##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AscI Asp718I AspEI AspI
AviII BbeI BciVI BclI BsaBI
BsaHI BseAI BsiWI BspDI BspEI
BssHII BssSI BstBI BstEII BstPI
ClaI CpoI Eam1105I EcoO65I EcoRI
EcoRV FbaI FspI Hin1I HpaI
KasI KpnI MamI MluI MroI
NarI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PvuI RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Aor51HI 28 AGCGCT AGC^GCT
AseI 2789 ATTAAT AT^TAAT
AsnI 2789 ATTAAT AT^TAAT
Asp700I 4004 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2050 TGGCCA TGG^CCA
BamHI 2846 GGATCC G^GATCC
BcgI 3132 CGATTAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2676 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 301 AGATCT A^GATCT
BsiEI 860 CGGCCG CG[GA][CT]^CG
Bsp1407I 5289 TGTACA T^GTACA
BsrGI 5289 TGTACA T^GTACA
BstAPI 3846 GCAACACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 860 CGGCCG C^GGCCG
EclXI 860 CGGCCG C^GGCCG
Eco47III 28 AGCGCT AGC^GCT
Eco52I 860 CGGCCG C^GGCCG
EcoT22I 1305 ATGCAT ATGCA^T
FseI 4571 GGCCGGCC GGCCGG^CC
HgaI 1699 GCGTC GACGC(5/10)
HindIII 4970 AAGCTT A^AGCTT
KspI 2905 CCGCGG CCGC^GG
MfeI 635 CAATTG C^AATTG
MluNI 2050 TGGCCA TGG^CCA
MscI 2050 TGGCCA TGG^CCA
MunI 635 CAATTG C^AATTG
NsiI 1305 ATGCAT ATGCA^T
PshBI 2789 ATTAAT AT^TAAT
SacI 2876 GAGCTC GAGCT^C
SacII 2905 CCGCGG CCGC^GG
SapI 2827 GCTCTTC GCTCTTC(1/4)
SnaBI 2087 TACGTA TAC^GTA
SspBI 5289 TGTACA T^GTACA
XmnI 4004 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 4270 GTATAC GT^[AC][GT]AC
5297 GTATAC
AclI 612 AACGTT AA^CGTT
3329 AACGTT
AflII 5171 CTTAAG C^TTAAG
5505 CTTAAG
Alw44I 247 GTGCAC G^TGCAC
3043 GTGCAC
ApaLI 247 GTGCAC G^TGCAC
3043 GTGCAC
BanI 924 GGCACC G^G[CT][GA]CC
4869 GGTGCC
BbsI 5266 GAAGAC GAAGAC(2/6)
3288 GTCTTC
BbvCI 2727 CCTCAGC CCTCAGC(-5/-2)
2144 GCTGAGG
BfrI 5171 CTTAAG C^TTAAG
5505 CTTAAG
BpuAI 5266 GAAGAC GAAGAC(2/6)
3288 GTCTTC
BsaWI 545 ACCGGA [AT]^CCGG[AT]
4882 TCCGGT
BsmBI 1700 CGTCTC CGTCTC(1/5)
2070 CGTCTC
BsmI 1750 GAATGC GAATGC(1/-1)
4247 GAATGC
BspHI 2038 TCATGA T^CATGA
3477 TCATGA
BsrBI 863 CCGCTC CCGCTC(-3/-3)
3481 GAGCGG
Bst1107I 4270 GTATAC GTA^TAC
5297 GTATAC
BstZ17I 4270 GTATAC GTA^TAC
5297 GTATAC
DraI 2388 TTTAAA TTT^AAA
5050 TTTAAA
DrdI 2235 GACTCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2429 GACACAGCAGTC
NaeI 53 GCCGGC GCC^GGC
4572 GCCGGC
NgoMIV 53 GCCGGC G^CCGGC
4572 GCCGGC
PflMI 725 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCATTCCGTGG
Psp1406I 612 AACGTT AA^CGTT
3329 AACGTT
RcaI 2038 TCATGA T^CATGA
3477 TCATGA
SexAI 625 ACCTGGT A^CC[AT]GGT
3679 ACCAGGT
SfaNI 3744 GATGC GCATC(5/9)
1436 GCATC
Van91I 725 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2404 CCATTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BanII BbrPI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI