##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AhdI AscI AseI AsnI AspEI
AviII BbvCI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstZ17I ClaI CpoI Eam1105I
EcoRI EcoT22I FseI FspI MamI
MluI NheI NotI NruI NsiI
PacI PaeR7I PmeI PshBI PvuI
RcaI RsrII SalI ScaI SexAI
SfiI SphI SspBI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 4892 GACGTC GACGT^C
Acc65I 3780 GGTACC G^GTACC
AccI 1176 GTAGAC GT^[AC][GT]AC
AclI 4499 AACGTT AA^CGTT
AflII 4658 CTTAAG C^TTAAG
AgeI 3348 ACCGGT A^CCGGT
Aor51HI 2091 AGCGCT AGC^GCT
ApaI 4599 GGGCCC GGGCC^C
Asp718I 3780 GGTACC G^GTACC
AvrII 4370 CCTAGG C^CTAGG
BamHI 2353 GGATCC G^GATCC
BfrI 4658 CTTAAG C^TTAAG
BlnI 4370 CCTAGG C^CTAGG
BsrBI 4567 CCGCTC CCGCTC(-3/-3)
BsrDI 2289 GCAATG GCAATG(2/0)
BssHII 86 GCGCGC G^CGCGC
BssSI 1269 CACGAG CACGAG(-5/-1)
BstBI 4014 TTCGAA TT^CGAA
BstEII 408 GGTGACC G^GT[ACGT]ACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
BstYI 2353 GGATCC [GA]^GATC[CT]
DraIII 6149 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2691 GACTCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2091 AGCGCT AGC^GCT
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
EcoRV 2912 GATATC GAT^ATC
KpnI 3780 GGTACC GGTAC^C
KspI 295 CCGCGG CCGC^GG
MfeI 4759 CAATTG C^AATTG
MflI 2353 GGATCC [GA]^GATC[CT]
MunI 4759 CAATTG C^AATTG
NcoI 172 CCATGG C^CATGG
NdeI 2841 CATATG CA^TATG
NspV 4014 TTCGAA TT^CGAA
PinAI 3348 ACCGGT A^CCGGT
PshAI 1614 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4499 AACGTT AA^CGTT
SacII 295 CCGCGG CCGC^GG
SbfI 4509 CCTGCAGG CCTGCA^GG
SfuI 4014 TTCGAA TT^CGAA
SgrAI 3347 CACCGGTG C[GA]^CCGG[CT]G
SmaI 3052 CCCGGG CCC^GGG
SnaBI 4861 TACGTA TAC^GTA
Sse8387I 4509 CCTGCAGG CCTGCA^GG
SspI 6569 AATATT AAT^ATT
SwaI 6559 ATTTAAAT ATTT^AAAT
XhoII 2353 GGATCC [GA]^GATC[CT]
XmaCI 3052 CCCGGG C^CCGGG
XmaI 3052 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1587 TCCGGA T^CCGGA
1835 TCCGGA
AflIII 1578 ACGTGT A^C[GA][CT]GT
1902 ACGTGT
Alw44I 4516 GTGCAC G^TGCAC
5427 GTGCAC
ApaLI 4516 GTGCAC G^TGCAC
5427 GTGCAC
Asp700I 612 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6210 GAAATTTTTC
BalI 419 TGGCCA TGG^CCA
947 TGGCCA
BbeI 177 GGCGCC GGCGC^C
281 GGCGCC
BbrPI 1901 CACGTG CAC^GTG
4917 CACGTG
BclI 5132 TGATCA T^GATCA
6066 TGATCA
BglI 273 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4592 GCCCACAGGGC
BseAI 1587 TCCGGA T^CCGGA
1835 TCCGGA
BspEI 1587 TCCGGA T^CCGGA
1835 TCCGGA
EagI 271 CGGCCG C^GGCCG
2704 CGGCCG
EclXI 271 CGGCCG C^GGCCG
2704 CGGCCG
Eco52I 271 CGGCCG C^GGCCG
2704 CGGCCG
FbaI 5132 TGATCA T^GATCA
6066 TGATCA
HpaI 2966 GTTAAC GTT^AAC
3197 GTTAAC
KasI 177 GGCGCC G^GCGCC
281 GGCGCC
MluNI 419 TGGCCA TGG^CCA
947 TGGCCA
MroI 1587 TCCGGA T^CCGGA
1835 TCCGGA
MscI 419 TGGCCA TGG^CCA
947 TGGCCA
NarI 177 GGCGCC GG^CGCC
281 GGCGCC
NspI 5948 ACATGC [GA]CATG^[CT]
5981 GCATGT
PflMI 950 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3302 CCAGGCAGTGG
PmaCI 1901 CACGTG CAC^GTG
4917 CACGTG
PmlI 1901 CACGTG CAC^GTG
4917 CACGTG
SfoI 177 GGCGCC GGC^GCC
281 GGCGCC
SpeI 1026 ACTAGT A^CTAGT
5249 ACTAGT
StuI 3831 AGGCCT AGG^CCT
4981 AGGCCT
Van91I 950 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3302 CCAGGCAGTGG
XbaI 3899 TCTAGA T^CTAGA
5673 TCTAGA
XmnI 612 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6210 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AspI AvaI AvaII BanI
BanII BbsI BbvI BcgI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I