################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccIII AclI AcsI AflII ApoI AscI AseI AsnI AspI AviII BclI BfrI BsaBI BseAI BsiWI BspEI BspHI BspLU11I BsrBI BssHII DraI EarI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI PacI PaeR7I PflFI PmeI PshBI Psp1406I RcaI SalI SapI ScaI SgrAI SnaBI SpeI SspI SwaI Tsp509I Tth111I XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 3730 GACGTC GACGT^C AccI 4194 GTATAC GT^[AC][GT]AC AflIII 5411 ACGTGT A^C[GA][CT]GT AgeI 4989 ACCGGT A^CCGGT AhdI 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 266 AGCGCT AGC^GCT Asp700I 4015 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BglII 441 AGATCT A^GATCT BsrDI 4652 CATTGC GCAATG(2/0) Bst1107I 4194 GTATAC GTA^TAC BstBI 4215 TTCGAA TT^CGAA BstZ17I 4194 GTATAC GTA^TAC CpoI 4167 CGGACCG CG^G[AT]CCG DrdI 4707 GACTTAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1258 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 266 AGCGCT AGC^GCT HincII 4716 GTCAAC GT[CT]^[GA]AC HindII 4716 GTCAAC GT[CT]^[GA]AC MseI 4710 TTAA T^TAA NheI 2583 GCTAGC G^CTAGC NspV 4215 TTCGAA TT^CGAA PinAI 4989 ACCGGT A^CCGGT PshAI 4862 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 4963 CGATCG CGAT^CG RsrII 4167 CGGACCG CG^G[AT]CCG SfiI 673 GGCCTCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 4215 TTCGAA TT^CGAA TfiI 4015 GAATC G^A[AT]TC Tru9I 4710 TTAA T^TAA XmnI 4015 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BamHI 3161 GGATCC G^GATCC 5040 GGATCC BcgI 54 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4953 GCAGGGTCCTCG BciVI 3617 GGATAC GTATCC(6/5) 1670 GTATCC BsmBI 226 CGTCTC CGTCTC(1/5) 5786 CGTCTC BsmI 1375 GAATGC GAATGC(1/-1) 4142 GAATGC Bsp1407I 1743 TGTACA T^GTACA 4816 TGTACA BspDI 503 ATCGAT AT^CGAT 575 ATCGAT BsrGI 1743 TGTACA T^GTACA 4816 TGTACA BssSI 182 CTCGTG CACGAG(-5/-1) 3832 CTCGTG BstEII 1928 GGTGACC G^GT[ACGT]ACC 3237 GGTCACC BstPI 1928 GGTGACC G^GT[ACGT]ACC 3237 GGTCACC ClaI 503 ATCGAT AT^CGAT 575 ATCGAT DraIII 1249 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4257 CACGACGTG EcoO65I 1928 GGTGACC G^GT[ACGT]ACC 3237 GGTCACC SacI 4446 GAGCTC GAGCT^C 5299 GAGCTC SbfI 3693 CCTGCAGG CCTGCA^GG 5562 CCTGCAGG SphI 3882 GCATGC GCATG^C 5330 GCATGC Sse8387I 3693 CCTGCAGG CCTGCA^GG 5562 CCTGCAGG SspBI 1743 TGTACA T^GTACA 4816 TGTACA XbaI 1794 TCTAGA T^CTAGA 5936 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp718I AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI