##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AclI AhdI Alw44I ApaLI
AseI AsnI AspEI BbrPI BsaBI
BsiWI Bsp1407I BspDI BspHI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I HpaI MamI MfeI MluI
MunI NdeI NspV PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII ScaI SfuI SnaBI
SpeI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AflII 7435 CTTAAG C^TTAAG
AgeI 2288 ACCGGT A^CCGGT
AscI 94 GGCGCGCC GG^CGCGCC
AspI 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1939 CCTAGG C^CTAGG
BfrI 7435 CTTAAG C^TTAAG
BglII 5525 AGATCT A^GATCT
BlnI 1939 CCTAGG C^CTAGG
BsaAI 8821 TACGTG [CT]AC^GT[GA]
BspLU11I 3915 ACATGT A^CATGT
BssHII 95 GCGCGC G^CGCGC
DraI 3663 TTTAAA TTT^AAA
EcoRV 5541 GATATC GAT^ATC
EcoT22I 2421 ATGCAT ATGCA^T
NheI 8814 GCTAGC G^CTAGC
NsiI 2421 ATGCAT ATGCA^T
PflFI 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2288 ACCGGT A^CCGGT
SgrAI 8326 CGCCGGCG C[GA]^CCGG[CT]G
Tth111I 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 9384 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 4204 GTCGAC GT^[AC][GT]AC
8214 GTCGAC
AcsI 7834 GAATTC [GA]^AATT[CT]
8512 GAATTC
AflIII 446 ACACGT A^C[GA][CT]GT
3915 ACATGT
ApoI 7834 GAATTC [GA]^AATT[CT]
8512 GAATTC
AviII 6392 TGCGCA TGC^GCA
7466 TGCGCA
BciVI 3494 GTATCC GTATCC(6/5)
7016 GTATCC
BclI 1596 TGATCA T^GATCA
5312 TGATCA
BsrDI 9087 CATTGC GCAATG(2/0)
5713 GCAATG
DrdI 3510 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6835 GACCAGGGGGTC
EcoRI 7834 GAATTC G^AATTC
8512 GAATTC
FbaI 1596 TGATCA T^GATCA
5312 TGATCA
FseI 50 GGCCGGCC GGCCGG^CC
818 GGCCGGCC
FspI 6392 TGCGCA TGC^GCA
7466 TGCGCA
HindIII 3889 AAGCTT A^AGCTT
6515 AAGCTT
MseI 3664 TTAA T^TAA
7436 TTAA
NruI 1031 TCGCGA TCG^CGA
3127 TCGCGA
PaeR7I 723 CTCGAG C^TCGAG
8168 CTCGAG
PshAI 721 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1315 GACGAGGGTC
SacI 4171 GAGCTC GAGCT^C
4834 GAGCTC
SalI 4204 GTCGAC G^TCGAC
8214 GTCGAC
SbfI 2793 CCTGCAGG CCTGCA^GG
2837 CCTGCAGG
SphI 4271 GCATGC GCATG^C
8996 GCATGC
Sse8387I 2793 CCTGCAGG CCTGCA^GG
2837 CCTGCAGG
Tru9I 3664 TTAA T^TAA
7436 TTAA
XhoI 723 CTCGAG C^TCGAG
8168 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 168 enzymes) #
#########################################
Acc65I AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI Asp700I Asp718I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NotI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacII SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI