##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
BamHI BbrPI BlpI Bpu1102I BsaAI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII BstBI BstEII BstPI
CelII ClaI CpoI DraIII Eco47III
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HpaI KpnI MluI MroI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 4522 GACGTC GACGT^C
AccI 692 GTATAC GT^[AC][GT]AC
AflII 1894 CTTAAG C^TTAAG
Alw44I 3366 GTGCAC G^TGCAC
ApaLI 3366 GTGCAC G^TGCAC
Asp700I 4630 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1576 GCAAACGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 724 TGATCA T^GATCA
BfrI 1894 CTTAAG C^TTAAG
BsaBI 422 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4481 ACATGT A^CATGT
Bst1107I 692 GTATAC GTA^TAC
BstZ17I 692 GTATAC GTA^TAC
DraI 1758 TTTAAA TTT^AAA
FbaI 724 TGATCA T^GATCA
HindIII 2422 AAGCTT A^AGCTT
MamI 422 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3258 CCATGG C^CATGG
PflFI 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 853 ACCAGGT A^CC[AT]GGT
SfiI 3176 GGCCTGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 4025 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3828 TCTAGA T^CTAGA
XmnI 4630 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 4481 ACATGT A^C[GA][CT]GT
5264 ACACGT
BbeI 314 GGCGCC GGCGC^C
384 GGCGCC
BbvCI 5374 CCTCAGC CCTCAGC(-5/-2)
4366 GCTGAGG
BciVI 3167 GGATAC GTATCC(6/5)
4300 GGATAC
BglII 842 AGATCT A^GATCT
1878 AGATCT
BsaWI 178 ACCGGA [AT]^CCGG[AT]
1247 TCCGGT
BsiEI 195 CGGCCG CG[GA][CT]^CG
269 CGGCCG
BsmI 4630 GAATGC GAATGC(1/-1)
1153 GCATTC
BspHI 613 TCATGA T^CATGA
4518 TCATGA
BstAPI 1519 GCACTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3498 GCAGTAGGTGC
DrdI 2079 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3247 GACTGGGGTGTC
EagI 195 CGGCCG C^GGCCG
269 CGGCCG
EclXI 195 CGGCCG C^GGCCG
269 CGGCCG
Eco52I 195 CGGCCG C^GGCCG
269 CGGCCG
EcoRI 4257 GAATTC G^AATTC
4428 GAATTC
KasI 314 GGCGCC G^GCGCC
384 GGCGCC
KspI 133 CCGCGG CCGC^GG
322 CCGCGG
MfeI 3281 CAATTG C^AATTG
4273 CAATTG
MunI 3281 CAATTG C^AATTG
4273 CAATTG
NarI 314 GGCGCC GG^CGCC
384 GGCGCC
NdeI 2240 CATATG CA^TATG
4768 CATATG
PstI 714 CTGCAG CTGCA^G
3625 CTGCAG
RcaI 613 TCATGA T^CATGA
4518 TCATGA
SacI 1468 GAGCTC GAGCT^C
4175 GAGCTC
SacII 133 CCGCGG CCGC^GG
322 CCGCGG
SfoI 314 GGCGCC GGC^GCC
384 GGCGCC
SspI 2188 AATATT AAT^ATT
4788 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI AocI
ApaI ApoI AspEI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI