##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaLI BbrPI BfrI BsaBI BsiWI
BspDI BspMI BstEII BstPI ClaI
CpoI EcoNI EcoO65I FseI KspI
MamI MfeI MluI MunI NdeI
NotI PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SnaBI
Sse8387I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 2341 TCCGGA T^CCGGA
AflIII 3990 ACATGT A^C[GA][CT]GT
Aor51HI 356 AGCGCT AGC^GCT
AscI 313 GGCGCGCC GG^CGCGCC
AseI 3420 ATTAAT AT^TAAT
AsnI 3420 ATTAAT AT^TAAT
Asp700I 3772 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1490 TGCGCA TGC^GCA
AvrII 3707 CCTAGG C^CTAGG
BclI 2679 TGATCA T^GATCA
BglI 5540 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5122 AGATCT A^GATCT
BlnI 3707 CCTAGG C^CTAGG
BseAI 2341 TCCGGA T^CCGGA
Bsp1407I 1209 TGTACA T^GTACA
BspEI 2341 TCCGGA T^CCGGA
BspLU11I 3990 ACATGT A^CATGT
BsrGI 1209 TGTACA T^GTACA
BssSI 6328 CACGAG CACGAG(-5/-1)
Bst1107I 5107 GTATAC GTA^TAC
BstBI 700 TTCGAA TT^CGAA
BstZ17I 5107 GTATAC GTA^TAC
DrdI 404 GACGCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 356 AGCGCT AGC^GCT
EcoRI 4104 GAATTC G^AATTC
FbaI 2679 TGATCA T^GATCA
FspI 1490 TGCGCA TGC^GCA
HindIII 1393 AAGCTT A^AGCTT
HpaI 1025 GTTAAC GTT^AAC
MroI 2341 TCCGGA T^CCGGA
NruI 111 TCGCGA TCG^CGA
NspV 700 TTCGAA TT^CGAA
PflMI 1535 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 261 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 3420 ATTAAT AT^TAAT
PstI 2129 CTGCAG CTGCA^G
SexAI 847 ACCTGGT A^CC[AT]GGT
SfuI 700 TTCGAA TT^CGAA
SgrAI 187 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3027 GCATGC GCATG^C
SspBI 1209 TGTACA T^GTACA
SspI 3493 AATATT AAT^ATT
Van91I 1535 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4017 TCTAGA T^CTAGA
XmnI 3772 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 368 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
4650 CAGTTGCTG
ApaI 300 GGGCCC GGGCC^C
2518 GGGCCC
AspI 846 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6537 GACTCCGTC
BalI 994 TGGCCA TGG^CCA
3640 TGGCCA
BamHI 2936 GGATCC G^GATCC
6118 GGATCC
BbvCI 1952 GCTGAGG CCTCAGC(-5/-2)
6446 GCTGAGG
BcgI 2087 GCACCTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2407 GCACGGTCTTCG
BlpI 2534 GCTGAGC GC^T[ACGT]AGC
2887 GCTAAGC
Bpu1102I 2534 GCTGAGC GC^T[ACGT]AGC
2887 GCTAAGC
BsaAI 1161 TACGTG [CT]AC^GT[GA]
2496 TACGTG
BsaWI 1411 ACCGGA [AT]^CCGG[AT]
2341 TCCGGA
BseRI 2732 GAGGAG GAGGAG(10/8)
6076 GAGGAG
BsrBI 61 CCGCTC CCGCTC(-3/-3)
118 CCGCTC
BstAPI 1604 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1878 GCAAATGTTGC
CelII 2534 GCTGAGC GC^T[ACGT]AGC
2887 GCTAAGC
DraIII 2876 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
5285 CACTCTGTG
EcoRV 1659 GATATC GAT^ATC
4625 GATATC
EcoT22I 1529 ATGCAT ATGCA^T
2696 ATGCAT
MluNI 994 TGGCCA TGG^CCA
3640 TGGCCA
MscI 994 TGGCCA TGG^CCA
3640 TGGCCA
NheI 15 GCTAGC G^CTAGC
19 GCTAGC
NsiI 1529 ATGCAT ATGCA^T
2696 ATGCAT
PaeR7I 360 CTCGAG C^TCGAG
5091 CTCGAG
PflFI 846 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6537 GACTCCGTC
SpeI 1692 ACTAGT A^CTAGT
5111 ACTAGT
StuI 1956 AGGCCT AGG^CCT
5162 AGGCCT
SwaI 542 ATTTAAAT ATTT^AAAT
4555 ATTTAAAT
Tth111I 846 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6537 GACTCCGTC
XcmI 4054 CCAGGAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5854 CCAGCTTCCCTGTGG
XhoI 360 CTCGAG C^TCGAG
5091 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AhdI AluI AlwI
AocI ApoI Asp718I AspEI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI