##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
AscI AseI AsnI AspEI AviII
BbeI BclI BsaBI BspDI BspHI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII Eam1105I EcoO65I EcoRV FbaI
FseI FspI HpaI KasI KspI
MamI MfeI MluI MunI NarI
NotI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SfiI SfoI SfuI SgrAI
SnaBI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 4273 CTTAAG C^TTAAG
Aor51HI 3263 AGCGCT AGC^GCT
Asp700I 4795 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5952 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4230 CCTAGG C^CTAGG
BamHI 3303 GGATCC G^GATCC
BbrPI 4011 CACGTG CAC^GTG
BciVI 3368 GTATCC GTATCC(6/5)
BfrI 4273 CTTAAG C^TTAAG
BglI 3909 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4230 CCTAGG C^CTAGG
Bsp1407I 3773 TGTACA T^GTACA
BsrDI 2752 GCAATG GCAATG(2/0)
BsrGI 3773 TGTACA T^GTACA
BssHII 112 GCGCGC G^CGCGC
DrdI 3013 GACAATAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3263 AGCGCT AGC^GCT
EcoRI 4637 GAATTC G^AATTC
EcoT22I 4934 ATGCAT ATGCA^T
HindIII 3122 AAGCTT A^AGCTT
NdeI 1284 CATATG CA^TATG
NheI 300 GCTAGC G^CTAGC
NruI 2478 TCGCGA TCG^CGA
NsiI 4934 ATGCAT ATGCA^T
PflFI 5952 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4011 CACGTG CAC^GTG
PmlI 4011 CACGTG CAC^GTG
PshAI 24 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1011 GTCGAC G^TCGAC
SbfI 954 CCTGCAGG CCTGCA^GG
SexAI 602 ACCAGGT A^CC[AT]GGT
SmaI 3053 CCCGGG CCC^GGG
SpeI 1474 ACTAGT A^CTAGT
SphI 2825 GCATGC GCATG^C
Sse8387I 954 CCTGCAGG CCTGCA^GG
SspBI 3773 TGTACA T^GTACA
SspI 5150 AATATT AAT^ATT
Tth111I 5952 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4600 TCTAGA T^CTAGA
XmaCI 3053 CCCGGG C^CCGGG
XmaI 3053 CCCGGG C^CCGGG
XmnI 4795 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 961 GGTACC G^GTACC
3351 GGTACC
AccIII 4996 TCCGGA T^CCGGA
5074 TCCGGA
AcyI 325 GACGCC G[GA]^CG[CT]C
1559 GACGCC
Alw44I 2325 GTGCAC G^TGCAC
5218 GTGCAC
ApaLI 2325 GTGCAC G^TGCAC
5218 GTGCAC
Asp718I 961 GGTACC G^GTACC
3351 GGTACC
BbsI 706 GAAGAC GAAGAC(2/6)
5201 GAAGAC
BglII 1691 AGATCT A^GATCT
4267 AGATCT
BpuAI 706 GAAGAC GAAGAC(2/6)
5201 GAAGAC
BsaAI 3274 CACGTA [CT]AC^GT[GA]
4011 CACGTG
BsaHI 325 GACGCC G[GA]^CG[CT]C
1559 GACGCC
BsaI 1949 GGTCTC GGTCTC(1/5)
4896 GGTCTC
BseAI 4996 TCCGGA T^CCGGA
5074 TCCGGA
BsiEI 274 CGGCCG CG[GA][CT]^CG
417 CGGCCG
BsiWI 2801 CGTACG C^GTACG
3276 CGTACG
BsmBI 5318 CGTCTC CGTCTC(1/5)
3250 GAGACG
BsmI 3756 GAATGC GAATGC(1/-1)
5861 GAATGC
BspEI 4996 TCCGGA T^CCGGA
5074 TCCGGA
BsrBI 190 GAGCGG CCGCTC(-3/-3)
3530 GAGCGG
BsrFI 3154 GCCGGC [GA]^CCGG[CT]
4497 GCCGGC
Cfr10I 3154 GCCGGC [GA]^CCGG[CT]
4497 GCCGGC
EagI 274 CGGCCG C^GGCCG
417 CGGCCG
EclXI 274 CGGCCG C^GGCCG
417 CGGCCG
Eco52I 274 CGGCCG C^GGCCG
417 CGGCCG
HgaI 325 GACGC GACGC(5/10)
1559 GACGC
Hin1I 325 GACGCC G[GA]^CG[CT]C
1559 GACGCC
HincII 1011 GTCGAC GT[CT]^[GA]AC
3612 GTTGAC
HindII 1011 GTCGAC GT[CT]^[GA]AC
3612 GTTGAC
KpnI 961 GGTACC GGTAC^C
3351 GGTACC
MroI 4996 TCCGGA T^CCGGA
5074 TCCGGA
NaeI 3154 GCCGGC GCC^GGC
4497 GCCGGC
NgoMIV 3154 GCCGGC G^CCGGC
4497 GCCGGC
PaeR7I 1437 CTCGAG C^TCGAG
2951 CTCGAG
ScaI 627 AGTACT AGT^ACT
1709 AGTACT
XhoI 1437 CTCGAG C^TCGAG
2951 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII