##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AgeI ApoI AscI AseI AsnI
BamHI BciVI BclI BglII BsaBI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EcoO65I EcoRI EcoT22I FbaI
FseI MamI MfeI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SalI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 5013 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5013 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1512 CCTAGG C^CTAGG
BbeI 6229 GGCGCC GGCGC^C
BcgI 6167 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1512 CCTAGG C^CTAGG
Bsp1407I 901 TGTACA T^GTACA
BspHI 1481 TCATGA T^CATGA
BsrGI 901 TGTACA T^GTACA
BssHII 505 GCGCGC G^CGCGC
Eam1105I 5013 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 4819 GATATC GAT^ATC
HindIII 1327 AAGCTT A^AGCTT
HpaI 3646 GTTAAC GTT^AAC
KasI 6229 GGCGCC G^GCGCC
KspI 3406 CCGCGG CCGC^GG
MluI 1159 ACGCGT A^CGCGT
NarI 6229 GGCGCC GG^CGCC
PshAI 5292 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1481 TCATGA T^CATGA
SacII 3406 CCGCGG CCGC^GG
SbfI 4464 CCTGCAGG CCTGCA^GG
SfoI 6229 GGCGCC GGC^GCC
SphI 3623 GCATGC GCATG^C
Sse8387I 4464 CCTGCAGG CCTGCA^GG
SspBI 901 TGTACA T^GTACA
XbaI 2697 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 616 GGTACC G^GTACC
976 GGTACC
AflII 4380 CTTAAG C^TTAAG
4728 CTTAAG
AflIII 1159 ACGCGT A^C[GA][CT]GT
2039 ACGTGT
Alw44I 5367 GTGCAC G^TGCAC
6194 GTGCAC
ApaLI 5367 GTGCAC G^TGCAC
6194 GTGCAC
Asp700I 574 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1729 GAACCTCTTC
Asp718I 616 GGTACC G^GTACC
976 GGTACC
AviII 559 TGCGCA TGC^GCA
2117 TGCGCA
BbrPI 2038 CACGTG CAC^GTG
5628 CACGTG
BbsI 999 GAAGAC GAAGAC(2/6)
5675 GAAGAC
BfrI 4380 CTTAAG C^TTAAG
4728 CTTAAG
BpuAI 999 GAAGAC GAAGAC(2/6)
5675 GAAGAC
BsaAI 2038 CACGTG [CT]AC^GT[GA]
5628 CACGTG
BsiEI 3235 CGGCCG CG[GA][CT]^CG
6177 CGGCCG
BsrBI 194 CCGCTC CCGCTC(-3/-3)
2023 GAGCGG
BstYI 2988 AGATCC [GA]^GATC[CT]
5945 GGATCT
EagI 3235 CGGCCG C^GGCCG
6177 CGGCCG
EclXI 3235 CGGCCG C^GGCCG
6177 CGGCCG
Eco52I 3235 CGGCCG C^GGCCG
6177 CGGCCG
FspI 559 TGCGCA TGC^GCA
2117 TGCGCA
KpnI 616 GGTACC GGTAC^C
976 GGTACC
MflI 2988 AGATCC [GA]^GATC[CT]
5945 GGATCT
PmaCI 2038 CACGTG CAC^GTG
5628 CACGTG
PmlI 2038 CACGTG CAC^GTG
5628 CACGTG
XcmI 3359 CCATCCGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4262 CCATGCCCAGCCTGG
XhoII 2988 AGATCC [GA]^GATC[CT]
5945 GGATCT
XmnI 574 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1729 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XmaCI XmaI