##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
BcgI BsaBI BseAI BsiWI BspDI
BspEI BspHI BssSI Bst1107I BstZ17I
ClaI DrdI EcoT22I FseI HindIII
MamI MfeI MluI MroI MunI
NheI NruI NsiI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
SalI SapI SbfI ScaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 4901 CTTAAG C^TTAAG
AhdI 4445 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 5391 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4445 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1038 TGCGCA TGC^GCA
BbeI 396 GGCGCC GGCGC^C
BbrPI 3469 CACGTG CAC^GTG
BfrI 4901 CTTAAG C^TTAAG
BglII 1927 AGATCT A^GATCT
BsmBI 590 CGTCTC CGTCTC(1/5)
BspLU11I 3107 ACATGT A^CATGT
BsrBI 3397 CCGCTC CCGCTC(-3/-3)
BssHII 867 GCGCGC G^CGCGC
BstBI 2948 TTCGAA TT^CGAA
BstEII 1240 GGTCACC G^GT[ACGT]ACC
BstPI 1240 GGTCACC G^GT[ACGT]ACC
CpoI 1064 CGGACCG CG^G[AT]CCG
DraI 5926 TTTAAA TTT^AAA
EagI 2 CGGCCG C^GGCCG
Eam1105I 4445 GACGTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoO65I 1240 GGTCACC G^GT[ACGT]ACC
EcoRI 4418 GAATTC G^AATTC
EcoRV 125 GATATC GAT^ATC
FspI 1038 TGCGCA TGC^GCA
HpaI 5378 GTTAAC GTT^AAC
KasI 396 GGCGCC G^GCGCC
KspI 3310 CCGCGG CCGC^GG
NarI 396 GGCGCC GG^CGCC
NdeI 6190 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
NspV 2948 TTCGAA TT^CGAA
PmaCI 3469 CACGTG CAC^GTG
PmlI 3469 CACGTG CAC^GTG
RsrII 1064 CGGACCG CG^G[AT]CCG
SacII 3310 CCGCGG CCGC^GG
SexAI 495 ACCTGGT A^CC[AT]GGT
SfiI 574 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 396 GGCGCC GGC^GCC
SfuI 2948 TTCGAA TT^CGAA
SgrAI 1007 CGCCGGCG C[GA]^CCGG[CT]G
XbaI 4983 TCTAGA T^CTAGA
XmnI 5391 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 3107 ACATGT A^C[GA][CT]GT
3468 ACACGT
Aor51HI 1089 AGCGCT AGC^GCT
4719 AGCGCT
AseI 3446 ATTAAT AT^TAAT
6286 ATTAAT
AsnI 3446 ATTAAT AT^TAAT
6286 ATTAAT
BamHI 2582 GGATCC G^GATCC
5189 GGATCC
BclI 3145 TGATCA T^GATCA
4656 TGATCA
BsiEI 2 CGGCCG CG[GA][CT]^CG
730 CGACCG
Bsp1407I 2397 TGTACA T^GTACA
4149 TGTACA
BsrGI 2397 TGTACA T^GTACA
4149 TGTACA
BstAPI 85 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1421 GCAGGCACTGC
Eco47III 1089 AGCGCT AGC^GCT
4719 AGCGCT
FbaI 3145 TGATCA T^GATCA
4656 TGATCA
HincII 2189 GTCAAC GT[CT]^[GA]AC
5378 GTTAAC
HindII 2189 GTCAAC GT[CT]^[GA]AC
5378 GTTAAC
PshAI 369 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3412 GACCAGCGTC
PshBI 3446 ATTAAT AT^TAAT
6286 ATTAAT
PvuII 917 CAGCTG CAG^CTG
4260 CAGCTG
SacI 1113 GAGCTC GAGCT^C
1720 GAGCTC
SspBI 2397 TGTACA T^GTACA
4149 TGTACA
StuI 582 AGGCCT AGG^CCT
4327 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp718I AspHI AspI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI