##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AgeI AscI AspI
AviII AvrII BbrPI BlnI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrFI BsrGI BstBI Cfr10I ClaI
CpoI DraIII EcoRV EcoT22I FseI
FspI MluI MroI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SbfI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 2034 ACATGT A^C[GA][CT]GT
AhdI 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2007 AGCGCT AGC^GCT
AseI 4460 ATTAAT AT^TAAT
AsnI 4460 ATTAAT AT^TAAT
AspEI 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 890 GGCGCC GGCGC^C
BcgI 2859 CGATGAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1887 TGATCA T^GATCA
BsaAI 4194 TACGTG [CT]AC^GT[GA]
BsaBI 6115 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 6034 GAGACG CGTCTC(1/5)
BspLU11I 2034 ACATGT A^CATGT
BssHII 75 GCGCGC G^CGCGC
BstXI 1527 CCAGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 2 CGGCCG C^GGCCG
Eam1105I 5852 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco47III 2007 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
EcoNI 2561 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1887 TGATCA T^GATCA
HpaI 873 GTTAAC GTT^AAC
KasI 890 GGCGCC G^GCGCC
MamI 6115 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4480 CAATTG C^AATTG
MunI 4480 CAATTG C^AATTG
NarI 890 GGCGCC GG^CGCC
NruI 102 TCGCGA TCG^CGA
PacI 3573 TTAATTAA TTAAT^TAA
PaeR7I 2799 CTCGAG C^TCGAG
PshBI 4460 ATTAAT AT^TAAT
SacI 5758 GAGCTC GAGCT^C
SapI 5760 GCTCTTC GCTCTTC(1/4)
SexAI 4402 ACCTGGT A^CC[AT]GGT
SfiI 4777 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 890 GGCGCC GGC^GCC
SspI 1303 AATATT AAT^ATT
XhoI 2799 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 757 GACGTC GACGT^C
6036 GACGTC
Asp700I 17 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1122 GAAGGGTTTC
AvaI 467 CTCGGG C^[CT]CG[GA]G
2799 CTCGAG
BbvCI 1413 CCTCAGC CCTCAGC(-5/-2)
5009 GCTGAGG
BciVI 4562 GGATAC GTATCC(6/5)
1270 GTATCC
BglII 2238 AGATCT A^GATCT
4959 AGATCT
BsaI 2300 GAGACC GGTCTC(1/5)
2438 GAGACC
BsmI 2940 GAATGC GAATGC(1/-1)
5258 GCATTC
BsoBI 467 CTCGGG C^[CT]CG[GA]G
2799 CTCGAG
BssSI 1749 CTCGTG CACGAG(-5/-1)
2259 CTCGTG
Bst1107I 1224 GTATAC GTA^TAC
2320 GTATAC
BstZ17I 1224 GTATAC GTA^TAC
2320 GTATAC
DraI 3454 TTTAAA TTT^AAA
3647 TTTAAA
DrdI 1390 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4054 GACAAGACTGTC
EcoRI 606 GAATTC G^AATTC
1462 GAATTC
HindIII 20 AAGCTT A^AGCTT
3070 AAGCTT
KspI 211 CCGCGG CCGC^GG
267 CCGCGG
PflMI 230 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5933 CCATATTTTGG
PvuII 530 CAGCTG CAG^CTG
1366 CAGCTG
SacII 211 CCGCGG CCGC^GG
267 CCGCGG
SalI 727 GTCGAC G^TCGAC
794 GTCGAC
Van91I 230 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5933 CCATATTTTGG
XmnI 17 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1122 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI Asp718I AspHI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII