##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AgeI Alw44I Aor51HI ApaLI
BbeI BcgI BlpI Bpu1102I BsiWI
BsmBI BspDI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraIII DrdI
Eco47III EcoNI EcoRV FseI HpaI
KasI MluI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SalI SfiI SfoI SfuI SnaBI
StuI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 6407 GGTACC G^GTACC
AclI 5946 AACGTT AA^CGTT
AcyI 142 GGCGTC G[GA]^CG[CT]C
AhdI 5448 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4353 GGGCCC GGGCC^C
AscI 47 GGCGCGCC GG^CGCGCC
AseI 309 ATTAAT AT^TAAT
AsnI 309 ATTAAT AT^TAAT
Asp718I 6407 GGTACC G^GTACC
AspEI 5448 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5819 TGCGCA TGC^GCA
AvrII 5708 CCTAGG C^CTAGG
BalI 5024 TGGCCA TGG^CCA
BbsI 4420 GAAGAC GAAGAC(2/6)
BciVI 666 GTATCC GTATCC(6/5)
BclI 6237 TGATCA T^GATCA
BglI 1908 GCCAGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5708 CCTAGG C^CTAGG
BpuAI 4420 GAAGAC GAAGAC(2/6)
BsaBI 2793 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 142 GGCGTC G[GA]^CG[CT]C
BsiEI 224 CGGCCG CG[GA][CT]^CG
Bsp1407I 2040 TGTACA T^GTACA
BsrBI 1306 CCGCTC CCGCTC(-3/-3)
BsrGI 2040 TGTACA T^GTACA
BstEII 1885 GGTTACC G^GT[ACGT]ACC
BstPI 1885 GGTTACC G^GT[ACGT]ACC
EagI 224 CGGCCG C^GGCCG
Eam1105I 5448 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 224 CGGCCG C^GGCCG
Eco52I 224 CGGCCG C^GGCCG
EcoO65I 1885 GGTTACC G^GT[ACGT]ACC
FbaI 6237 TGATCA T^GATCA
FspI 5819 TGCGCA TGC^GCA
HaeII 937 AGCGCC [GA]GCGC^[CT]
Hin1I 142 GGCGTC G[GA]^CG[CT]C
HindIII 7011 AAGCTT A^AGCTT
KpnI 6407 GGTACC GGTAC^C
MamI 2793 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 5024 TGGCCA TGG^CCA
MscI 5024 TGGCCA TGG^CCA
NdeI 6344 CATATG CA^TATG
PshAI 6142 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 309 ATTAAT AT^TAAT
Psp1406I 5946 AACGTT AA^CGTT
SbfI 6411 CCTGCAGG CCTGCA^GG
SexAI 4690 ACCAGGT A^CC[AT]GGT
SgrAI 1373 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2175 CCCGGG CCC^GGG
SphI 249 GCATGC GCATG^C
Sse8387I 6411 CCTGCAGG CCTGCA^GG
SspBI 2040 TGTACA T^GTACA
SwaI 2535 ATTTAAAT ATTT^AAAT
XmaCI 2175 CCCGGG C^CCGGG
XmaI 2175 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3039 GTAGAC GT^[AC][GT]AC
6149 GTCTAC
AccIII 5232 TCCGGA T^CCGGA
5430 TCCGGA
AflII 3133 CTTAAG C^TTAAG
5536 CTTAAG
AocI 925 CCTCAGG CC^T[ACGT]AGG
4522 CCTTAGG
AspI 4423 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4463 GACTGGGTC
AvaI 74 CTCGGG C^[CT]CG[GA]G
2175 CCCGGG
BamHI 481 GGATCC G^GATCC
6108 GGATCC
BbrPI 4708 CACGTG CAC^GTG
5831 CACGTG
BbvCI 5224 CCTCAGC CCTCAGC(-5/-2)
5358 CCTCAGC
BfrI 3133 CTTAAG C^TTAAG
5536 CTTAAG
BsaWI 5232 TCCGGA [AT]^CCGG[AT]
5430 TCCGGA
BseAI 5232 TCCGGA T^CCGGA
5430 TCCGGA
BsoBI 74 CTCGGG C^[CT]CG[GA]G
2175 CCCGGG
BspEI 5232 TCCGGA T^CCGGA
5430 TCCGGA
BsrFI 1374 GCCGGT [GA]^CCGG[CT]
6717 ACCGGC
BssHII 48 GCGCGC G^CGCGC
132 GCGCGC
Bsu36I 925 CCTCAGG CC^T[ACGT]AGG
4522 CCTTAGG
Cfr10I 1374 GCCGGT [GA]^CCGG[CT]
6717 ACCGGC
Eco81I 925 CCTCAGG CC^T[ACGT]AGG
4522 CCTTAGG
EcoRI 2286 GAATTC G^AATTC
3071 GAATTC
EcoT22I 3720 ATGCAT ATGCA^T
6525 ATGCAT
HgaI 143 GCGTC GACGC(5/10)
6518 GCGTC
KspI 56 CCGCGG CCGC^GG
137 CCGCGG
MfeI 614 CAATTG C^AATTG
7163 CAATTG
MroI 5232 TCCGGA T^CCGGA
5430 TCCGGA
MunI 614 CAATTG C^AATTG
7163 CAATTG
NcoI 193 CCATGG C^CATGG
1853 CCATGG
NsiI 3720 ATGCAT ATGCA^T
6525 ATGCAT
PflFI 4423 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4463 GACTGGGTC
PmaCI 4708 CACGTG CAC^GTG
5831 CACGTG
PmlI 4708 CACGTG CAC^GTG
5831 CACGTG
SacII 56 CCGCGG CCGC^GG
137 CCGCGG
SpeI 1597 ACTAGT A^CTAGT
2939 ACTAGT
TaqI 1145 TCGA T^CGA
5736 TCGA
Tth111I 4423 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4463 GACTGGGTC
TthHB8I 1145 TCGA T^CGA
5736 TCGA
XbaI 2955 TCTAGA T^CTAGA
6564 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BanII BbvI
BcnI BfaI BglII BmyI BpmI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspLU11I BspMI
BsrDI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII XmnI