##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BcgI BlnI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
HpaI KpnI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PmeI PshAI PshBI PvuI
RsrII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3339 GACGTC GACGT^C
AccI 1315 GTCTAC GT^[AC][GT]AC
AclI 3616 AACGTT AA^CGTT
AcsI 5374 AAATTC [GA]^AATT[CT]
AflII 2884 CTTAAG C^TTAAG
AgeI 1732 ACCGGT A^CCGGT
ApoI 5374 AAATTC [GA]^AATT[CT]
Asp700I 679 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 6126 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1835 GGATCC G^GATCC
BclI 4760 TGATCA T^GATCA
BfrI 2884 CTTAAG C^TTAAG
BglII 1279 AGATCT A^GATCT
BsaBI 424 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1732 ACCGGT [AT]^CCGG[AT]
BsiEI 4092 CGACCG CG[GA][CT]^CG
BsmBI 1844 GAGACG CGTCTC(1/5)
BsrDI 1485 GCAATG GCAATG(2/0)
BssSI 635 CACGAG CACGAG(-5/-1)
DrdI 1214 GACCTGCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 4760 TGATCA T^GATCA
HindIII 4737 AAGCTT A^AGCTT
KspI 935 CCGCGG CCGC^GG
MamI 424 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 5961 CCATGG C^CATGG
PaeR7I 6135 CTCGAG C^TCGAG
PflFI 6126 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1732 ACCGGT A^CCGGT
Psp1406I 3616 AACGTT AA^CGTT
SacII 935 CCGCGG CCGC^GG
SbfI 4831 CCTGCAGG CCTGCA^GG
SexAI 1626 ACCTGGT A^CC[AT]GGT
SphI 4914 GCATGC GCATG^C
Sse8387I 4831 CCTGCAGG CCTGCA^GG
Tth111I 6126 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 6135 CTCGAG C^TCGAG
XmnI 679 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 4804 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACTTAATGTC
AspEI 4804 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACTTAATGTC
BciVI 287 GTATCC GTATCC(6/5)
5266 GTATCC
Bsp1407I 2610 TGTACA T^GTACA
3736 TGTACA
BspHI 328 TCATGA T^CATGA
4210 TCATGA
BsrGI 2610 TGTACA T^GTACA
3736 TGTACA
BstEII 898 GGTCACC G^GT[ACGT]ACC
1031 GGTGACC
BstPI 898 GGTCACC G^GT[ACGT]ACC
1031 GGTGACC
DsaI 935 CCGCGG C^C[GA][CT]GG
5961 CCATGG
Eam1105I 4804 GACAGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACTTAATGTC
EcoO65I 898 GGTCACC G^GT[ACGT]ACC
1031 GGTGACC
EcoT22I 1253 ATGCAT ATGCA^T
4912 ATGCAT
NaeI 149 GCCGGC GCC^GGC
2066 GCCGGC
NdeI 2616 CATATG CA^TATG
4631 CATATG
NgoMIV 149 GCCGGC G^CCGGC
2066 GCCGGC
NsiI 1253 ATGCAT ATGCA^T
4912 ATGCAT
NspI 2682 GCATGT [GA]CATG^[CT]
4914 GCATGC
PflMI 2247 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4936 CCAGTGCCTGG
RcaI 328 TCATGA T^CATGA
4210 TCATGA
SapI 787 GCTCTTC GCTCTTC(1/4)
6010 GCTCTTC
SmaI 1657 CCCGGG CCC^GGG
2382 CCCGGG
SspBI 2610 TGTACA T^GTACA
3736 TGTACA
SspI 5129 AATATT AAT^ATT
6068 AATATT
Van91I 2247 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4936 CCAGTGCCTGG
XmaCI 1657 CCCGGG C^CCGGG
2382 CCCGGG
XmaI 1657 CCCGGG C^CCGGG
2382 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII