##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BciVI BclI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NheI NotI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tsp509I XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2516 GTCTAC GT^[AC][GT]AC
AflIII 477 ACGTGT A^C[GA][CT]GT
Alw44I 653 GTGCAC G^TGCAC
ApaLI 653 GTGCAC G^TGCAC
Asp700I 406 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1440 TGCGCA TGC^GCA
BamHI 433 GGATCC G^GATCC
BfaI 1946 CTAG C^TAG
BlpI 2231 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2231 GCTCAGC GC^T[ACGT]AGC
BsiEI 708 CGGTCG CG[GA][CT]^CG
BspDI 2639 ATCGAT AT^CGAT
CelII 2231 GCTCAGC GC^T[ACGT]AGC
ClaI 2639 ATCGAT AT^CGAT
FspI 1440 TGCGCA TGC^GCA
HindIII 2579 AAGCTT A^AGCTT
MaeI 1946 CTAG C^TAG
NdeI 1449 CATATG CA^TATG
NruI 1168 TCGCGA TCG^CGA
NspI 3240 GCATGT [GA]CATG^[CT]
PaeR7I 3131 CTCGAG C^TCGAG
SfiI 2212 GGCCCCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XhoI 3131 CTCGAG C^TCGAG
XmnI 406 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AatII 1454 GACGTC GACGT^C
2033 GACGTC
AspI 299 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1646 GACGATGTC
DraIII 647 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
656 CACAGCGTG
NcoI 117 CCATGG C^CATGG
3660 CCATGG
PflFI 299 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1646 GACGATGTC
PflMI 190 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3660 CCATGGGGTGG
SapI 1358 GAAGAGC GCTCTTC(1/4)
3019 GAAGAGC
SexAI 828 ACCTGGT A^CC[AT]GGT
3278 ACCAGGT
SmaI 50 CCCGGG CCC^GGG
241 CCCGGG
Tth111I 299 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1646 GACGATGTC
Van91I 190 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3660 CCATGGGGTGG
XmaCI 50 CCCGGG C^CCGGG
241 CCCGGG
XmaI 50 CCCGGG C^CCGGG
241 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BcnI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I XcmI XhoII