##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
Aor51HI AscI AspI AviII BbvCI
BcgI BciVI BfrI BglII BsaBI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoT22I FseI
FspI HindIII HpaI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SalI SfiI SfuI SnaBI SpeI
SspBI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4505 GGTACC G^GTACC
AccIII 6799 TCCGGA T^CCGGA
AcsI 1026 GAATTC [GA]^AATT[CT]
ApoI 1026 GAATTC [GA]^AATT[CT]
AseI 1011 ATTAAT AT^TAAT
AsnI 1011 ATTAAT AT^TAAT
Asp718I 4505 GGTACC G^GTACC
BamHI 5943 GGATCC G^GATCC
BbeI 354 GGCGCC GGCGC^C
BbrPI 4893 CACGTG CAC^GTG
BclI 805 TGATCA T^GATCA
BsaAI 4893 CACGTG [CT]AC^GT[GA]
BseAI 6799 TCCGGA T^CCGGA
BsmBI 5857 GAGACG CGTCTC(1/5)
BsmI 5438 GAATGC GAATGC(1/-1)
BspEI 6799 TCCGGA T^CCGGA
BsrDI 2257 GCAATG GCAATG(2/0)
BssSI 1779 CACGAG CACGAG(-5/-1)
BstEII 5545 GGTGACC G^GT[ACGT]ACC
BstPI 5545 GGTGACC G^GT[ACGT]ACC
EcoO65I 5545 GGTGACC G^GT[ACGT]ACC
EcoRI 1026 GAATTC G^AATTC
EcoRV 4996 GATATC GAT^ATC
FbaI 805 TGATCA T^GATCA
HincII 2830 GTCAAC GT[CT]^[GA]AC
HindII 2830 GTCAAC GT[CT]^[GA]AC
KasI 354 GGCGCC G^GCGCC
KpnI 4505 GGTACC GGTAC^C
MaeII 4894 ACGT A^CGT
MroI 6799 TCCGGA T^CCGGA
NarI 354 GGCGCC GG^CGCC
NheI 4281 GCTAGC G^CTAGC
NspI 2769 GCATGC [GA]CATG^[CT]
PmaCI 4893 CACGTG CAC^GTG
PmlI 4893 CACGTG CAC^GTG
PshAI 2549 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1011 ATTAAT AT^TAAT
SbfI 6024 CCTGCAGG CCTGCA^GG
ScaI 6230 AGTACT AGT^ACT
SexAI 3204 ACCAGGT A^CC[AT]GGT
SfoI 354 GGCGCC GGC^GCC
SgrAI 332 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2769 GCATGC GCATG^C
Sse8387I 6024 CCTGCAGG CCTGCA^GG
SspI 1014 AATATT AAT^ATT
Tsp509I 1027 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccI 6100 GTCTAC GT^[AC][GT]AC
6394 GTCTAC
AvrII 1145 CCTAGG C^CTAGG
4631 CCTAGG
BlnI 1145 CCTAGG C^CTAGG
4631 CCTAGG
BlpI 3179 GCTGAGC GC^T[ACGT]AGC
4453 GCTAAGC
Bpu1102I 3179 GCTGAGC GC^T[ACGT]AGC
4453 GCTAAGC
BsaWI 5434 ACCGGA [AT]^CCGG[AT]
6799 TCCGGA
BstAPI 1994 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4034 GCAGGGGTTGC
CelII 3179 GCTGAGC GC^T[ACGT]AGC
4453 GCTAAGC
DraIII 5029 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5136 CACCCTGTG
SacI 882 GAGCTC GAGCT^C
3065 GAGCTC
SapI 6858 GAAGAGC GCTCTTC(1/4)
4413 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp700I AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI