##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AccIII AflII AgeI Alw44I ApaLI
AscI AvrII BbeI BfrI BglI
BlnI BsaBI BseAI BsiWI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CpoI HpaI KasI MamI MroI
NarI NdeI NheI NotI NspV
PinAI PmeI PshAI PvuI RsrII
SfiI SfoI SfuI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2358 GACGTC GACGT^C
AclI 4333 AACGTT AA^CGTT
AcyI 2358 GACGTC G[GA]^CG[CT]C
Aor51HI 776 AGCGCT AGC^GCT
AviII 1195 TGCGCA TGC^GCA
BamHI 247 GGATCC G^GATCC
BcgI 772 GCAAAGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 2358 GACGTC G[GA]^CG[CT]C
BsiEI 5208 CGGCCG CG[GA][CT]^CG
Bsp1407I 590 TGTACA T^GTACA
BspDI 637 ATCGAT AT^CGAT
BspHI 4948 TCATGA T^CATGA
BsrGI 590 TGTACA T^GTACA
ClaI 637 ATCGAT AT^CGAT
DraIII 5537 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1467 GACGGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5208 CGGCCG C^GGCCG
EclXI 5208 CGGCCG C^GGCCG
Eco47III 776 AGCGCT AGC^GCT
Eco52I 5208 CGGCCG C^GGCCG
EcoRV 2880 GATATC GAT^ATC
EcoT22I 118 ATGCAT ATGCA^T
FseI 1935 GGCCGGCC GGCCGG^CC
FspI 1195 TGCGCA TGC^GCA
Hin1I 2358 GACGTC G[GA]^CG[CT]C
KspI 3610 CCGCGG CCGC^GG
MluI 4505 ACGCGT A^CGCGT
NruI 70 TCGCGA TCG^CGA
NsiI 118 ATGCAT ATGCA^T
PacI 6370 TTAATTAA TTAAT^TAA
PaeR7I 3285 CTCGAG C^TCGAG
Psp1406I 4333 AACGTT AA^CGTT
RcaI 4948 TCATGA T^CATGA
SacII 3610 CCGCGG CCGC^GG
SalI 3153 GTCGAC G^TCGAC
SapI 5505 GAAGAGC GCTCTTC(1/4)
SbfI 4646 CCTGCAGG CCTGCA^GG
ScaI 4279 AGTACT AGT^ACT
SexAI 4659 ACCTGGT A^CC[AT]GGT
SphI 5814 GCATGC GCATG^C
Sse8387I 4646 CCTGCAGG CCTGCA^GG
SspBI 590 TGTACA T^GTACA
XbaI 546 TCTAGA T^CTAGA
XhoI 3285 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 208 GGTACC G^GTACC
1075 GGTACC
AhdI 1203 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4010 GACACCCAGTC
AseI 2637 ATTAAT AT^TAAT
6369 ATTAAT
AsnI 2637 ATTAAT AT^TAAT
6369 ATTAAT
Asp700I 74 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3197 GAATAAGTTC
Asp718I 208 GGTACC G^GTACC
1075 GGTACC
AspEI 1203 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4010 GACACCCAGTC
BglII 229 AGATCT A^GATCT
3571 AGATCT
BlpI 5028 GCTCAGC GC^T[ACGT]AGC
5118 GCTAAGC
Bpu1102I 5028 GCTCAGC GC^T[ACGT]AGC
5118 GCTAAGC
BsaWI 645 ACCGGA [AT]^CCGG[AT]
2926 ACCGGA
BsrBI 1213 CCGCTC CCGCTC(-3/-3)
23 GAGCGG
BssSI 1985 CACGAG CACGAG(-5/-1)
5903 CACGAG
BstAPI 2585 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4994 GCAGACCCTGC
CelII 5028 GCTCAGC GC^T[ACGT]AGC
5118 GCTAAGC
DraI 4148 TTTAAA TTT^AAA
6455 TTTAAA
Eam1105I 1203 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4010 GACACCCAGTC
EcoNI 1514 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2327 CCTCTGCAAGG
HindIII 302 AAGCTT A^AGCTT
4061 AAGCTT
KpnI 208 GGTACC GGTAC^C
1075 GGTACC
MfeI 2162 CAATTG C^AATTG
2242 CAATTG
MunI 2162 CAATTG C^AATTG
2242 CAATTG
NaeI 1936 GCCGGC GCC^GGC
3243 GCCGGC
NgoMIV 1936 GCCGGC G^CCGGC
3243 GCCGGC
PshBI 2637 ATTAAT AT^TAAT
6369 ATTAAT
SmaI 243 CCCGGG CCC^GGG
6019 CCCGGG
SspI 1974 AATATT AAT^ATT
6153 AATATT
StuI 3229 AGGCCT AGG^CCT
3748 AGGCCT
XmaCI 243 CCCGGG C^CCGGG
6019 CCCGGG
XmaI 243 CCCGGG C^CCGGG
6019 CCCGGG
XmnI 74 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3197 GAATAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII