C^[CT]CG[GA]G
3727 CCCGGG
BamHI 1980 GGATCC G^GATCC
2686 GGATCC
BanI 512 GGTACC G^G[CT][GA]CC
1601 GGCACC
BlpI 882 GCTGAGC GC^T[ACGT]AGC
3735 GCTGAGC
Bpu1102I 882 GCTGAGC GC^T[ACGT]AGC
3735 GCTGAGC
BsmI 1123 GCATTC GAATGC(1/-1)
2125 GCATTC
BsoBI 2031 CTCGAG C^[CT]CG[GA]G
3727 CCCGGG
BspHI 1693 TCATGA T^CATGA
4153 TCATGA
BspLU11I 4398 ACATGT A^CATGT
5075 ACATGT
BstAPI 2493 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3018 GCAGAAACTGC
CelII 882 GCTGAGC GC^T[ACGT]AGC
3735 GCTGAGC
DraIII 3003 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
5873 CACCGTGTG
EaeI 985 TGGCCA [CT]^GGCC[GA]
2194 TGGCCG
PacI 5998 TTAATTAA TTAAT^TAA
6002 TTAATTAA
PshAI 2238 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3933 GACAGCAGTC
RcaI 1693 TCATGA T^CATGA
4153 TCATGA
ScaI 1698 AGTACT AGT^ACT
6749 AGTACT
XcmI 1933 CCACGGATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2231 CCACAGGGACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI