##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AcsI AflII ApoI
AscI AseI AsnI AspI AviII
BclI BfrI BsaBI BseAI BsiWI
BspEI BspHI BspLU11I BsrBI BssHII
DraI EarI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
Ksp632I MamI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PmeI PshBI
Psp1406I RcaI SalI SapI ScaI
SgrAI SnaBI SpeI SspI SwaI
Tsp509I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 3730 GACGTC GACGT^C
AccI 4194 GTATAC GT^[AC][GT]AC
AflIII 5411 ACGTGT A^C[GA][CT]GT
AgeI 4989 ACCGGT A^CCGGT
AhdI 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 266 AGCGCT AGC^GCT
Asp700I 4015 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglII 441 AGATCT A^GATCT
BsrDI 4652 CATTGC GCAATG(2/0)
Bst1107I 4194 GTATAC GTA^TAC
BstBI 4215 TTCGAA TT^CGAA
BstZ17I 4194 GTATAC GTA^TAC
CpoI 4167 CGGACCG CG^G[AT]CCG
DrdI 4707 GACTTAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
HincII 4716 GTCAAC GT[CT]^[GA]AC
HindII 4716 GTCAAC GT[CT]^[GA]AC
MseI 4710 TTAA T^TAA
NheI 2583 GCTAGC G^CTAGC
NspV 4215 TTCGAA TT^CGAA
PinAI 4989 ACCGGT A^CCGGT
PshAI 4862 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4963 CGATCG CGAT^CG
RsrII 4167 CGGACCG CG^G[AT]CCG
SfiI 673 GGCCTCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4215 TTCGAA TT^CGAA
TfiI 4015 GAATC G^A[AT]TC
Tru9I 4710 TTAA T^TAA
XmnI 4015 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BamHI 3161 GGATCC G^GATCC
5040 GGATCC
BcgI 54 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4953 GCAGGGTCCTCG
BciVI 3617 GGATAC GTATCC(6/5)
1670 GTATCC
BsmBI 226 CGTCTC CGTCTC(1/5)
5786 CGTCTC
BsmI 1375 GAATGC GAATGC(1/-1)
4142 GAATGC
Bsp1407I 1743 TGTACA T^GTACA
4816 TGTACA
BspDI 503 ATCGAT AT^CGAT
575 ATCGAT
BsrGI 1743 TGTACA T^GTACA
4816 TGTACA
BssSI 182 CTCGTG CACGAG(-5/-1)
3832 CTCGTG
BstEII 1928 GGTGACC G^GT[ACGT]ACC
3237 GGTCACC
BstPI 1928 GGTGACC G^GT[ACGT]ACC
3237 GGTCACC
ClaI 503 ATCGAT AT^CGAT
575 ATCGAT
DraIII 1249 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4257 CACGACGTG
EcoO65I 1928 GGTGACC G^GT[ACGT]ACC
3237 GGTCACC
SacI 4446 GAGCTC GAGCT^C
5299 GAGCTC
SbfI 3693 CCTGCAGG CCTGCA^GG
5562 CCTGCAGG
SphI 3882 GCATGC GCATG^C
5330 GCATGC
Sse8387I 3693 CCTGCAGG CCTGCA^GG
5562 CCTGCAGG
SspBI 1743 TGTACA T^GTACA
4816 TGTACA
XbaI 1794 TCTAGA T^CTAGA
5936 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp718I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KpnI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI