################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII AccI AflII AhdI Aor51HI ApaI AscI AseI AsnI AspEI BfrI BsiEI BsiWI BsmBI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FseI KspI MfeI MluI MunI NheI NotI NruI NspV PacI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 96 GGTACC G^GTACC AccII 704 CGCG CG^CG AccIII 736 TCCGGA T^CCGGA AclI 6751 AACGTT AA^CGTT AcyI 3063 GGCGCC G[GA]^CG[CT]C AgeI 4853 ACCGGT A^CCGGT Asp718I 96 GGTACC G^GTACC AspI 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1036 TGCGCA TGC^GCA BbeI 3063 GGCGCC GGCGC^C BglI 3261 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4152 AGATCT A^GATCT BsaHI 3063 GGCGCC G[GA]^CG[CT]C BseAI 736 TCCGGA T^CCGGA Bsp1407I 6875 TGTACA T^GTACA BspEI 736 TCCGGA T^CCGGA BspLU11I 78 ACATGT A^CATGT BsrDI 1028 CATTGC GCAATG(2/0) BsrGI 6875 TGTACA T^GTACA BstEII 3211 GGTGACC G^GT[ACGT]ACC BstPI 3211 GGTGACC G^GT[ACGT]ACC BstUI 704 CGCG CG^CG DrdI 5541 GACTAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3211 GGTGACC G^GT[ACGT]ACC EcoRV 2623 GATATC GAT^ATC EcoT22I 6510 ATGCAT ATGCA^T FspI 1036 TGCGCA TGC^GCA HgaI 760 GCGTC GACGC(5/10) Hin1I 3063 GGCGCC G[GA]^CG[CT]C KasI 3063 GGCGCC G^GCGCC KpnI 96 GGTACC GGTAC^C MroI 736 TCCGGA T^CCGGA MvnI 704 CGCG CG^CG NarI 3063 GGCGCC GG^CGCC NdeI 1621 CATATG CA^TATG NsiI 6510 ATGCAT ATGCA^T PaeR7I 5556 CTCGAG C^TCGAG PflFI 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4853 ACCGGT A^CCGGT Psp1406I 6751 AACGTT AA^CGTT SacI 6429 GAGCTC GAGCT^C SapI 1451 GCTCTTC GCTCTTC(1/4) SexAI 5917 ACCTGGT A^CC[AT]GGT SfoI 3063 GGCGCC GGC^GCC SmaI 6161 CCCGGG CCC^GGG SpeI 2700 ACTAGT A^CTAGT SspBI 6875 TGTACA T^GTACA SspI 2180 AATATT AAT^ATT Tth111I 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 5556 CTCGAG C^TCGAG XmaCI 6161 CCCGGG C^CCGGG XmaI 6161 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 2085 AAATTC [GA]^AATT[CT] 2708 AAATTC AflIII 78 ACATGT A^C[GA][CT]GT 5155 ACGTGT Alw44I 4200 GTGCAC G^TGCAC 5151 GTGCAC ApaLI 4200 GTGCAC G^TGCAC 5151 GTGCAC ApoI 2085 AAATTC [GA]^AATT[CT] 2708 AAATTC AvaI 5556 CTCGAG C^[CT]CG[GA]G 6161 CCCGGG AvrII 1946 CCTAGG C^CTAGG 2770 CCTAGG BalI 4788 TGGCCA TGG^CCA 6779 TGGCCA BamHI 3450 GGATCC G^GATCC 6068 GGATCC BbrPI 5154 CACGTG CAC^GTG 6345 CACGTG BlnI 1946 CCTAGG C^CTAGG 2770 CCTAGG BlpI 2452 GCTCAGC GC^T[ACGT]AGC 3666 GCTGAGC Bpu1102I 2452 GCTCAGC GC^T[ACGT]AGC 3666 GCTGAGC BsaBI 486 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1148 GATGCCCATC BsmI 4137 GAATGC GAATGC(1/-1) 2511 GCATTC BsoBI 5556 CTCGAG C^[CT]CG[GA]G 6161 CCCGGG BspHI 2174 TCATGA T^CATGA 5096 TCATGA BsrBI 765 CCGCTC CCGCTC(-3/-3) 668 GAGCGG CelII 2452 GCTCAGC GC^T[ACGT]AGC 3666 GCTGAGC HaeII 1804 AGCGCC [GA]GCGC^[CT] 3063 GGCGCC HpaI 2124 GTTAAC GTT^AAC 3151 GTTAAC MamI 486 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1148 GATGCCCATC MluNI 4788 TGGCCA TGG^CCA 6779 TGGCCA MscI 4788 TGGCCA TGG^CCA 6779 TGGCCA NaeI 661 GCCGGC GCC^GGC 890 GCCGGC NcoI 1141 CCATGG C^CATGG 3604 CCATGG NgoMIV 661 GCCGGC G^CCGGC 890 GCCGGC PmaCI 5154 CACGTG CAC^GTG 6345 CACGTG PmlI 5154 CACGTG CAC^GTG 6345 CACGTG RcaI 2174 TCATGA T^CATGA 5096 TCATGA ScaI 946 AGTACT AGT^ACT 4114 AGTACT XbaI 2187 TCTAGA T^CTAGA 3952 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI