##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I AspEI AviII
BamHI BbrPI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HpaI KspI
MamI MluI MroI MvnI NaeI
NgoMIV NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RsrII
SacII SapI SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI Van91I
XmnI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3074 GGTACC G^GTACC
AlwNI 5542 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 3074 GGTACC G^GTACC
BcgI 4617 GCACAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4584 AGATCT A^GATCT
BsmBI 1229 GAGACG CGTCTC(1/5)
Bsp1407I 2686 TGTACA T^GTACA
BspHI 765 TCATGA T^CATGA
BsrGI 2686 TGTACA T^GTACA
DsaI 5480 CCATGG C^C[GA][CT]GG
EcoT22I 2240 ATGCAT ATGCA^T
HgaI 4440 GACGC GACGC(5/10)
KpnI 3074 GGTACC GGTAC^C
MfeI 5217 CAATTG C^AATTG
MunI 5217 CAATTG C^AATTG
NcoI 5480 CCATGG C^CATGG
NdeI 2379 CATATG CA^TATG
NsiI 2240 ATGCAT ATGCA^T
PaeR7I 240 CTCGAG C^TCGAG
RcaI 765 TCATGA T^CATGA
SalI 76 GTCGAC G^TCGAC
SexAI 372 ACCTGGT A^CC[AT]GGT
SfcI 4031 CTGTAG C^T[GA][CT]AG
SpeI 913 ACTAGT A^CTAGT
SspBI 2686 TGTACA T^GTACA
XbaI 1032 TCTAGA T^CTAGA
XhoI 240 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspI 3322 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4702 GACTCTGTC
AvrII 1708 CCTAGG C^CTAGG
4635 CCTAGG
BalI 3964 TGGCCA TGG^CCA
4130 TGGCCA
BbvCI 4054 GCTGAGG CCTCAGC(-5/-2)
4611 GCTGAGG
BlnI 1708 CCTAGG C^CTAGG
4635 CCTAGG
DraII 212 GGGTCCT [GA]G^G[ACGT]CC[CT]
2635 AGGACCT
EarI 4943 CTCTTC CTCTTC(1/4)
4297 GAAGAG
EcoO109I 212 GGGTCCT [GA]G^G[ACGT]CC[CT]
2635 AGGACCT
HindIII 1171 AAGCTT A^AGCTT
3647 AAGCTT
Ksp632I 4943 CTCTTC CTCTTC(1/4)
4297 GAAGAG
MluNI 3964 TGGCCA TGG^CCA
4130 TGGCCA
MscI 3964 TGGCCA TGG^CCA
4130 TGGCCA
NheI 682 GCTAGC G^CTAGC
1594 GCTAGC
PflFI 3322 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4702 GACTCTGTC
PpuMI 212 GGGTCCT [GA]G^G[AT]CC[CT]
2635 AGGACCT
SacI 3524 GAGCTC GAGCT^C
4493 GAGCTC
SmaI 3424 CCCGGG CCC^GGG
4078 CCCGGG
SphI 2242 GCATGC GCATG^C
4443 GCATGC
Tth111I 3322 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4702 GACTCTGTC
XmaCI 3424 CCCGGG C^CCGGG
4078 CCCGGG
XmaI 3424 CCCGGG C^CCGGG
4078 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AocI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BciVI BclI BcnI BfaI BfrI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
Eco81I EcoNI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfoI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII