##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AflII AgeI AscI AvrII
BciVI BfrI BlnI Bsp1407I BspDI
BspLU11I BsrGI BssHII ClaI DraI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FseI HpaI MfeI MluI
MunI NdeI NheI NotI NsiI
PacI PaeR7I PinAI PmeI Psp1406I
PvuI SalI SfiI SgrAI SnaBI
SpeI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1464 GACGTC GACGT^C
Acc65I 802 GGTACC G^GTACC
AccI 2125 GTATAC GT^[AC][GT]AC
Alw44I 1555 GTGCAC G^TGCAC
ApaLI 1555 GTGCAC G^TGCAC
AseI 2185 ATTAAT AT^TAAT
AsnI 2185 ATTAAT AT^TAAT
Asp718I 802 GGTACC G^GTACC
AviII 3491 TGCGCA TGC^GCA
BbeI 4893 GGCGCC GGCGC^C
BclI 6062 TGATCA T^GATCA
BglI 2326 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 192 GAGACC GGTCTC(1/5)
BsiEI 6155 CGGTCG CG[GA][CT]^CG
BsiWI 3194 CGTACG C^GTACG
Bst1107I 2125 GTATAC GTA^TAC
BstBI 734 TTCGAA TT^CGAA
BstZ17I 2125 GTATAC GTA^TAC
CpoI 2581 CGGTCCG CG^G[AT]CCG
FbaI 6062 TGATCA T^GATCA
FspI 3491 TGCGCA TGC^GCA
HindIII 220 AAGCTT A^AGCTT
KasI 4893 GGCGCC G^GCGCC
KpnI 802 GGTACC GGTAC^C
KspI 41 CCGCGG CCGC^GG
NarI 4893 GGCGCC GG^CGCC
NspV 734 TTCGAA TT^CGAA
PshAI 3601 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2185 ATTAAT AT^TAAT
RsrII 2581 CGGTCCG CG^G[AT]CCG
SacII 41 CCGCGG CCGC^GG
SbfI 3294 CCTGCAGG CCTGCA^GG
SfoI 4893 GGCGCC GGC^GCC
SfuI 734 TTCGAA TT^CGAA
Sse8387I 3294 CCTGCAGG CCTGCA^GG
XbaI 5145 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 3713 TCCGGA T^CCGGA
4995 TCCGGA
AflIII 3191 ACACGT A^C[GA][CT]GT
5285 ACGTGT
AhdI 786 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5419 GACTACAAGTC
AocI 5037 CCTCAGG CC^T[ACGT]AGG
5395 CCTGAGG
Aor51HI 3121 AGCGCT AGC^GCT
4031 AGCGCT
ApaI 2319 GGGCCC GGGCC^C
4096 GGGCCC
AspEI 786 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5419 GACTACAAGTC
BamHI 492 GGATCC G^GATCC
1325 GGATCC
BbrPI 4081 CACGTG CAC^GTG
6512 CACGTG
BbvCI 3613 CCTCAGC CCTCAGC(-5/-2)
6303 CCTCAGC
BsaBI 618 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2782 GATCCTGATC
BseAI 3713 TCCGGA T^CCGGA
4995 TCCGGA
BsmI 1368 GAATGC GAATGC(1/-1)
1686 GCATTC
BspEI 3713 TCCGGA T^CCGGA
4995 TCCGGA
BssSI 1281 CACGAG CACGAG(-5/-1)
47 CTCGTG
BstEII 2622 GGTCACC G^GT[ACGT]ACC
4836 GGTCACC
BstPI 2622 GGTCACC G^GT[ACGT]ACC
4836 GGTCACC
Bsu36I 5037 CCTCAGG CC^T[ACGT]AGG
5395 CCTGAGG
Eam1105I 786 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5419 GACTACAAGTC
Eco47III 3121 AGCGCT AGC^GCT
4031 AGCGCT
Eco81I 5037 CCTCAGG CC^T[ACGT]AGG
5395 CCTGAGG
EcoNI 3249 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4234 CCTACTCAAGG
EcoO65I 2622 GGTCACC G^GT[ACGT]ACC
4836 GGTCACC
HincII 1695 GTCAAC GT[CT]^[GA]AC
3905 GTCAAC
HindII 1695 GTCAAC GT[CT]^[GA]AC
3905 GTCAAC
MamI 618 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2782 GATCCTGATC
MroI 3713 TCCGGA T^CCGGA
4995 TCCGGA
NaeI 27 GCCGGC GCC^GGC
5621 GCCGGC
NgoMIV 27 GCCGGC G^CCGGC
5621 GCCGGC
NruI 628 TCGCGA TCG^CGA
4179 TCGCGA
PmaCI 4081 CACGTG CAC^GTG
6512 CACGTG
PmlI 4081 CACGTG CAC^GTG
6512 CACGTG
SmlI 1935 CTTGAG C^T[CT][GA]AG
4238 CTCAAG
SphI 863 GCATGC GCATG^C
4137 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI