################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI BamHI BbeI BbrPI BbsI BfrI BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspMI BstEII BstPI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FseI Hin1I HpaI KasI KpnI MaeII MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 5358 GTATAC GT^[AC][GT]AC AflIII 3581 ACATGT A^C[GA][CT]GT AocI 328 CCTTAGG CC^T[ACGT]AGG AseI 1379 ATTAAT AT^TAAT AsnI 1379 ATTAAT AT^TAAT AviII 306 TGCGCA TGC^GCA AvrII 4860 CCTAGG C^CTAGG BcgI 4016 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2579 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4860 CCTAGG C^CTAGG BsaBI 1774 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 4365 TGTACA T^GTACA BspLU11I 3581 ACATGT A^CATGT BsrDI 966 CATTGC GCAATG(2/0) BsrFI 5498 GCCGGT [GA]^CCGG[CT] BsrGI 4365 TGTACA T^GTACA Bst1107I 5358 GTATAC GTA^TAC BstAPI 3683 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 934 TTCGAA TT^CGAA BstZ17I 5358 GTATAC GTA^TAC Bsu36I 328 CCTTAGG CC^T[ACGT]AGG Cfr10I 5498 GCCGGT [GA]^CCGG[CT] DraIII 776 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 328 CCTTAGG CC^T[ACGT]AGG FspI 306 TGCGCA TGC^GCA HaeII 4615 AGCGCC [GA]GCGC^[CT] HindIII 3508 AAGCTT A^AGCTT KspI 48 CCGCGG CCGC^GG MamI 1774 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2427 CAATTG C^AATTG MunI 2427 CAATTG C^AATTG NcoI 1943 CCATGG C^CATGG NspV 934 TTCGAA TT^CGAA PshBI 1379 ATTAAT AT^TAAT PvuII 848 CAGCTG CAG^CTG SacI 5513 GAGCTC GAGCT^C SacII 48 CCGCGG CCGC^GG SapI 4852 GAAGAGC GCTCTTC(1/4) SfuI 934 TTCGAA TT^CGAA SphI 1196 GCATGC GCATG^C SspBI 4365 TGTACA T^GTACA StuI 811 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BciVI 3389 GGATAC GTATCC(6/5) 4898 GGATAC BclI 962 TGATCA T^GATCA 5668 TGATCA BglII 1277 AGATCT A^GATCT 1574 AGATCT BlpI 245 GCTTAGC GC^T[ACGT]AGC 3568 GCTCAGC Bpu1102I 245 GCTTAGC GC^T[ACGT]AGC 3568 GCTCAGC BsmBI 2557 GAGACG CGTCTC(1/5) 3625 GAGACG BssHII 4057 GCGCGC G^CGCGC 4059 GCGCGC BssSI 555 CTCGTG CACGAG(-5/-1) 2608 CTCGTG CelII 245 GCTTAGC GC^T[ACGT]AGC 3568 GCTCAGC DraII 789 AGGACCC [GA]G^G[ACGT]CC[CT] 1011 GGGTCCT EcoO109I 789 AGGACCC [GA]G^G[ACGT]CC[CT] 1011 GGGTCCT EcoRI 480 GAATTC G^AATTC 1296 GAATTC EcoRV 920 GATATC GAT^ATC 5229 GATATC EcoT22I 1706 ATGCAT ATGCA^T 4445 ATGCAT FbaI 962 TGATCA T^GATCA 5668 TGATCA HgaI 1238 GCGTC GACGC(5/10) 4200 GCGTC NsiI 1706 ATGCAT ATGCA^T 4445 ATGCAT PpuMI 789 AGGACCC [GA]G^G[AT]CC[CT] 1011 GGGTCCT XbaI 1103 TCTAGA T^CTAGA 4649 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SpeI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII