################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccIII AclI AgeI AscI AspI AviII AvrII BbrPI BlnI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BstBI Cfr10I ClaI CpoI DraIII EcoRV EcoT22I FseI FspI MluI MroI NaeI NdeI NgoMIV NheI NotI NsiI NspV PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SbfI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 2034 ACATGT A^C[GA][CT]GT AhdI 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2007 AGCGCT AGC^GCT AseI 4460 ATTAAT AT^TAAT AsnI 4460 ATTAAT AT^TAAT AspEI 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 890 GGCGCC GGCGC^C BcgI 2859 CGATGAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1887 TGATCA T^GATCA BsaAI 4194 TACGTG [CT]AC^GT[GA] BsaBI 6115 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 2 CGGCCG CG[GA][CT]^CG BsmBI 6034 GAGACG CGTCTC(1/5) BspLU11I 2034 ACATGT A^CATGT BssHII 75 GCGCGC G^CGCGC BstXI 1527 CCAGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 2 CGGCCG C^GGCCG Eam1105I 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2 CGGCCG C^GGCCG Eco47III 2007 AGCGCT AGC^GCT Eco52I 2 CGGCCG C^GGCCG EcoNI 2561 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1887 TGATCA T^GATCA HpaI 873 GTTAAC GTT^AAC KasI 890 GGCGCC G^GCGCC MamI 6115 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4480 CAATTG C^AATTG MunI 4480 CAATTG C^AATTG NarI 890 GGCGCC GG^CGCC NruI 102 TCGCGA TCG^CGA PacI 3573 TTAATTAA TTAAT^TAA PaeR7I 2799 CTCGAG C^TCGAG PshBI 4460 ATTAAT AT^TAAT SacI 5758 GAGCTC GAGCT^C SapI 5760 GCTCTTC GCTCTTC(1/4) SexAI 4402 ACCTGGT A^CC[AT]GGT SfiI 4777 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 890 GGCGCC GGC^GCC SspI 1303 AATATT AAT^ATT XhoI 2799 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 757 GACGTC GACGT^C 6036 GACGTC Asp700I 17 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1122 GAAGGGTTTC AvaI 467 CTCGGG C^[CT]CG[GA]G 2799 CTCGAG BbvCI 1413 CCTCAGC CCTCAGC(-5/-2) 5009 GCTGAGG BciVI 4562 GGATAC GTATCC(6/5) 1270 GTATCC BglII 2238 AGATCT A^GATCT 4959 AGATCT BsaI 2300 GAGACC GGTCTC(1/5) 2438 GAGACC BsmI 2940 GAATGC GAATGC(1/-1) 5258 GCATTC BsoBI 467 CTCGGG C^[CT]CG[GA]G 2799 CTCGAG BssSI 1749 CTCGTG CACGAG(-5/-1) 2259 CTCGTG Bst1107I 1224 GTATAC GTA^TAC 2320 GTATAC BstZ17I 1224 GTATAC GTA^TAC 2320 GTATAC DraI 3454 TTTAAA TTT^AAA 3647 TTTAAA DrdI 1390 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4054 GACAAGACTGTC EcoRI 606 GAATTC G^AATTC 1462 GAATTC HindIII 20 AAGCTT A^AGCTT 3070 AAGCTT KspI 211 CCGCGG CCGC^GG 267 CCGCGG PflMI 230 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5933 CCATATTTTGG PvuII 530 CAGCTG CAG^CTG 1366 CAGCTG SacII 211 CCGCGG CCGC^GG 267 CCGCGG SalI 727 GTCGAC G^TCGAC 794 GTCGAC Van91I 230 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5933 CCATATTTTGG XmnI 17 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1122 GAAGGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AcyI AfaI AflII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp718I AspHI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII