##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
AscI AseI AsnI AspEI AviII
BbeI BclI BsaBI BspDI BspHI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoO65I EcoRV FbaI
FseI FspI HpaI KasI KspI
MamI MfeI MluI MunI NarI
NotI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SfiI SfoI SfuI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4278 CTTAAG C^TTAAG
Aor51HI 3268 AGCGCT AGC^GCT
Asp700I 4800 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5957 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4235 CCTAGG C^CTAGG
BamHI 3308 GGATCC G^GATCC
BbrPI 4016 CACGTG CAC^GTG
BciVI 3373 GTATCC GTATCC(6/5)
BfrI 4278 CTTAAG C^TTAAG
BglI 3914 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4235 CCTAGG C^CTAGG
Bsp1407I 3778 TGTACA T^GTACA
BsrDI 2757 GCAATG GCAATG(2/0)
BsrGI 3778 TGTACA T^GTACA
BssHII 117 GCGCGC G^CGCGC
DrdI 3018 GACAATAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3268 AGCGCT AGC^GCT
EcoRI 4642 GAATTC G^AATTC
EcoT22I 4939 ATGCAT ATGCA^T
HindIII 3127 AAGCTT A^AGCTT
NdeI 1289 CATATG CA^TATG
NheI 305 GCTAGC G^CTAGC
NruI 2483 TCGCGA TCG^CGA
NsiI 4939 ATGCAT ATGCA^T
PflFI 5957 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4016 CACGTG CAC^GTG
PmlI 4016 CACGTG CAC^GTG
PshAI 29 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1016 GTCGAC G^TCGAC
SbfI 959 CCTGCAGG CCTGCA^GG
SexAI 607 ACCAGGT A^CC[AT]GGT
SmaI 3058 CCCGGG CCC^GGG
SpeI 1479 ACTAGT A^CTAGT
SphI 2830 GCATGC GCATG^C
Sse8387I 959 CCTGCAGG CCTGCA^GG
SspBI 3778 TGTACA T^GTACA
SspI 5155 AATATT AAT^ATT
Tth111I 5957 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4605 TCTAGA T^CTAGA
XmaCI 3058 CCCGGG C^CCGGG
XmaI 3058 CCCGGG C^CCGGG
XmnI 4800 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 966 GGTACC G^GTACC
3356 GGTACC
AccIII 5001 TCCGGA T^CCGGA
5079 TCCGGA
AcyI 330 GACGCC G[GA]^CG[CT]C
1564 GACGCC
Alw44I 2330 GTGCAC G^TGCAC
5223 GTGCAC
ApaLI 2330 GTGCAC G^TGCAC
5223 GTGCAC
Asp718I 966 GGTACC G^GTACC
3356 GGTACC
BbsI 711 GAAGAC GAAGAC(2/6)
5206 GAAGAC
BglII 1696 AGATCT A^GATCT
4272 AGATCT
BpuAI 711 GAAGAC GAAGAC(2/6)
5206 GAAGAC
BsaAI 3279 CACGTA [CT]AC^GT[GA]
4016 CACGTG
BsaHI 330 GACGCC G[GA]^CG[CT]C
1564 GACGCC
BsaI 1954 GGTCTC GGTCTC(1/5)
4901 GGTCTC
BseAI 5001 TCCGGA T^CCGGA
5079 TCCGGA
BsiEI 279 CGGCCG CG[GA][CT]^CG
422 CGGCCG
BsiWI 2806 CGTACG C^GTACG
3281 CGTACG
BsmBI 5323 CGTCTC CGTCTC(1/5)
3255 GAGACG
BsmI 3761 GAATGC GAATGC(1/-1)
5866 GAATGC
BspEI 5001 TCCGGA T^CCGGA
5079 TCCGGA
BsrBI 195 GAGCGG CCGCTC(-3/-3)
3535 GAGCGG
BsrFI 3159 GCCGGC [GA]^CCGG[CT]
4502 GCCGGC
Cfr10I 3159 GCCGGC [GA]^CCGG[CT]
4502 GCCGGC
EagI 279 CGGCCG C^GGCCG
422 CGGCCG
EclXI 279 CGGCCG C^GGCCG
422 CGGCCG
Eco52I 279 CGGCCG C^GGCCG
422 CGGCCG
HgaI 330 GACGC GACGC(5/10)
1564 GACGC
Hin1I 330 GACGCC G[GA]^CG[CT]C
1564 GACGCC
HincII 1016 GTCGAC GT[CT]^[GA]AC
3617 GTTGAC
HindII 1016 GTCGAC GT[CT]^[GA]AC
3617 GTTGAC
KpnI 966 GGTACC GGTAC^C
3356 GGTACC
MroI 5001 TCCGGA T^CCGGA
5079 TCCGGA
NaeI 3159 GCCGGC GCC^GGC
4502 GCCGGC
NgoMIV 3159 GCCGGC G^CCGGC
4502 GCCGGC
PaeR7I 1442 CTCGAG C^TCGAG
2956 CTCGAG
ScaI 632 AGTACT AGT^ACT
1714 AGTACT
XhoI 1442 CTCGAG C^TCGAG
2956 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII