##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII BbeI BcgI BglII
BsaBI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SnaBI Sse8387I
SspBI XbaI
###################################
# ONE CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 5945 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5945 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2167 GGATAC GTATCC(6/5)
BsmBI 2838 CGTCTC CGTCTC(1/5)
BsrBI 3 CCGCTC CCGCTC(-3/-3)
DraIII 2348 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 5945 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MfeI 6013 CAATTG C^AATTG
MunI 6013 CAATTG C^AATTG
NheI 872 GCTAGC G^CTAGC
PaeR7I 435 CTCGAG C^TCGAG
PflMI 834 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 5352 ACCTGGT A^CC[AT]GGT
SgrAI 2684 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1072 CCCGGG CCC^GGG
SwaI 5225 ATTTAAAT ATTT^AAAT
Van91I 834 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 435 CTCGAG C^TCGAG
XmaCI 1072 CCCGGG C^CCGGG
XmaI 1072 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1372 CTTAAG C^TTAAG
1912 CTTAAG
AspI 688 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2362 GACAGGGTC
BfrI 1372 CTTAAG C^TTAAG
1912 CTTAAG
BlpI 732 GCTGAGC GC^T[ACGT]AGC
3933 GCTGAGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
3933 GCTGAGC
BsaI 830 GAGACC GGTCTC(1/5)
3624 GAGACC
BsrFI 573 ACCGGC [GA]^CCGG[CT]
2685 GCCGGT
BssSI 5311 CACGAG CACGAG(-5/-1)
93 CTCGTG
BstAPI 2201 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3159 GCACACTTTGC
BstBI 165 TTCGAA TT^CGAA
5287 TTCGAA
CelII 732 GCTGAGC GC^T[ACGT]AGC
3933 GCTGAGC
Cfr10I 573 ACCGGC [GA]^CCGG[CT]
2685 GCCGGT
HaeII 465 GGCGCT [GA]GCGC^[CT]
2005 AGCGCC
HindIII 316 AAGCTT A^AGCTT
3749 AAGCTT
NspV 165 TTCGAA TT^CGAA
5287 TTCGAA
PflFI 688 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2362 GACAGGGTC
PshAI 3892 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5077 GACTACAGTC
SapI 1471 GAAGAGC GCTCTTC(1/4)
2384 GAAGAGC
SfuI 165 TTCGAA TT^CGAA
5287 TTCGAA
SpeI 419 ACTAGT A^CTAGT
4865 ACTAGT
SspI 3836 AATATT AAT^ATT
5862 AATATT
StuI 771 AGGCCT AGG^CCT
1861 AGGCCT
Tth111I 688 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2362 GACAGGGTC
XcmI 1951 CCATCCTTCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5033 CCATTGAATTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII AvrII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI