##################################
# NO CUT ENZYMES (   44 enzymes) #
##################################

AatII       AclI        AhdI        Alw44I      ApaLI       
AseI        AsnI        AspEI       BbrPI       BsaBI       
BsiWI       Bsp1407I    BspDI       BspHI       BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
Eam1105I    HpaI        MamI        MfeI        MluI        
MunI        NdeI        NspV        PacI        PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       ScaI        SfuI        SnaBI       
SpeI        SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AflII        7435  CTTAAG               C^TTAAG
AgeI         2288  ACCGGT               A^CCGGT
AscI           94  GGCGCGCC             GG^CGCGCC
AspI         7105  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1939  CCTAGG               C^CTAGG
BfrI         7435  CTTAAG               C^TTAAG
BglII        5525  AGATCT               A^GATCT
BlnI         1939  CCTAGG               C^CTAGG
BsaAI        8821  TACGTG               [CT]AC^GT[GA]
BspLU11I     3915  ACATGT               A^CATGT
BssHII         95  GCGCGC               G^CGCGC
DraI         3663  TTTAAA               TTT^AAA
EcoRV        5541  GATATC               GAT^ATC
EcoT22I      2421  ATGCAT               ATGCA^T
NheI         8814  GCTAGC               G^CTAGC
NsiI         2421  ATGCAT               ATGCA^T
PflFI        7105  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2288  ACCGGT               A^CCGGT
SgrAI        8326  CGCCGGCG             C[GA]^CCGG[CT]G
Tth111I      7105  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         9384  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         4204  GTCGAC               GT^[AC][GT]AC
             8214  GTCGAC              
AcsI         7834  GAATTC               [GA]^AATT[CT]
             8512  GAATTC              
AflIII        446  ACACGT               A^C[GA][CT]GT
             3915  ACATGT              
ApoI         7834  GAATTC               [GA]^AATT[CT]
             8512  GAATTC              
AviII        6392  TGCGCA               TGC^GCA
             7466  TGCGCA              
BciVI        3494  GTATCC               GTATCC(6/5)
             7016  GTATCC              
BclI         1596  TGATCA               T^GATCA
             5312  TGATCA              
BsrDI        9087  CATTGC               GCAATG(2/0)
             5713  GCAATG              
DrdI         3510  GACATGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6835  GACCAGGGGGTC        
EcoRI        7834  GAATTC               G^AATTC
             8512  GAATTC              
FbaI         1596  TGATCA               T^GATCA
             5312  TGATCA              
FseI           50  GGCCGGCC             GGCCGG^CC
              818  GGCCGGCC            
FspI         6392  TGCGCA               TGC^GCA
             7466  TGCGCA              
HindIII      3889  AAGCTT               A^AGCTT
             6515  AAGCTT              
MseI         3664  TTAA                 T^TAA
             7436  TTAA                
NruI         1031  TCGCGA               TCG^CGA
             3127  TCGCGA              
PaeR7I        723  CTCGAG               C^TCGAG
             8168  CTCGAG              
PshAI         721  GACTCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1315  GACGAGGGTC          
SacI         4171  GAGCTC               GAGCT^C
             4834  GAGCTC              
SalI         4204  GTCGAC               G^TCGAC
             8214  GTCGAC              
SbfI         2793  CCTGCAGG             CCTGCA^GG
             2837  CCTGCAGG            
SphI         4271  GCATGC               GCATG^C
             8996  GCATGC              
Sse8387I     2793  CCTGCAGG             CCTGCA^GG
             2837  CCTGCAGG            
Tru9I        3664  TTAA                 T^TAA
             7436  TTAA                
XhoI          723  CTCGAG               C^TCGAG
             8168  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  168 enzymes) #
#########################################

Acc65I      AccII       AccIII      AciI        AcyI        
AfaI        AluI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        Asp700I     Asp718I     AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspEI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NotI        NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        XmnI