##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AflII AocI Aor51HI AscI
Asp700I Asp718I AspI BbrPI BbvCI
BfrI BlpI Bpu1102I BsaI BsiWI
BsmBI BspDI BssHII Bsu36I CelII
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI KpnI KspI MfeI
MluI MunI NaeI NgoMIV NotI
NruI NsiI PflFI PmaCI PmeI
PmlI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfiI
SmaI Sse8387I SwaI Tth111I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 122 GACGTC GACGT^C
AccII 1676 CGCG CG^CG
AhdI 756 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2720 GTGCAC G^TGCAC
ApaI 1259 GGGCCC GGGCC^C
ApaLI 2720 GTGCAC G^TGCAC
AspEI 756 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3530 TGCGCA TGC^GCA
AvrII 6053 CCTAGG C^CTAGG
BamHI 1475 GGATCC G^GATCC
BanI 153 GGCGCC G^G[CT][GA]CC
BbeI 153 GGCGCC GGCGC^C
BciVI 4219 GTATCC GTATCC(6/5)
BglI 145 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6053 CCTAGG C^CTAGG
BsaAI 4420 TACGTA [CT]AC^GT[GA]
BsiEI 159 CGACCG CG[GA][CT]^CG
Bsp1407I 3282 TGTACA T^GTACA
BsrBI 107 GAGCGG CCGCTC(-3/-3)
BsrGI 3282 TGTACA T^GTACA
Bst1107I 1788 GTATAC GTA^TAC
BstAPI 3574 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1758 TTCGAA TT^CGAA
BstEII 1637 GGTTACC G^GT[ACGT]ACC
BstPI 1637 GGTTACC G^GT[ACGT]ACC
BstUI 1676 CGCG CG^CG
BstZ17I 1788 GTATAC GTA^TAC
DrdI 288 GACACCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 756 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1637 GGTTACC G^GT[ACGT]ACC
FspI 3530 TGCGCA TGC^GCA
HgaI 877 GACGC GACGC(5/10)
KasI 153 GGCGCC G^GCGCC
MspA1I 178 CCGCTG C[AC]G^C[GT]G
MvnI 1676 CGCG CG^CG
NarI 153 GGCGCC GG^CGCC
NdeI 5585 CATATG CA^TATG
NspV 1758 TTCGAA TT^CGAA
PacI 2120 TTAATTAA TTAAT^TAA
PaeR7I 5161 CTCGAG C^TCGAG
PpuMI 229 AGGACCC [GA]G^G[AT]CC[CT]
SapI 1251 GAAGAGC GCTCTTC(1/4)
SexAI 3856 ACCAGGT A^CC[AT]GGT
SfoI 153 GGCGCC GGC^GCC
SfuI 1758 TTCGAA TT^CGAA
SgrAI 1538 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 4420 TACGTA TAC^GTA
SpeI 3951 ACTAGT A^CTAGT
SphI 3067 GCATGC GCATG^C
SspBI 3282 TGTACA T^GTACA
XhoI 5161 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 119 GTAGAC GT^[AC][GT]AC
1788 GTATAC
AccIII 1940 TCCGGA T^CCGGA
2024 TCCGGA
AclI 3009 AACGTT AA^CGTT
5295 AACGTT
AcyI 122 GACGTC G[GA]^CG[CT]C
153 GGCGCC
AgeI 486 ACCGGT A^CCGGT
1539 ACCGGT
AlwNI 1798 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2417 CAGTCTCTG
AseI 2119 ATTAAT AT^TAAT
4837 ATTAAT
AsnI 2119 ATTAAT AT^TAAT
4837 ATTAAT
BalI 394 TGGCCA TGG^CCA
1847 TGGCCA
BanII 1259 GGGCCC G[GA]GC[CT]^C
5101 GAGCCC
BcgI 128 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1200 CGACCTGACTGC
BclI 806 TGATCA T^GATCA
1805 TGATCA
BsaHI 122 GACGTC G[GA]^CG[CT]C
153 GGCGCC
BseAI 1940 TCCGGA T^CCGGA
2024 TCCGGA
BsmI 2300 GAATGC GAATGC(1/-1)
5798 GAATGC
BspEI 1940 TCCGGA T^CCGGA
2024 TCCGGA
BspHI 551 TCATGA T^CATGA
4280 TCATGA
BspMI 2014 ACCTGC ACCTGC(4/8)
817 GCAGGT
BsrDI 4701 CATTGC GCAATG(2/0)
3612 GCAATG
BsrFI 486 ACCGGT [GA]^CCGG[CT]
1539 ACCGGT
BssSI 72 CTCGTG CACGAG(-5/-1)
97 CTCGTG
BstXI 1158 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2307 CCACTTATCTGG
Cfr10I 486 ACCGGT [GA]^CCGG[CT]
1539 ACCGGT
EaeI 394 TGGCCA [CT]^GGCC[GA]
1847 TGGCCA
EarI 4868 CTCTTC CTCTTC(1/4)
1251 GAAGAG
EcoRI 1272 GAATTC G^AATTC
5417 GAATTC
FbaI 806 TGATCA T^GATCA
1805 TGATCA
HaeII 20 GGCGCT [GA]GCGC^[CT]
153 GGCGCC
Hin1I 122 GACGTC G[GA]^CG[CT]C
153 GGCGCC
HpaI 1128 GTTAAC GTT^AAC
1488 GTTAAC
Ksp632I 4868 CTCTTC CTCTTC(1/4)
1251 GAAGAG
MluNI 394 TGGCCA TGG^CCA
1847 TGGCCA
MroI 1940 TCCGGA T^CCGGA
2024 TCCGGA
MscI 394 TGGCCA TGG^CCA
1847 TGGCCA
NcoI 1850 CCATGG C^CATGG
4006 CCATGG
NheI 2203 GCTAGC G^CTAGC
2263 GCTAGC
PflMI 1642 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6144 CCAAGCCGTGG
PinAI 486 ACCGGT A^CCGGT
1539 ACCGGT
PshAI 204 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4284 GACCCTAGTC
PshBI 2119 ATTAAT AT^TAAT
4837 ATTAAT
Psp1406I 3009 AACGTT AA^CGTT
5295 AACGTT
RcaI 551 TCATGA T^CATGA
4280 TCATGA
StuI 750 AGGCCT AGG^CCT
1892 AGGCCT
Van91I 1642 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6144 CCAAGCCGTGG
XcmI 170 CCATCGGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1158 CCAAGAGTCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII