##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AgeI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
AvrII BamHI BbrPI BglI BlnI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EarI
Eco47III EcoO65I FseI FspI KpnI
Ksp632I KspI MluI MroI MspA1I
MvnI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI Sse8387I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 2443 AACGTT AA^CGTT
AflII 1166 CTTAAG C^TTAAG
AhdI 836 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 836 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4239 GAGCAC G[AT]GC[AT]^C
BanII 3327 GAGCCC G[GA]GC[CT]^C
BbeI 5590 GGCGCC GGCGC^C
BcgI 5684 GCACCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1166 CTTAAG C^TTAAG
BglII 3235 AGATCT A^GATCT
BlpI 691 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 691 GCTGAGC GC^T[ACGT]AGC
BsiEI 3924 CGGCCG CG[GA][CT]^CG
BsiHKAI 4239 GAGCAC G[AT]GC[AT]^C
BssSI 2646 CTCGTG CACGAG(-5/-1)
CelII 691 GCTGAGC GC^T[ACGT]AGC
EagI 3924 CGGCCG C^GGCCG
Eam1105I 836 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3924 CGGCCG C^GGCCG
Eco52I 3924 CGGCCG C^GGCCG
EcoNI 661 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 5882 GATATC GAT^ATC
HaeII 5590 GGCGCC [GA]GCGC^[CT]
KasI 5590 GGCGCC G^GCGCC
MfeI 5408 CAATTG C^AATTG
MunI 5408 CAATTG C^AATTG
NarI 5590 GGCGCC GG^CGCC
NdeI 371 CATATG CA^TATG
Psp1406I 2443 AACGTT AA^CGTT
PstI 494 CTGCAG CTGCA^G
SfoI 5590 GGCGCC GGC^GCC
SmaI 4280 CCCGGG CCC^GGG
SnaBI 298 TACGTA TAC^GTA
XbaI 162 TCTAGA T^CTAGA
XmaCI 4280 CCCGGG C^CCGGG
XmaI 4280 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 3881 GGCGTC G[GA]^CG[CT]C
5590 GGCGCC
BbsI 2638 GAAGAC GAAGAC(2/6)
4206 GAAGAC
BpuAI 2638 GAAGAC GAAGAC(2/6)
4206 GAAGAC
BsaBI 535 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5804 GATGATCATC
BsaHI 3881 GGCGTC G[GA]^CG[CT]C
5590 GGCGCC
BsgI 3680 GTGCAG GTGCAG(16/14)
5361 GTGCAG
Bsp1407I 401 TGTACA T^GTACA
6023 TGTACA
BsrDI 2784 CATTGC GCAATG(2/0)
5521 CATTGC
BsrGI 401 TGTACA T^GTACA
6023 TGTACA
BstAPI 4267 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5354 GCAAATAGTGC
DraII 2217 AGGACCT [GA]G^G[ACGT]CC[CT]
2768 AGGTCCC
DsaI 1860 CCATGG C^C[GA][CT]GG
2832 CCATGG
EcoO109I 2217 AGGACCT [GA]G^G[ACGT]CC[CT]
2768 AGGTCCC
FauI 5594 CCCGC CCCGC(4/6)
5687 CCCGC
HapII 3923 CCGG C^CGG
4281 CCGG
HgaI 643 GCGTC GACGC(5/10)
3882 GCGTC
Hin1I 3881 GGCGTC G[GA]^CG[CT]C
5590 GGCGCC
HpaI 3493 GTTAAC GTT^AAC
5041 GTTAAC
HpaII 3923 CCGG C^CGG
4281 CCGG
MamI 535 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5804 GATGATCATC
MspI 3923 CCGG C^CGG
4281 CCGG
NcoI 1860 CCATGG C^CATGG
2832 CCATGG
NheI 4096 GCTAGC G^CTAGC
5957 GCTAGC
PpuMI 2217 AGGACCT [GA]G^G[AT]CC[CT]
2768 AGGTCCC
ScaI 5873 AGTACT AGT^ACT
5918 AGTACT
SphI 2138 GCATGC GCATG^C
2311 GCATGC
SspBI 401 TGTACA T^GTACA
6023 TGTACA
StuI 1866 AGGCCT AGG^CCT
4331 AGGCCT
SwaI 1713 ATTTAAAT ATTT^AAAT
5335 ATTTAAAT
XcmI 2199 CCAACCATTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2673 CCATCTAGCAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AseI AsnI
Asp700I AvaI AvaII BalI BanI
BbvCI BbvI BciVI BclI BcnI
BfaI BmyI BpmI BsaAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII EaeI Eco81I EcoRI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HindIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI