##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
BbrPI BclI BfrI BsaWI BseAI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI EcoRI FbaI FseI
HpaI KpnI KspI MluI MroI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SacII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccII 277 CGCG CG^CG
AhdI 3645 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2339 GTGCAC G^TGCAC
Aor51HI 5625 AGCGCT AGC^GCT
ApaLI 2339 GTGCAC G^TGCAC
AspEI 3645 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 692 GGCGCC GGCGC^C
BsaAI 3594 CACGTA [CT]AC^GT[GA]
BsiWI 3596 CGTACG C^GTACG
Bsp1407I 4442 TGTACA T^GTACA
BsrGI 4442 TGTACA T^GTACA
BstAPI 6405 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 277 CGCG CG^CG
CpoI 256 CGGACCG CG^G[AT]CCG
DraIII 4929 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1621 GACAGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3645 GACCACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5625 AGCGCT AGC^GCT
EcoT22I 4603 ATGCAT ATGCA^T
KasI 692 GGCGCC G^GCGCC
MfeI 622 CAATTG C^AATTG
MunI 622 CAATTG C^AATTG
MvnI 277 CGCG CG^CG
NarI 692 GGCGCC GG^CGCC
NcoI 2010 CCATGG C^CATGG
NheI 102 GCTAGC G^CTAGC
NsiI 4603 ATGCAT ATGCA^T
RsrII 256 CGGACCG CG^G[AT]CCG
SfiI 3033 GGCCAAACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 692 GGCGCC GGC^GCC
SspBI 4442 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 226 GTAGAC GT^[AC][GT]AC
1234 GTAGAC
AcyI 692 GGCGCC G[GA]^CG[CT]C
930 GACGCC
AflIII 3656 ACATGT A^C[GA][CT]GT
5640 ACATGT
AviII 1433 TGCGCA TGC^GCA
3368 TGCGCA
AvrII 5435 CCTAGG C^CTAGG
5945 CCTAGG
BbvCI 2004 CCTCAGC CCTCAGC(-5/-2)
6441 CCTCAGC
BcgI 2560 CGATATTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3318 GCAGATGATTCG
BciVI 531 GGATAC GTATCC(6/5)
3951 GGATAC
BlnI 5435 CCTAGG C^CTAGG
5945 CCTAGG
BsaBI 3324 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6710 GATATGAATC
BsaHI 692 GGCGCC G[GA]^CG[CT]C
930 GACGCC
BsiEI 3399 CGGCCG CG[GA][CT]^CG
4335 CGGCCG
BsmBI 6347 GAGACG CGTCTC(1/5)
6512 GAGACG
BspHI 1362 TCATGA T^CATGA
3332 TCATGA
BspLU11I 3656 ACATGT A^CATGT
5640 ACATGT
BsrBI 4338 CCGCTC CCGCTC(-3/-3)
4363 GAGCGG
BsrDI 50 GCAATG GCAATG(2/0)
5214 GCAATG
BstEII 3471 GGTGACC G^GT[ACGT]ACC
4774 GGTGACC
BstPI 3471 GGTGACC G^GT[ACGT]ACC
4774 GGTGACC
EagI 3399 CGGCCG C^GGCCG
4335 CGGCCG
EclXI 3399 CGGCCG C^GGCCG
4335 CGGCCG
Eco52I 3399 CGGCCG C^GGCCG
4335 CGGCCG
EcoO65I 3471 GGTGACC G^GT[ACGT]ACC
4774 GGTGACC
EcoRV 1063 GATATC GAT^ATC
5281 GATATC
FspI 1433 TGCGCA TGC^GCA
3368 TGCGCA
HgaI 930 GACGC GACGC(5/10)
2730 GCGTC
Hin1I 692 GGCGCC G[GA]^CG[CT]C
930 GACGCC
HincII 1159 GTCAAC GT[CT]^[GA]AC
3805 GTTGAC
HindII 1159 GTCAAC GT[CT]^[GA]AC
3805 GTTGAC
HindIII 2287 AAGCTT A^AGCTT
2485 AAGCTT
MamI 3324 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6710 GATATGAATC
RcaI 1362 TCATGA T^CATGA
3332 TCATGA
SapI 2048 GAAGAGC GCTCTTC(1/4)
4287 GCTCTTC
SexAI 1817 ACCTGGT A^CC[AT]GGT
1889 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI