##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbrPI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsiWI BspDI BspHI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI Eam1105I EcoRV
EcoT22I FbaI HincII HindII HpaI
KpnI MluI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 1283 TCCGGA T^CCGGA
AcsI 1351 GAATTC [GA]^AATT[CT]
AcyI 4628 GGCGCC G[GA]^CG[CT]C
Alw44I 2194 GTGCAC G^TGCAC
Aor51HI 840 AGCGCT AGC^GCT
ApaLI 2194 GTGCAC G^TGCAC
ApoI 1351 GAATTC [GA]^AATT[CT]
AspI 3148 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4628 GGCGCC GGCGC^C
BsaBI 1580 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4628 GGCGCC G[GA]^CG[CT]C
BsaWI 1283 TCCGGA [AT]^CCGG[AT]
BseAI 1283 TCCGGA T^CCGGA
BspEI 1283 TCCGGA T^CCGGA
BsrFI 5099 GCCGGC [GA]^CCGG[CT]
BssSI 2118 CACGAG CACGAG(-5/-1)
BstEII 1879 GGTCACC G^GT[ACGT]ACC
BstPI 1879 GGTCACC G^GT[ACGT]ACC
Cfr10I 5099 GCCGGC [GA]^CCGG[CT]
DraIII 3871 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 840 AGCGCT AGC^GCT
EcoO65I 1879 GGTCACC G^GT[ACGT]ACC
EcoRI 1351 GAATTC G^AATTC
FseI 5098 GGCCGGCC GGCCGG^CC
Hin1I 4628 GGCGCC G[GA]^CG[CT]C
HindIII 1145 AAGCTT A^AGCTT
KasI 4628 GGCGCC G^GCGCC
MamI 1580 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1387 CAATTG C^AATTG
MroI 1283 TCCGGA T^CCGGA
MunI 1387 CAATTG C^AATTG
NaeI 5099 GCCGGC GCC^GGC
NarI 4628 GGCGCC GG^CGCC
NdeI 1065 CATATG CA^TATG
NgoMIV 5099 GCCGGC G^CCGGC
PflFI 3148 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 4628 GGCGCC GGC^GCC
SpeI 610 ACTAGT A^CTAGT
Tth111I 3148 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1510 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 2628 ACATGT A^C[GA][CT]GT
2749 ACATGT
BbsI 1897 GAAGAC GAAGAC(2/6)
2975 GTCTTC
BglI 1089 GCCTACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3513 GCCCAAGAGGC
BglII 1579 AGATCT A^GATCT
5084 AGATCT
BpuAI 1897 GAAGAC GAAGAC(2/6)
2975 GTCTTC
BsaI 3631 GAGACC GGTCTC(1/5)
2386 GGTCTC
BsiEI 4795 CGGCCG CG[GA][CT]^CG
4864 CGGCCG
BsmBI 1828 CGTCTC CGTCTC(1/5)
3463 CGTCTC
BsmI 457 GAATGC GAATGC(1/-1)
1113 GCATTC
Bsp1407I 330 TGTACA T^GTACA
2111 TGTACA
BspLU11I 2628 ACATGT A^CATGT
2749 ACATGT
BsrBI 5184 CCGCTC CCGCTC(-3/-3)
5338 GAGCGG
BsrDI 3817 CATTGC GCAATG(2/0)
2573 GCAATG
BsrGI 330 TGTACA T^GTACA
2111 TGTACA
EagI 4795 CGGCCG C^GGCCG
4864 CGGCCG
EclXI 4795 CGGCCG C^GGCCG
4864 CGGCCG
Eco52I 4795 CGGCCG C^GGCCG
4864 CGGCCG
KspI 3529 CCGCGG CCGC^GG
4618 CCGCGG
MaeII 3152 ACGT A^CGT
4262 ACGT
PaeR7I 3844 CTCGAG C^TCGAG
4038 CTCGAG
PshAI 2836 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4386 GACTCAGGTC
SacII 3529 CCGCGG CCGC^GG
4618 CCGCGG
SexAI 3236 ACCAGGT A^CC[AT]GGT
3872 ACCTGGT
SspBI 330 TGTACA T^GTACA
2111 TGTACA
XhoI 3844 CTCGAG C^TCGAG
4038 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvCI BbvI BcgI BciVI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI