##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccI AflII AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
BfrI BsiEI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI KspI MfeI
MluI MunI NheI NotI NruI
NspV PacI PmeI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 96 GGTACC G^GTACC
AccII 704 CGCG CG^CG
AccIII 736 TCCGGA T^CCGGA
AclI 6751 AACGTT AA^CGTT
AcyI 3063 GGCGCC G[GA]^CG[CT]C
AgeI 4853 ACCGGT A^CCGGT
Asp718I 96 GGTACC G^GTACC
AspI 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1036 TGCGCA TGC^GCA
BbeI 3063 GGCGCC GGCGC^C
BglI 3261 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4152 AGATCT A^GATCT
BsaHI 3063 GGCGCC G[GA]^CG[CT]C
BseAI 736 TCCGGA T^CCGGA
Bsp1407I 6875 TGTACA T^GTACA
BspEI 736 TCCGGA T^CCGGA
BspLU11I 78 ACATGT A^CATGT
BsrDI 1028 CATTGC GCAATG(2/0)
BsrGI 6875 TGTACA T^GTACA
BstEII 3211 GGTGACC G^GT[ACGT]ACC
BstPI 3211 GGTGACC G^GT[ACGT]ACC
BstUI 704 CGCG CG^CG
DrdI 5541 GACTAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3211 GGTGACC G^GT[ACGT]ACC
EcoRV 2623 GATATC GAT^ATC
EcoT22I 6510 ATGCAT ATGCA^T
FspI 1036 TGCGCA TGC^GCA
HgaI 760 GCGTC GACGC(5/10)
Hin1I 3063 GGCGCC G[GA]^CG[CT]C
KasI 3063 GGCGCC G^GCGCC
KpnI 96 GGTACC GGTAC^C
MroI 736 TCCGGA T^CCGGA
MvnI 704 CGCG CG^CG
NarI 3063 GGCGCC GG^CGCC
NdeI 1621 CATATG CA^TATG
NsiI 6510 ATGCAT ATGCA^T
PaeR7I 5556 CTCGAG C^TCGAG
PflFI 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4853 ACCGGT A^CCGGT
Psp1406I 6751 AACGTT AA^CGTT
SacI 6429 GAGCTC GAGCT^C
SapI 1451 GCTCTTC GCTCTTC(1/4)
SexAI 5917 ACCTGGT A^CC[AT]GGT
SfoI 3063 GGCGCC GGC^GCC
SmaI 6161 CCCGGG CCC^GGG
SpeI 2700 ACTAGT A^CTAGT
SspBI 6875 TGTACA T^GTACA
SspI 2180 AATATT AAT^ATT
Tth111I 5240 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 5556 CTCGAG C^TCGAG
XmaCI 6161 CCCGGG C^CCGGG
XmaI 6161 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 2085 AAATTC [GA]^AATT[CT]
2708 AAATTC
AflIII 78 ACATGT A^C[GA][CT]GT
5155 ACGTGT
Alw44I 4200 GTGCAC G^TGCAC
5151 GTGCAC
ApaLI 4200 GTGCAC G^TGCAC
5151 GTGCAC
ApoI 2085 AAATTC [GA]^AATT[CT]
2708 AAATTC
AvaI 5556 CTCGAG C^[CT]CG[GA]G
6161 CCCGGG
AvrII 1946 CCTAGG C^CTAGG
2770 CCTAGG
BalI 4788 TGGCCA TGG^CCA
6779 TGGCCA
BamHI 3450 GGATCC G^GATCC
6068 GGATCC
BbrPI 5154 CACGTG CAC^GTG
6345 CACGTG
BlnI 1946 CCTAGG C^CTAGG
2770 CCTAGG
BlpI 2452 GCTCAGC GC^T[ACGT]AGC
3666 GCTGAGC
Bpu1102I 2452 GCTCAGC GC^T[ACGT]AGC
3666 GCTGAGC
BsaBI 486 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1148 GATGCCCATC
BsmI 4137 GAATGC GAATGC(1/-1)
2511 GCATTC
BsoBI 5556 CTCGAG C^[CT]CG[GA]G
6161 CCCGGG
BspHI 2174 TCATGA T^CATGA
5096 TCATGA
BsrBI 765 CCGCTC CCGCTC(-3/-3)
668 GAGCGG
CelII 2452 GCTCAGC GC^T[ACGT]AGC
3666 GCTGAGC
HaeII 1804 AGCGCC [GA]GCGC^[CT]
3063 GGCGCC
HpaI 2124 GTTAAC GTT^AAC
3151 GTTAAC
MamI 486 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1148 GATGCCCATC
MluNI 4788 TGGCCA TGG^CCA
6779 TGGCCA
MscI 4788 TGGCCA TGG^CCA
6779 TGGCCA
NaeI 661 GCCGGC GCC^GGC
890 GCCGGC
NcoI 1141 CCATGG C^CATGG
3604 CCATGG
NgoMIV 661 GCCGGC G^CCGGC
890 GCCGGC
PmaCI 5154 CACGTG CAC^GTG
6345 CACGTG
PmlI 5154 CACGTG CAC^GTG
6345 CACGTG
RcaI 2174 TCATGA T^CATGA
5096 TCATGA
ScaI 946 AGTACT AGT^ACT
4114 AGTACT
XbaI 2187 TCTAGA T^CTAGA
3952 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Asp700I AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI