##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII AcsI AgeI Aor51HI
ApoI AscI Asp718I AviII BbrPI
BclI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI KpnI
MamI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SalI ScaI SfuI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1971 GACGTC GACGT^C
AccI 3356 GTCTAC GT^[AC][GT]AC
AclI 282 AACGTT AA^CGTT
AflII 4880 CTTAAG C^TTAAG
AhdI 2396 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 951 ATTAAT AT^TAAT
AsnI 951 ATTAAT AT^TAAT
AspEI 2396 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2714 GCTGAGG CCTCAGC(-5/-2)
BfrI 4880 CTTAAG C^TTAAG
BsaI 129 GGTCTC GGTCTC(1/5)
BsmI 795 GAATGC GAATGC(1/-1)
BssHII 5502 GCGCGC G^CGCGC
BstEII 4603 GGTGACC G^GT[ACGT]ACC
BstPI 4603 GGTGACC G^GT[ACGT]ACC
DrdI 3347 GACGACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2396 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4603 GGTGACC G^GT[ACGT]ACC
HpaI 1147 GTTAAC GTT^AAC
KspI 2527 CCGCGG CCGC^GG
NdeI 5649 CATATG CA^TATG
PshBI 951 ATTAAT AT^TAAT
Psp1406I 282 AACGTT AA^CGTT
SacII 2527 CCGCGG CCGC^GG
SapI 4727 GAAGAGC GCTCTTC(1/4)
SfiI 3149 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 4712 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 618 TACGTA TAC^GTA
StuI 1610 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 3061 GTGCAC G^TGCAC
5490 GTGCAC
AocI 1965 CCTCAGG CC^T[ACGT]AGG
5225 CCTCAGG
ApaLI 3061 GTGCAC G^TGCAC
5490 GTGCAC
Asp700I 1462 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5736 GAATGTTTTC
BamHI 1407 GGATCC G^GATCC
4846 GGATCC
BbeI 2855 GGCGCC GGCGC^C
3780 GGCGCC
BciVI 2502 GGATAC GTATCC(6/5)
2949 GTATCC
BlpI 1494 GCTCAGC GC^T[ACGT]AGC
5305 GCTCAGC
Bpu1102I 1494 GCTCAGC GC^T[ACGT]AGC
5305 GCTCAGC
Bsp1407I 5645 TGTACA T^GTACA
5853 TGTACA
BspLU11I 3621 ACATGT A^CATGT
5460 ACATGT
BsrBI 4117 CCGCTC CCGCTC(-3/-3)
3374 GAGCGG
BsrDI 4707 CATTGC GCAATG(2/0)
557 GCAATG
BsrGI 5645 TGTACA T^GTACA
5853 TGTACA
Bsu36I 1965 CCTCAGG CC^T[ACGT]AGG
5225 CCTCAGG
CelII 1494 GCTCAGC GC^T[ACGT]AGC
5305 GCTCAGC
DraIII 3917 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5323 CACTTGGTG
Eco81I 1965 CCTCAGG CC^T[ACGT]AGG
5225 CCTCAGG
HindIII 40 AAGCTT A^AGCTT
1415 AAGCTT
KasI 2855 GGCGCC G^GCGCC
3780 GGCGCC
NarI 2855 GGCGCC GG^CGCC
3780 GGCGCC
NcoI 515 CCATGG C^CATGG
2652 CCATGG
SacI 1378 GAGCTC GAGCT^C
5075 GAGCTC
SbfI 1310 CCTGCAGG CCTGCA^GG
3559 CCTGCAGG
SexAI 3918 ACCTGGT A^CC[AT]GGT
4368 ACCTGGT
SfoI 2855 GGCGCC GGC^GCC
3780 GGCGCC
Sse8387I 1310 CCTGCAGG CCTGCA^GG
3559 CCTGCAGG
SspBI 5645 TGTACA T^GTACA
5853 TGTACA
SspI 376 AATATT AAT^ATT
5996 AATATT
TaqI 387 TCGA T^CGA
2513 TCGA
TthHB8I 387 TCGA T^CGA
2513 TCGA
XmnI 1462 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5736 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AspI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BglII BlnI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI XhoII XmaCI XmaI