##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AspI AviII
BlpI Bpu1102I BsaWI BseAI BsiWI
BspDI BspEI BstEII BstPI CelII
ClaI CpoI DrdI EagI EclXI
Eco52I EcoO65I FseI FspI KpnI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SexAI SfiI
SnaBI SpeI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 6258 CTTAAG C^TTAAG
AhdI 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5464 GTGCAC G^TGCAC
ApaLI 5464 GTGCAC G^TGCAC
AspEI 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 4591 GGCGCC GGCGC^C
BbrPI 3356 CACGTG CAC^GTG
BcgI 4440 GCAGGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3017 GTATCC GTATCC(6/5)
BclI 2093 TGATCA T^GATCA
BfrI 6258 CTTAAG C^TTAAG
BsaBI 1366 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2107 GAGACC GGTCTC(1/5)
BsiEI 4943 CGACCG CG[GA][CT]^CG
Bsp1407I 1709 TGTACA T^GTACA
BspHI 1641 TCATGA T^CATGA
BspLU11I 5898 ACATGT A^CATGT
BsrGI 1709 TGTACA T^GTACA
BssHII 4506 GCGCGC G^CGCGC
BssSI 4034 CACGAG CACGAG(-5/-1)
BstBI 3960 TTCGAA TT^CGAA
DraI 5386 TTTAAA TTT^AAA
DraIII 5065 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2724 CCTACTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2093 TGATCA T^GATCA
HpaI 5699 GTTAAC GTT^AAC
KasI 4591 GGCGCC G^GCGCC
KspI 4727 CCGCGG CCGC^GG
MamI 1366 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4591 GGCGCC GG^CGCC
NcoI 4668 CCATGG C^CATGG
NspV 3960 TTCGAA TT^CGAA
PaeR7I 3797 CTCGAG C^TCGAG
PmaCI 3356 CACGTG CAC^GTG
PmlI 3356 CACGTG CAC^GTG
PshAI 4971 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1641 TCATGA T^CATGA
SacI 4933 GAGCTC GAGCT^C
SacII 4727 CCGCGG CCGC^GG
SalI 4941 GTCGAC G^TCGAC
ScaI 4574 AGTACT AGT^ACT
SfoI 4591 GGCGCC GGC^GCC
SfuI 3960 TTCGAA TT^CGAA
SgrAI 94 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1709 TGTACA T^GTACA
XhoI 3797 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AatII 3375 GACGTC GACGT^C
3733 GACGTC
AflIII 3355 ACACGT A^C[GA][CT]GT
5898 ACATGT
BamHI 4291 GGATCC G^GATCC
4745 GGATCC
BsaAI 1586 TACGTG [CT]AC^GT[GA]
3356 CACGTG
BsmBI 2662 CGTCTC CGTCTC(1/5)
4969 GAGACG
BsrFI 95 ACCGGC [GA]^CCGG[CT]
1308 ACCGGC
Bst1107I 2888 GTATAC GTA^TAC
4120 GTATAC
BstZ17I 2888 GTATAC GTA^TAC
4120 GTATAC
Cfr10I 95 ACCGGC [GA]^CCGG[CT]
1308 ACCGGC
EcoRI 1075 GAATTC G^AATTC
1969 GAATTC
HindIII 1391 AAGCTT A^AGCTT
2475 AAGCTT
SapI 651 GAAGAGC GCTCTTC(1/4)
2643 GCTCTTC
SbfI 2777 CCTGCAGG CCTGCA^GG
3076 CCTGCAGG
Sse8387I 2777 CCTGCAGG CCTGCA^GG
3076 CCTGCAGG
SspI 3475 AATATT AAT^ATT
5881 AATATT
XbaI 3773 TCTAGA T^CTAGA
5456 TCTAGA
XcmI 2755 CCAGTCGTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4659 CCAAAGACCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoO109I EcoRII EcoRV
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI XmnI