##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
AatII AclI AgeI AscI AviII
AvrII BbrPI BlnI BsiWI BssHII
Bst1107I BstZ17I CpoI DraIII FseI
FspI HpaI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SexAI SgrAI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 6183 GGTACC G^GTACC
AccI 3233 GTCTAC GT^[AC][GT]AC
AccIII 5391 TCCGGA T^CCGGA
Alw44I 5854 GTGCAC G^TGCAC
Aor51HI 356 AGCGCT AGC^GCT
ApaLI 5854 GTGCAC G^TGCAC
Asp718I 6183 GGTACC G^GTACC
AspI 4691 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 524 GGATCC G^GATCC
BclI 3506 TGATCA T^GATCA
BsaBI 4193 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5391 TCCGGA [AT]^CCGG[AT]
BseAI 5391 TCCGGA T^CCGGA
BsgI 4573 CTGCAC GTGCAG(16/14)
BsiEI 324 CGGCCG CG[GA][CT]^CG
Bsp1407I 3722 TGTACA T^GTACA
BspDI 1111 ATCGAT AT^CGAT
BspEI 5391 TCCGGA T^CCGGA
BsrDI 1526 CATTGC GCAATG(2/0)
BsrGI 3722 TGTACA T^GTACA
BstBI 3110 TTCGAA TT^CGAA
BstEII 468 GGTAACC G^GT[ACGT]ACC
BstPI 468 GGTAACC G^GT[ACGT]ACC
ClaI 1111 ATCGAT AT^CGAT
EagI 324 CGGCCG C^GGCCG
EclXI 324 CGGCCG C^GGCCG
Eco47III 356 AGCGCT AGC^GCT
Eco52I 324 CGGCCG C^GGCCG
EcoO65I 468 GGTAACC G^GT[ACGT]ACC
EcoT22I 3069 ATGCAT ATGCA^T
FbaI 3506 TGATCA T^GATCA
KpnI 6183 GGTACC GGTAC^C
KspI 463 CCGCGG CCGC^GG
MamI 4193 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 375 ACGCGT A^CGCGT
MroI 5391 TCCGGA T^CCGGA
NdeI 4965 CATATG CA^TATG
NsiI 3069 ATGCAT ATGCA^T
NspV 3110 TTCGAA TT^CGAA
PaeR7I 857 CTCGAG C^TCGAG
PflFI 4691 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 463 CCGCGG CCGC^GG
ScaI 3126 AGTACT AGT^ACT
SfiI 1080 GGCCTCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3110 TTCGAA TT^CGAA
SspBI 3722 TGTACA T^GTACA
Tth111I 4691 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 857 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 3256 CTTAAG C^TTAAG
4481 CTTAAG
AhdI 5676 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6490 GACAGCTTGTC
ApaI 4429 GGGCCC GGGCC^C
5881 GGGCCC
AseI 2798 ATTAAT AT^TAAT
3906 ATTAAT
AsnI 2798 ATTAAT AT^TAAT
3906 ATTAAT
AspEI 5676 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6490 GACAGCTTGTC
BcgI 3112 CGAAGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5863 CGAGGAGAATGC
BciVI 644 GGATAC GTATCC(6/5)
1611 GTATCC
BfrI 3256 CTTAAG C^TTAAG
4481 CTTAAG
BglI 1072 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1081 GCCTCCACGGC
BsaAI 580 TACGTA [CT]AC^GT[GA]
3888 TACGTA
BsmBI 21 CGTCTC CGTCTC(1/5)
6148 CGTCTC
BspLU11I 3215 ACATGT A^CATGT
3413 ACATGT
BsrFI 121 GCCGGC [GA]^CCGG[CT]
234 GCCGGC
Cfr10I 121 GCCGGC [GA]^CCGG[CT]
234 GCCGGC
DrdI 1311 GACACCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3594 GACGTTGGGGTC
Eam1105I 5676 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6490 GACAGCTTGTC
EarI 4543 CTCTTC CTCTTC(1/4)
909 GAAGAG
HincII 2365 GTTGAC GT[CT]^[GA]AC
2915 GTTGAC
HindII 2365 GTTGAC GT[CT]^[GA]AC
2915 GTTGAC
Ksp632I 4543 CTCTTC CTCTTC(1/4)
909 GAAGAG
MfeI 3029 CAATTG C^AATTG
3283 CAATTG
MunI 3029 CAATTG C^AATTG
3283 CAATTG
NaeI 121 GCCGGC GCC^GGC
234 GCCGGC
NgoMIV 121 GCCGGC G^CCGGC
234 GCCGGC
PflMI 4677 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5070 CCAGTGTGTGG
PshBI 2798 ATTAAT AT^TAAT
3906 ATTAAT
PvuII 3731 CAGCTG CAG^CTG
6626 CAGCTG
SacI 2162 GAGCTC GAGCT^C
5604 GAGCTC
SapI 909 GAAGAGC GCTCTTC(1/4)
4542 GCTCTTC
SnaBI 580 TACGTA TAC^GTA
3888 TACGTA
SpeI 3857 ACTAGT A^CTAGT
4303 ACTAGT
SphI 5004 GCATGC GCATG^C
5032 GCATGC
Van91I 4677 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5070 CCAGTGTGTGG
XbaI 1176 TCTAGA T^CTAGA
2389 TCTAGA
XcmI 608 CCAACAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2140 CCACATCTTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI