################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## Acc65I AccIII AclI AgeI AscI AseI AsnI Asp718I AspI AviII BlpI Bpu1102I BsaWI BseAI BsiWI BspDI BspEI BstEII BstPI CelII ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I FseI FspI KpnI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SexAI SfiI SnaBI SpeI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflII 6258 CTTAAG C^TTAAG AhdI 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5464 GTGCAC G^TGCAC ApaLI 5464 GTGCAC G^TGCAC AspEI 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 4591 GGCGCC GGCGC^C BbrPI 3356 CACGTG CAC^GTG BcgI 4440 GCAGGGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3017 GTATCC GTATCC(6/5) BclI 2093 TGATCA T^GATCA BfrI 6258 CTTAAG C^TTAAG BsaBI 1366 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2107 GAGACC GGTCTC(1/5) BsiEI 4943 CGACCG CG[GA][CT]^CG Bsp1407I 1709 TGTACA T^GTACA BspHI 1641 TCATGA T^CATGA BspLU11I 5898 ACATGT A^CATGT BsrGI 1709 TGTACA T^GTACA BssHII 4506 GCGCGC G^CGCGC BssSI 4034 CACGAG CACGAG(-5/-1) BstBI 3960 TTCGAA TT^CGAA DraI 5386 TTTAAA TTT^AAA DraIII 5065 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1369 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2724 CCTACTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 2093 TGATCA T^GATCA HpaI 5699 GTTAAC GTT^AAC KasI 4591 GGCGCC G^GCGCC KspI 4727 CCGCGG CCGC^GG MamI 1366 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 4591 GGCGCC GG^CGCC NcoI 4668 CCATGG C^CATGG NspV 3960 TTCGAA TT^CGAA PaeR7I 3797 CTCGAG C^TCGAG PmaCI 3356 CACGTG CAC^GTG PmlI 3356 CACGTG CAC^GTG PshAI 4971 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1641 TCATGA T^CATGA SacI 4933 GAGCTC GAGCT^C SacII 4727 CCGCGG CCGC^GG SalI 4941 GTCGAC G^TCGAC ScaI 4574 AGTACT AGT^ACT SfoI 4591 GGCGCC GGC^GCC SfuI 3960 TTCGAA TT^CGAA SgrAI 94 CACCGGCG C[GA]^CCGG[CT]G SspBI 1709 TGTACA T^GTACA XhoI 3797 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AatII 3375 GACGTC GACGT^C 3733 GACGTC AflIII 3355 ACACGT A^C[GA][CT]GT 5898 ACATGT BamHI 4291 GGATCC G^GATCC 4745 GGATCC BsaAI 1586 TACGTG [CT]AC^GT[GA] 3356 CACGTG BsmBI 2662 CGTCTC CGTCTC(1/5) 4969 GAGACG BsrFI 95 ACCGGC [GA]^CCGG[CT] 1308 ACCGGC Bst1107I 2888 GTATAC GTA^TAC 4120 GTATAC BstZ17I 2888 GTATAC GTA^TAC 4120 GTATAC Cfr10I 95 ACCGGC [GA]^CCGG[CT] 1308 ACCGGC EcoRI 1075 GAATTC G^AATTC 1969 GAATTC HindIII 1391 AAGCTT A^AGCTT 2475 AAGCTT SapI 651 GAAGAGC GCTCTTC(1/4) 2643 GCTCTTC SbfI 2777 CCTGCAGG CCTGCA^GG 3076 CCTGCAGG Sse8387I 2777 CCTGCAGG CCTGCA^GG 3076 CCTGCAGG SspI 3475 AATATT AAT^ATT 5881 AATATT XbaI 3773 TCTAGA T^CTAGA 5456 TCTAGA XcmI 2755 CCAGTCGTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4659 CCAAAGACCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI Asp700I AspHI AvaI AvaII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BlnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoO109I EcoRII EcoRV EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI