##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AviII BseAI BsiEI BsiWI
BspDI BspEI BssHII Bst1107I BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1959 GGTACC G^GTACC
AflII 3576 CTTAAG C^TTAAG
Asp718I 1959 GGTACC G^GTACC
AspI 3510 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 5088 GGCGCC GGCGC^C
BbrPI 3069 CACGTG CAC^GTG
BcgI 4404 GCAGCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5775 GGATAC GTATCC(6/5)
BclI 1589 TGATCA T^GATCA
BfrI 3576 CTTAAG C^TTAAG
BsaAI 3069 CACGTG [CT]AC^GT[GA]
BsaWI 5197 TCCGGT [AT]^CCGG[AT]
Bsp1407I 6082 TGTACA T^GTACA
BspHI 1797 TCATGA T^CATGA
BsrFI 4253 GCCGGC [GA]^CCGG[CT]
BsrGI 6082 TGTACA T^GTACA
BssSI 836 CACGAG CACGAG(-5/-1)
BstBI 3679 TTCGAA TT^CGAA
Cfr10I 4253 GCCGGC [GA]^CCGG[CT]
DrdI 5245 GACGGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3811 CCTTGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3861 GAATTC G^AATTC
FbaI 1589 TGATCA T^GATCA
KasI 5088 GGCGCC G^GCGCC
KpnI 1959 GGTACC GGTAC^C
KspI 3024 CCGCGG CCGC^GG
NaeI 4253 GCCGGC GCC^GGC
NarI 5088 GGCGCC GG^CGCC
NgoMIV 4253 GCCGGC G^CCGGC
NheI 2466 GCTAGC G^CTAGC
NspV 3679 TTCGAA TT^CGAA
PflFI 3510 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3069 CACGTG CAC^GTG
PmlI 3069 CACGTG CAC^GTG
RcaI 1797 TCATGA T^CATGA
SacII 3024 CCGCGG CCGC^GG
SapI 201 GCTCTTC GCTCTTC(1/4)
SfoI 5088 GGCGCC GGC^GCC
SfuI 3679 TTCGAA TT^CGAA
SspBI 6082 TGTACA T^GTACA
SspI 6114 AATATT AAT^ATT
Tth111I 3510 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5239 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 5088 GGCGCC G[GA]^CG[CT]C
5269 GACGCC
AflIII 499 ACATGT A^C[GA][CT]GT
2276 ACATGT
AhdI 872 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3594 GACAAGTTGTC
AspEI 872 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3594 GACAAGTTGTC
BamHI 3620 GGATCC G^GATCC
5020 GGATCC
BglII 2141 AGATCT A^GATCT
4809 AGATCT
BsaBI 2142 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4465 GATCACCATC
BsaHI 5088 GGCGCC G[GA]^CG[CT]C
5269 GACGCC
BsmBI 3653 GAGACG CGTCTC(1/5)
5243 GAGACG
BsmI 2945 GAATGC GAATGC(1/-1)
443 GCATTC
BspLU11I 499 ACATGT A^CATGT
2276 ACATGT
BstAPI 370 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4148 GCATCCACTGC
Eam1105I 872 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3594 GACAAGTTGTC
HgaI 5269 GACGC GACGC(5/10)
5640 GACGC
Hin1I 5088 GGCGCC G[GA]^CG[CT]C
5269 GACGCC
MamI 2142 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4465 GATCACCATC
MseI 1440 TTAA T^TAA
3577 TTAA
NcoI 1859 CCATGG C^CATGG
5814 CCATGG
PstI 1737 CTGCAG CTGCA^G
5935 CTGCAG
SmaI 2002 CCCGGG CCC^GGG
2245 CCCGGG
SphI 733 GCATGC GCATG^C
778 GCATGC
Tru9I 1440 TTAA T^TAA
3577 TTAA
XmaCI 2002 CCCGGG C^CCGGG
2245 CCCGGG
XmaI 2002 CCCGGG C^CCGGG
2245 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII