################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI AscI AseI AsnI Asp718I AviII BamHI BbrPI BlpI Bpu1102I BsaAI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BstBI BstEII BstPI CelII ClaI CpoI DraIII Eco47III EcoNI EcoO65I EcoRV EcoT22I FseI FspI HpaI KpnI MluI MroI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AatII 4522 GACGTC GACGT^C AccI 692 GTATAC GT^[AC][GT]AC AflII 1894 CTTAAG C^TTAAG Alw44I 3366 GTGCAC G^TGCAC ApaLI 3366 GTGCAC G^TGCAC Asp700I 4630 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 1576 GCAAACGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 724 TGATCA T^GATCA BfrI 1894 CTTAAG C^TTAAG BsaBI 422 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 4481 ACATGT A^CATGT Bst1107I 692 GTATAC GTA^TAC BstZ17I 692 GTATAC GTA^TAC DraI 1758 TTTAAA TTT^AAA FbaI 724 TGATCA T^GATCA HindIII 2422 AAGCTT A^AGCTT MamI 422 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 3258 CCATGG C^CATGG PflFI 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 853 ACCAGGT A^CC[AT]GGT SfiI 3176 GGCCTGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tth111I 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3828 TCTAGA T^CTAGA XmnI 4630 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 4481 ACATGT A^C[GA][CT]GT 5264 ACACGT BbeI 314 GGCGCC GGCGC^C 384 GGCGCC BbvCI 5374 CCTCAGC CCTCAGC(-5/-2) 4366 GCTGAGG BciVI 3167 GGATAC GTATCC(6/5) 4300 GGATAC BglII 842 AGATCT A^GATCT 1878 AGATCT BsaWI 178 ACCGGA [AT]^CCGG[AT] 1247 TCCGGT BsiEI 195 CGGCCG CG[GA][CT]^CG 269 CGGCCG BsmI 4630 GAATGC GAATGC(1/-1) 1153 GCATTC BspHI 613 TCATGA T^CATGA 4518 TCATGA BstAPI 1519 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3498 GCAGTAGGTGC DrdI 2079 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3247 GACTGGGGTGTC EagI 195 CGGCCG C^GGCCG 269 CGGCCG EclXI 195 CGGCCG C^GGCCG 269 CGGCCG Eco52I 195 CGGCCG C^GGCCG 269 CGGCCG EcoRI 4257 GAATTC G^AATTC 4428 GAATTC KasI 314 GGCGCC G^GCGCC 384 GGCGCC KspI 133 CCGCGG CCGC^GG 322 CCGCGG MfeI 3281 CAATTG C^AATTG 4273 CAATTG MunI 3281 CAATTG C^AATTG 4273 CAATTG NarI 314 GGCGCC GG^CGCC 384 GGCGCC NdeI 2240 CATATG CA^TATG 4768 CATATG PstI 714 CTGCAG CTGCA^G 3625 CTGCAG RcaI 613 TCATGA T^CATGA 4518 TCATGA SacI 1468 GAGCTC GAGCT^C 4175 GAGCTC SacII 133 CCGCGG CCGC^GG 322 CCGCGG SfoI 314 GGCGCC GGC^GCC 384 GGCGCC SspI 2188 AATATT AAT^ATT 4788 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApaI ApoI AspEI AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI