##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AvaI BamHI BbeI BbrPI
BbsI BfrI BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspMI BstEII BstPI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
FseI Hin1I HpaI KasI KpnI
MaeII MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
Van91I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 5358 GTATAC GT^[AC][GT]AC
AflIII 3581 ACATGT A^C[GA][CT]GT
AocI 328 CCTTAGG CC^T[ACGT]AGG
AseI 1379 ATTAAT AT^TAAT
AsnI 1379 ATTAAT AT^TAAT
AviII 306 TGCGCA TGC^GCA
AvrII 4860 CCTAGG C^CTAGG
BcgI 4016 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2579 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4860 CCTAGG C^CTAGG
BsaBI 1774 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4365 TGTACA T^GTACA
BspLU11I 3581 ACATGT A^CATGT
BsrDI 966 CATTGC GCAATG(2/0)
BsrFI 5498 GCCGGT [GA]^CCGG[CT]
BsrGI 4365 TGTACA T^GTACA
Bst1107I 5358 GTATAC GTA^TAC
BstAPI 3683 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 934 TTCGAA TT^CGAA
BstZ17I 5358 GTATAC GTA^TAC
Bsu36I 328 CCTTAGG CC^T[ACGT]AGG
Cfr10I 5498 GCCGGT [GA]^CCGG[CT]
DraIII 776 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 328 CCTTAGG CC^T[ACGT]AGG
FspI 306 TGCGCA TGC^GCA
HaeII 4615 AGCGCC [GA]GCGC^[CT]
HindIII 3508 AAGCTT A^AGCTT
KspI 48 CCGCGG CCGC^GG
MamI 1774 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2427 CAATTG C^AATTG
MunI 2427 CAATTG C^AATTG
NcoI 1943 CCATGG C^CATGG
NspV 934 TTCGAA TT^CGAA
PshBI 1379 ATTAAT AT^TAAT
PvuII 848 CAGCTG CAG^CTG
SacI 5513 GAGCTC GAGCT^C
SacII 48 CCGCGG CCGC^GG
SapI 4852 GAAGAGC GCTCTTC(1/4)
SfuI 934 TTCGAA TT^CGAA
SphI 1196 GCATGC GCATG^C
SspBI 4365 TGTACA T^GTACA
StuI 811 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BciVI 3389 GGATAC GTATCC(6/5)
4898 GGATAC
BclI 962 TGATCA T^GATCA
5668 TGATCA
BglII 1277 AGATCT A^GATCT
1574 AGATCT
BlpI 245 GCTTAGC GC^T[ACGT]AGC
3568 GCTCAGC
Bpu1102I 245 GCTTAGC GC^T[ACGT]AGC
3568 GCTCAGC
BsmBI 2557 GAGACG CGTCTC(1/5)
3625 GAGACG
BssHII 4057 GCGCGC G^CGCGC
4059 GCGCGC
BssSI 555 CTCGTG CACGAG(-5/-1)
2608 CTCGTG
CelII 245 GCTTAGC GC^T[ACGT]AGC
3568 GCTCAGC
DraII 789 AGGACCC [GA]G^G[ACGT]CC[CT]
1011 GGGTCCT
EcoO109I 789 AGGACCC [GA]G^G[ACGT]CC[CT]
1011 GGGTCCT
EcoRI 480 GAATTC G^AATTC
1296 GAATTC
EcoRV 920 GATATC GAT^ATC
5229 GATATC
EcoT22I 1706 ATGCAT ATGCA^T
4445 ATGCAT
FbaI 962 TGATCA T^GATCA
5668 TGATCA
HgaI 1238 GCGTC GACGC(5/10)
4200 GCGTC
NsiI 1706 ATGCAT ATGCA^T
4445 ATGCAT
PpuMI 789 AGGACCC [GA]G^G[AT]CC[CT]
1011 GGGTCCT
XbaI 1103 TCTAGA T^CTAGA
4649 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmlI SpeI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII