################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AflII AflIII AgeI Aor51HI AscI AspI AviII BbvCI BcgI BciVI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoT22I FseI FspI HindIII HpaI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SalI SfiI SfuI SnaBI SpeI SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 4505 GGTACC G^GTACC AccIII 6799 TCCGGA T^CCGGA AcsI 1026 GAATTC [GA]^AATT[CT] ApoI 1026 GAATTC [GA]^AATT[CT] AseI 1011 ATTAAT AT^TAAT AsnI 1011 ATTAAT AT^TAAT Asp718I 4505 GGTACC G^GTACC BamHI 5943 GGATCC G^GATCC BbeI 354 GGCGCC GGCGC^C BbrPI 4893 CACGTG CAC^GTG BclI 805 TGATCA T^GATCA BsaAI 4893 CACGTG [CT]AC^GT[GA] BseAI 6799 TCCGGA T^CCGGA BsmBI 5857 GAGACG CGTCTC(1/5) BsmI 5438 GAATGC GAATGC(1/-1) BspEI 6799 TCCGGA T^CCGGA BsrDI 2257 GCAATG GCAATG(2/0) BssSI 1779 CACGAG CACGAG(-5/-1) BstEII 5545 GGTGACC G^GT[ACGT]ACC BstPI 5545 GGTGACC G^GT[ACGT]ACC EcoO65I 5545 GGTGACC G^GT[ACGT]ACC EcoRI 1026 GAATTC G^AATTC EcoRV 4996 GATATC GAT^ATC FbaI 805 TGATCA T^GATCA HincII 2830 GTCAAC GT[CT]^[GA]AC HindII 2830 GTCAAC GT[CT]^[GA]AC KasI 354 GGCGCC G^GCGCC KpnI 4505 GGTACC GGTAC^C MaeII 4894 ACGT A^CGT MroI 6799 TCCGGA T^CCGGA NarI 354 GGCGCC GG^CGCC NheI 4281 GCTAGC G^CTAGC NspI 2769 GCATGC [GA]CATG^[CT] PmaCI 4893 CACGTG CAC^GTG PmlI 4893 CACGTG CAC^GTG PshAI 2549 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1011 ATTAAT AT^TAAT SbfI 6024 CCTGCAGG CCTGCA^GG ScaI 6230 AGTACT AGT^ACT SexAI 3204 ACCAGGT A^CC[AT]GGT SfoI 354 GGCGCC GGC^GCC SgrAI 332 CGCCGGCG C[GA]^CCGG[CT]G SphI 2769 GCATGC GCATG^C Sse8387I 6024 CCTGCAGG CCTGCA^GG SspI 1014 AATATT AAT^ATT Tsp509I 1027 AATT ^AATT ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccI 6100 GTCTAC GT^[AC][GT]AC 6394 GTCTAC AvrII 1145 CCTAGG C^CTAGG 4631 CCTAGG BlnI 1145 CCTAGG C^CTAGG 4631 CCTAGG BlpI 3179 GCTGAGC GC^T[ACGT]AGC 4453 GCTAAGC Bpu1102I 3179 GCTGAGC GC^T[ACGT]AGC 4453 GCTAAGC BsaWI 5434 ACCGGA [AT]^CCGG[AT] 6799 TCCGGA BstAPI 1994 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4034 GCAGGGGTTGC CelII 3179 GCTGAGC GC^T[ACGT]AGC 4453 GCTAAGC DraIII 5029 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5136 CACCCTGTG SacI 882 GAGCTC GAGCT^C 3065 GAGCTC SapI 6858 GAAGAGC GCTCTTC(1/4) 4413 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI